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The utility of yeast as a tool for cell-based, target-directed high-throughput screening

  • J. L. NORCLIFFE (a1), E. ALVAREZ-RUIZ (a2), J. J. MARTIN-PLAZA (a2), P. G. STEEL (a1) and P. W. DENNY (a1) (a3)...

Summary

Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.

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Corresponding author

*Corresponding authors: Department of Chemistry, Biophysical Sciences Institute, Durham, DH1 3LE, UK. Tel: +44 (0)191 334 3983. Fax: +44 (0)191 334 2051. E-mail: p.w.denny@durham.ac.uk, E-mail: p.g.steel@durham.ac.uk. Tel: +44 (0)191 324 2131.

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