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Presence of Trypanosoma cruzi antigen on the surface of both infected and uninfected cells in tissue culture

Published online by Cambridge University Press:  06 April 2009

I. A. Abrahamsohn  and
J. K. Kloetzel
I. A. Abrahamsohn
Affiliation:
Instituto de Ciências Biomédicas da Universidade de São Paulo, Departamento de Medicina Preventiva, Faculdad de Medicina da Universidade de São Paulo and Instituto de Medicina Tropical de São Paulo, Av. Dr Enéas de Carvalho Aguiar 470, Caixa Postal 2921, São Paulo, Brasil
J. K. Kloetzel
Affiliation:
Instituto de Ciências Biomédicas da Universidade de São Paulo, Departamento de Medicina Preventiva, Faculdad de Medicina da Universidade de São Paulo and Instituto de Medicina Tropical de São Paulo, Av. Dr Enéas de Carvalho Aguiar 470, Caixa Postal 2921, São Paulo, Brasil
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Summary

LLC-MK2 cell monolayers infected with Trypanosoma cruzi were shown by immunofluorescence to present parasite antigens on the surface of both parasitized and non-parasitized cells after completion of the first intracellular cycle and rupture of infected cells. The cell-culture supernatant fluid at this stage, as well as the supernatant fluid of parasites left overnight in culture medium were concentrated and contained antigen capable of binding to uninfected cell monolayers. The origin of this antigen, as well as its eventual role in the pathogenesis of Chagas' disease, are discussed.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 147 - 152
Copyright
Copyright © Cambridge University Press 1980

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