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Toxocara canis: anthelmintic activity of quinone derivatives in murine toxocarosis

Published online by Cambridge University Press:  18 February 2016

T. MATA-SANTOS ,
H. A. MATA-SANTOS ,
P. F. CARNEIRO ,
K. C. G. DE MOURA ,
J. M. FENALTI ,
G. B. KLAFKE ,
L. A. X. CRUZ ,
L. H. R. MARTINS ,
N. F. PINTO  and
M. C. F. R. PINTO
...Show all authors
T. MATA-SANTOS*
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
H. A. MATA-SANTOS
Affiliation:
Universidade Federal do Rio de Janeiro – Faculdade de Farmácia – Departamento de Análises Clínicas e Toxicológicas, Rio de Janeiro, Brazil
P. F. CARNEIRO
Affiliation:
Universidade Federal do Rio de Janeiro – Faculdade de Farmácia – Instituto de Pesquisa de Produtos Naturais, Rio de Janeiro, Brazil
K. C. G. DE MOURA
Affiliation:
Universidade Federal do Rio de Janeiro – Faculdade de Farmácia – Instituto de Pesquisa de Produtos Naturais, Rio de Janeiro, Brazil
J. M. FENALTI
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
G. B. KLAFKE
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
L. A. X. CRUZ
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
L. H. R. MARTINS
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
N. F. PINTO
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
M. C. F. R. PINTO
Affiliation:
Universidade Federal do Rio de Janeiro – Faculdade de Farmácia – Instituto de Pesquisa de Produtos Naturais, Rio de Janeiro, Brazil
M. E. A. BERNE
Affiliation:
Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas – Instituto de Biologia, Pelotas, Brazil
P. E. A. DA SILVA
Affiliation:
Universidade Federal do Rio Grande –Núcleo de Pesquisa e Microbiologia Médica – Faculdade de Medicina, Rio Grande, Brazil
C. J. SCAINI
Affiliation:
Universidade Federal do Rio Grande – Faculdade de Medicina – Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rio Grande, Brazil
*
*Corresponding author: Universidade Federal do Rio Grande, Faculdade de Medicina, Área Interdisciplinar em Ciências Biomédicas – Laboratório de Parasitologia, Rua General Osório s/n, Área Acadêmica do Hospital Universitário, Rio Grande, RS 96200-190, Brazil. E-mail: farmatais@hotmail.com
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Summary

Human toxocarosis is a chronic tissue parasitosis most often caused by Toxocara canis. The seroprevalence can reach up to 50%, especially among children and adolescents. The anthelmintics used in the treatment have moderate efficacy. The aim of this study was to evaluate the in vitro and in vivo anthelmintic activity of quinones and their derivatives against T. canis larvae and the cytotoxicity of the larvicidal compounds. The compounds were evaluated at 1 mg mL−1 concentration in microculture plates containing third stage larvae in an Roswell Park Memorial Institute (RPMI) 1640 environment, incubated at 37 °C in 5% CO2 tension for 48 h. Five naphthoxiranes were selected for the cytotoxicity analysis. The cell viability evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays using murine peritoneal macrophages isolated from C57BL/6 mice revealed that the naphthoxiranes (1 and 3) were less cytotoxic at a concentration of 0·05 mg mL−1. The efficacy of naphthoxiranes (1 and 3) was examined in murine toxocarosis also. The anthelmintic activity was examined by evaluating the number of larvae in the brain, carcass, liver, lungs, heart, kidneys and eyes. Compound (3) demonstrated anthelmintic activity similar to that of albendazole by decreasing the number of larvae in the organs of mice and thus could form the basis of the development of a new anthelmintic drug.

Keywords

Toxocariasisnaphthoxiraneslarvicidalchemotherapy
Type
Research Article
Information
Parasitology , Volume 143 , Issue 4 , April 2016 , pp. 507 - 517
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Copyright
Copyright © Cambridge University Press 2016 

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