Skip to main content Accessibility help
×
Home

Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China

  • Nan Liang (a1) (a2) (a3), Yayun Wu (a1) (a2), Mingfei Sun (a4), Yankai Chang (a1) (a2), Xuhui Lin (a4), Linzeng Yu (a4), Suhui Hu (a1) (a2), Xiangqian Zhang (a1) (a2), Shuangjian Zheng (a1) (a2), Zhaohui Cui (a1) (a2) and Longxian Zhang (a1) (a2)...

Abstract

To determine the prevalence of Cryptosporidium in dairy cattle in Guangdong Province, South China, 1440 fecal samples were collected from 10 farms and screened for Cryptosporidium with PCR. The overall prevalence of Cryptosporidium was 4.38% (63/1440), and the infection rates in preweaned calves, postweaned calves, heifers and adults were 6.4% (19/297), 6.19% (33/533), 1.48% (4/271) and 2.06% (7/339), respectively. Three Cryptosporidium species, Cryptosporidium andersoni (n = 33), Cryptosporidium bovis (n = 22) and Cryptosporidium ryanae (n = 8) were detected by DNA sequence analysis of the 63 positive samples, and C. andersoni was identified as the most common species on the dairy cattle farms. In preweaned calves, C. bovis was the most prevalent species (9/19, 47.4%). In contrast, C. andersoni was the predominant species (19/33, 57.6%) in postweaned calves and the only species found in heifers and adults. The zoonotic species Cryptosporidium parvum was not detected in this study. Twenty-four C. andersoni isolates were successfully classified into three multilocus sequence typing (MLST) subtypes. MLST subtype A4,A4,A4,A1 was the predominant subtype, and MLST subtype A2,A5,A2,A1, previously found in sheep, was detected in cattle for the first time. A linkage disequilibrium analysis showed that the C. andersoni isolates had a clonal genetic population structure. However, further molecular studies are required to better understand the epidemiology of Cryptosporidium in Guangdong.

Copyright

Corresponding author

Author for correspondence: Longxian Zhang, E-mail: zhanglx8999@henau.edu.cn and zhanglx8999@gmail.com

