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Population dynamics of Theileria sergenti in persistently infected cattle and vector ticks analysed by a polymerase chain reaction

Published online by Cambridge University Press:  06 April 2009

S. Kubota ,
C. Sugimoto  and
M. Onuma
S. Kubota
Affiliation:
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
C. Sugimoto
Affiliation:
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
M. Onuma*
Affiliation:
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
*
* Corresponding author.
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Summary

Theileria sergenti Shintoku stock consists of 2 parasite populations bearing 2 atlelic forms of p33/32, an immunodominant piroplasm surface protein. Parasite population changes during parasite passages among cattle and tick vectors, and during persistent infection in individual calves were analysed by using allele-specific polymerase chain reaction (PCR). The parasite DNAs were prepared from piroplasms from calves which had been infected with Shintoku stock by inoculation of sporozoite stabilates or parasitized erythrocytes, and from sporozoite stabilates which had been prepared from Shintoku stock-infected ticks. Changes in a dominant parasite population were demonstrated during transmission from calves to vector ticks and from infected ticks to calves. Parasite population changes were also apparent during persistent infection in cattle over several months, and this change is thought to occur under host immune pressure. The results of this study indicate that expression of diverse forms of p33/32 may play a role in parasite persistence within mammalian hosts and its transmission from tick vector.

Keywords

antigenic diversityApicomplexapiroplasmaPCRTheileria sergentitick.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 5 , May 1996 , pp. 437 - 442
Copyright
Copyright © Cambridge University Press 1996

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