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Schistosoma mansoni: in vivo and in vitro studies of immunity using the guinea-pig model

Published online by Cambridge University Press:  06 April 2009

E. J. Pearce  and
Diane J. McLaren
E. J. Pearce
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Diane J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

In vivo and in vitro parameters of immunity have been assessed in guinea-pigs sensitized with 500 normal or 500 radiation-attenuated cercariae of Schistosoma mansoni. High levels of resistance to a challenge infection developed in both the chronic and irradiated vaccine model, but immunity was expressed earlier (week 4) and reached higher levels (90%) in the latter case. Vaccinated guinea-pigs have thus been shown to achieve greater resistance than the more commonly used rodent hosts. In vitro cytotoxicity assays have demonstrated that antibodies capable of participating in complement-dependent (lethal antibody) or eosinophil-mediated schistosomular killing, develop in the serum of guinea-pigs immunized with either normal or irradiated cercariae. The time course of development of the eosinophil adherence promoting antibody approximated in both models, the development of immunity in vivo, but the lethal antibody response paralleled the immune status of the animal only in the irradiated vaccine model

Type
Research Article
Information
Parasitology , Volume 87 , Issue 3 , December 1983 , pp. 465 - 479
Copyright
Copyright © Cambridge University Press 1983

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References

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