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Studies on immunity to Schistosoma mansoni in vivo: whole-body irradiation has no effect on vaccine-induced resistance in mice

Published online by Cambridge University Press:  06 April 2009

D. A. A. Vignali ,
Q. D. Bickle  and
M. G. Taylor
D. A. A. Vignali
Affiliation:
Department of Medical Helminthology, Winches Farm Laboratories, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts., AL4 0XQ
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, Winches Farm Laboratories, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts., AL4 0XQ
M. G. Taylor
Affiliation:
Department of Medical Helminthology, Winches Farm Laboratories, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts., AL4 0XQ
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Summary

Actively immunized mice, whole-body irradiated with 650 or 525 rad., manifested comparable levels of resistance to Schistosoma mansoni compared with unirradiated, immunized mice in spite of a marked reduction in circulating leucocytes (>90%) and platelets (>85%), and despite an abrogation of delayed-type hypersensitivity (DTH) (Type IV) response to schistosomular antigens (as determined by footpad swelling, 24 h after injection of antigen). However, limited histopathological comparison of lung sections from irradiated and unirradiated mice 7 days post-challenge showed that cellular reactions (‘foci’) around parasites were essentially similar in size and cellular composition except that in irradiated mice, eosinophils were poorly represented both in the foci and in lung tissue in general. Neither presumed immune complex-mediated (Type III, Arthus reaction) hypersensitivity (as determined by footpad swelling, 5 h after injection of antigen) nor serum anti-schistosomulum extract antibody levels (as determined by ELISA) were affected. In addition, the pattern of 125I-labelled schistosomular surface antigens immunoprecipitated with serum from irradiated and unirradiated mice was essentially similar. These results are consistent with antibody playing an important role in vaccine-induced immunity in mice but suggest that radiosensitive T cell function and radiosensitive cells, such as platelets and polymorphonuclear cells, including eosinophils, may not be essential.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 49 - 61
Copyright
Copyright © Cambridge University Press 1988

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