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Tissue eosinophilia correlates with mice susceptibility, granuloma formation and damage during Toxocara canis infection

Published online by Cambridge University Press:  10 February 2022

Thaís Leal-Silva ,
Camila de Almeida Lopes ,
Flaviane Vieira-Santos ,
Fabrício Marcus Silva Oliveira ,
Lucas Kraemer ,
Luiza de Lima Silva Padrão ,
Chiara Cássia Oliveira Amorim ,
Jorge Lucas Nascimento Souza ,
Fernando Sérgio Barbosa  and
Milene Alvarenga Rachid
...Show all authors
Thaís Leal-Silva
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil Post-graduation Program in Health Sciences: Infectious Diseases and Tropical Medicine, Faculdade de Medicina, Federal University of Minas Gerais, Belo Horizonte, Brazil
Camila de Almeida Lopes
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Flaviane Vieira-Santos
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Fabrício Marcus Silva Oliveira
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Lucas Kraemer
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Luiza de Lima Silva Padrão
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil Post-graduation Program in Health Sciences: Infectious Diseases and Tropical Medicine, Faculdade de Medicina, Federal University of Minas Gerais, Belo Horizonte, Brazil
Chiara Cássia Oliveira Amorim
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Jorge Lucas Nascimento Souza
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Fernando Sérgio Barbosa
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Milene Alvarenga Rachid
Affiliation:
Laboratory of Protozooses, Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Remo Castro Russo
Affiliation:
Laboratory of Pulmonary Immunology and Mechanics, Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Ricardo Toshio Fujiwara
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil Post-graduation Program in Health Sciences: Infectious Diseases and Tropical Medicine, Faculdade de Medicina, Federal University of Minas Gerais, Belo Horizonte, Brazil
Lilian Lacerda Bueno*
Affiliation:
Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil Post-graduation Program in Health Sciences: Infectious Diseases and Tropical Medicine, Faculdade de Medicina, Federal University of Minas Gerais, Belo Horizonte, Brazil
*
Author for correspondence: Lilian Lacerda Bueno, E-mail: llbueno@icb.ufmg.br
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Abstract

An increase in peripheral blood eosinophils in helminth infections is expected, and these cells are known to promote immunity against these parasites. However, studies have suggested that in some specific helminths, eosinophils may promote the needs and longevity of these parasites, and their role in these infections remains undefined, including in Toxocara canis infection. Thus, this study aimed to investigate the role of eosinophils in the context of larval migration of T. canis and the immunopathological aspects of infection. For this, we used wild-type mice and mice genetically deficient for the transcription factor GATA-binding factor 1 (GATA1−/−), infected with 1000 eggs of T. canis. At 0, 3, 14 and 63 days post-infection, parasite load, tissue cytokine production, leucocyte profile, bronchoalveolar lavage cells and histopathological analyses were carried out. Collectively, our results demonstrate that the presence of eosinophils mediates susceptibility to T. canis, inducing leucocytosis and the formation of granulomas, increasing the pulmonary and cerebral parasite load, and reducing the number of neutrophils, which may be necessary to control the infection.

Keywords

EosinophilGATA-1granulomaimmunologytissue inflammationtoxocariasis
Type
Research Article
Information
Parasitology , Volume 149 , Issue 7 , June 2022 , pp. 893 - 904
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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