Hostname: page-component-84b7d79bbc-g5fl4 Total loading time: 0 Render date: 2024-07-26T18:25:39.344Z Has data issue: false hasContentIssue false
  • English
  • Français

Radio isotope studies on the blood loss associated with Nippostrongylus brasiliensis infection in rats

Published online by Cambridge University Press:  06 April 2009

J. T. McL. Neilson
J. T. McL. Neilson
Affiliation:
University of Glasgow Veterinary School
Get access Rights & Permissions [Opens in a new window]

Extract

Red blood cells from rats were labelled with 51Cr by in vitro incubation with labelled sodium chromate. After re-injection of autologous labelled red cells, rats, each infected with 5000 N. brasiliensis larvae, and non-infected controls were placed in individual metabolism cages. Twenty-four hour samples of faeces and urine from both groups were collected throughout the course of the infection. From a knowledge of the specific radioactivity of host blood, it was possible to express the radioactivity found in the faeces and urine in terms of volume of whole blood. A burden of approximately 1000 adult worms caused a blood loss of about 100 μl every 24 h during the period day 4 to day 14 after infection. This gastro-intestinal haemorrhage could provide a vehicle whereby antibody gains access to the worm. No radioactivity was found in adult worms isolated from infected rats killed at 2-day intervals throughout the course of the infection. Adult N. brasiliensis did not ingest an appreciable quantity of host blood.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 1 , February 1969 , pp. 123 - 127
Copyright
Copyright © Cambridge University Press 1969

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barth, E. E. E., Jarrett, W. F. H. & Urquhart, G. M. (1966). Studies on the mechanism of the self-cure reactions in rats infected with Nippostrongylus brasiliensis. Immunology 10, 459–64.Google Scholar
Chandler, A. C. (1937). Studies on the nature of immunity to intestinal helminths. VI. General résumé and discussion. Am. J. Hyg. 26, 309–21.Google Scholar
Haley, A. J. (1962). Biology of the rat nematode, Nippostrongylus brasiliensis (Travassos, 1914). II. Preparasitie stages and development in the laboratory rat. J. Parasit. 48, 13–23.CrossRefGoogle ScholarPubMed
Hughes-Jones, N. C. & Cheney, B. (1961). The use of 51Cr and 59Fe as red cell labels to determine the fate of normal erythrocytes in the rat. Clin. Sci. 20, 323–32.Google Scholar
Jennings, F. W. (1962). Biochemical and immunological studies on some helminth diseases of domestic animals Ph.D. Thesis, University of Glasgow.Google Scholar
Jennings, F. W., Mulligan, W. & Urquhart, G. M. (1956). Radioisotope studies on the anemia produced by infection with Fasciola hepatica. Expl Parasit. 5, 458–68.Google Scholar
Miller, T. A. (1966). Blood loss during hookworm infection, determined by erythrocyte labelling with radioactive 51chromium. I. Infection of dogs with normal and with X-irradiated Ancylostoma caninum. J. Parasit. 52, 844–55.CrossRefGoogle ScholarPubMed
Mollison, P. L. & Veall, N. (1955). The use of the isotope 51Cr as a label for red cells. Br. J. Haemat. 1, 62–74.Google Scholar
Mulligan, W., Urquhart, G. M., Jennings, F. W. & Neilson, J. T. M. (1965). Immunological studies on Nippostrongylus brasiliensis infection in the rat: the ‘self-cure’ phenomenon. Expl Parasit. 16, 341–7.CrossRefGoogle ScholarPubMed
Owen, C. A., Bollman, J. & Grindlay, J. H. (1954). Radiochromium-labelled erythrocytes for the detection of gastrointestinal hemorrhage. J. Lab. clin. Med. 44, 238–45.Google Scholar
Pearson, I. G. (1963). Use of the chromium radioisotope 51Cr to estimate blood loss through ingestion by Fasciola heptica. Expl Parasit. 13, 186–93.Google Scholar
Rogers, W. P. & Lazarus, M. (1949). The uptake of radioactive phosphorus from host tissues and fluids by nematode parasites. Parasitology 39, 245–50.Google Scholar
Sarles, M. P. & Taliaferro, W. H. (1936). The local points of defense and the passive transfer of acquired immunity to Nippostrongylus muris in rats. J. infect. Dis. 59, 207–20.CrossRefGoogle Scholar
Taliaferro, W. H. (1940). The mechanism of acquired immunity in infections with parasitic worms. Physiol. Rev. 20, 469–92.CrossRefGoogle Scholar
Taliaferro, W. H., & Sarles, M. P. (1939). The cellular reactions in the skin, lungs and intestine of normal and immune rats after infection with Nippostrongylus muris. J. infect. Dis. 64, 157–92.CrossRefGoogle Scholar
Weinstein, P. P. & Jones, M. F. (1956). The in vitro cultivation of Nippostrongylus muris to the adult stage. J. Parasit. 42, 215–36.CrossRefGoogle Scholar