References

Hide All
Abeywardena, H, Jex, AR, Koehler, AV, Rajapakse, RP, Udayawarna, K, Haydon, SR, Stevens, MA and Gasser, RB (2014) First molecular characterization of Cryptosporidium and Giardia from bovines (Bos taurus and Bubalus bubalis) in Sri Lanka: unexpected absence of C. parvum from pre-weaned calves. Parasites & Vectors 7, 75.
Alves, M, Xiao, L, Sulaiman, I, Lal, AA, Matos, O and Antunes, F (2003) Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. Journal of Clinical Microbiology 41, 27442747.
Amer, S, Zidan, S, Adamu, H, Ye, J, Roellig, D, Xiao, L and Feng, Y (2013) Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta Provinces, Egypt. Experimental Parasitology 135, 518523.
Baldursson, S and Karanis, P (2011) Waterborne transmission of protozoan parasites: review of worldwide outbreaks – an update 2004–2010. Water Research 45, 66036614.
Blackburn, BG, Mazurek, JM, Hlavsa, M, Park, J and Tillapaw, M (2006) Cryptosporidiosis associated with ozonated apple cider. Emerging Infectious Diseases 12, 684686.
Burenbaatar, B, Bakheit, M, Plutzer, J, Suzuki, N, Igarashi, I, Ongerth, J and Karanis, P (2008) Prevalence and genotyping of Cryptosporidium species from animals in Mongolia. Parasitology Research 102, 901905.
Chen, F and Huang, KH (2012) Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle from farms in China. Journal of Veterinary Science 13, 1522.
Čondlová, Š, Horčičková, M, Sak, B, Květoňová, D, Hlásková, L, Konečný, R, Stanko, M, McEvoy, J and Kváč, M (2018) Cryptosporidium apodemi sp. n. and Cryptosporidium ditrichi sp. n. (Apicomplexa: Cryptosporidiidae) in Apodemus spp. European Journal of Protistology 63, 112.
Cui, Z, Wang, R, Huang, J, Wang, H, Zhao, J and Luo, N (2014) Cryptosporidiosis caused by Cryptosporidium parvum subtype IIdA15G1 at a dairy farm in northwestern China. Parasites & Vectors 7, 14.
Enemark, HL, Ahrens, P, Lowery, CJ, Thamsborg, SM and Enemark, JM (2002) Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation. Veterinary Parasitology 107, 3749.
Esteban, E and Anderson, BC (1995) Cryptosporidium muris: prevalence, persistency, and detrimental effect on milk production in a drylot dairy. Journal of Dairy Science 78, 10681072.
Fan, Y, Wang, T, Koehler, AV, Hu, M and Gasser, RB (2017) Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China. Parasites & Vectors 10, 519.
Fayer, R (2010) Taxonomy and species delimitation in Cryptosporidium. Experimental Parasitology 124, 9097.
Fayer, R, Santín, M, Trout, JM and Greiner, E (2006) Prevalence of species and genotypes of Cryptosporidium found in 1–2-year-old dairy cattle in the eastern United States. Veterinary Parasitology 135, 105112.
Feng, Y, Ortega, Y, He, G, Das, P, Xu, M, Zhang, X, Fayer, R, Gatei, W, Cama, V and Xiao, L (2007) Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Veterinary Parasitology 144, 19.
Feng, Y, Yang, W, Ryan, U, Zhang, L, Kvác, M, Koudela, B, Modrý, D, Li, N, Fayer, R and Xiao, L (2011) Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni. Journal of Clinical Microbiology 49, 3441.
Feng, Y, Karna, SR, Dearen, TK, Singh, DK, Adhikari, LN, Shrestha, A and Xiao, L (2012) Common occurrence of a unique Cryptosporidium ryanae variant in zebu cattle and water buffaloes in the buffer zone of the Chitwan National Park, Nepal. Veterinary Parasitology 185, 309314.
Haubold, B and Hudson, RR (2000) LIAN 3.0: detecting linkage disequilibrium in multilocus data. Bioinformatics (Oxford, England) 16, 847848.
Huang, J, Yue, D, Qi, M, Wang, R, Zhao, J, Li, J, Shi, K, Wang, M and Zhang, L (2014) Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China. BMC Veterinary Research 10, 292.
Imre, K, Lobo, LM, Matos, O, Popescu, C, Genchi, C and Darabus, G (2011) Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania: is there an actual risk of zoonotic infections? Veterinary Parasitology 181, 321324.
Jiang, Y, Ren, J, Yuan, Z, Liu, A, Zhao, H, Liu, H, Chu, L, Pan, W, Cao, J, Lin, Y and Shen, Y (2014) Cryptosporidium andersoni as a novel predominant Cryptosporidium species in outpatients with diarrhea in JiangSu Province, China. BMC Infectious Diseases 14, 555.
Kváč, M, Havrdová, N, Hlásková, L, Daňová, T, Kanděra, J, Ježková, J, Vítovec, J, Sak, B, Ortega, Y, Xiao, L, Modrý, D, Chelladurai, JR, Prantlová, V and McEvoy, J (2016) Cryptosporidium proliferans n. sp. (Apicomplexa: cryptosporidiidae): molecular and biological evidence of cryptic species within gastric Cryptosporidium of mammals. PLoS ONE 11, e0147090.
Kváč, M, Vlnatá, G, Ježková, J, Horčičková, M, Konečný, R, Hlásková, L, McEvoy, J and Sak, B (2018) Cryptosporidium occultus sp. n. (Apicomplexa: Cryptosporidiidae) in rats. European Journal of Protistology 63, 96104.
Leoni, F, Amar, C, Nichols, G, Pedraza-Diaz, S and McLauchlin, J (2006) Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000. Journal of Medical Microbiology 55, 703707.
Liu, A, Wang, R, Li, Y, Zhang, L, Shu, J, Zhang, W, Feng, Y, Xiao, L and Ling, H (2009) Prevalence and distribution of Cryptosporidium spp. In dairy cattle in Heilongjiang Province, China. Parasitology Research 105, 797802.
Ma, J, Li, P, Zhao, X, Xu, H, Wu, W, Wang, Y, Guo, Y, Wang, L, Feng, Y and Xiao, L (2015) Occurrence and molecular characterization of Cryptosporidium spp. and Enterocytozoon bieneusi in dairy cattle, beef cattle and water buffaloes in China. Veterinary Parasitology 209, 220–207.
Maikai, BV, Umoh, JU, Kwaga, JK, Lawal, IA, Maikai, VA, Cama, V and Xiao, L (2011) Molecular characterization of Cryptosporidium spp. in native breeds of cattle in Kaduna State, Nigeria. Veterinary Parasitology 178, 241245.
Murakoshi, F, Xiao, L, Matsubara, R, Sato, R, Kato, Y, Sasaki, T, Fukuda, Y, Tada, C and Nakai, Y (2012) Molecular characterization of Cryptosporidium spp. in grazing beef cattle in Japan. Veterinary Parasitology 187, 123128.
Nguyen, ST, Fukuda, Y, Tada, C, Sato, R, Duong, B, Nguyen, DT and Nakai, Y (2012) Molecular characterization of Cryptosporidium in native beef calves in central Vietnam. Parasitology Research 111, 18171820.
Ondráčková, Z, Kváč, M, Sak, B, Květoňová, D and Rost, M (2009) Prevalence and molecular characterization of Cryptosporidium spp. In dairy cattle in south Bohemia, the Czech Republic. Veterinary Parasitology 165, 141144.
Qi, M, Wang, H, Jing, B, Wang, D, Wang, R and Zhang, L (2015 a) Occurrence and molecular identification of Cryptosporidium spp. in dairy calves in Xinjiang, northwestern China. Veterinary Parasitology 212, 404.
Qi, M, Wang, R, Jing, B, Jian, F, Ning, C and Zhang, L (2016) Prevalence and multilocus genotyping of Cryptosporidium andersoni in dairy cattle and He cattle in Xinjiang, China. Infection Genetics and Evolution 44, 313317.
Qi, MZ, Fang, YQ, Wang, XT, Zhang, LX, Wang, RJ, Du, SZ, Guo, YZ, Jia, YQ, Yao, L, Liu, QD and Zhao, GH (2015 b) Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China. Journal of Medical Microbiology 64, 111116.
Rieux, A, Chartier, C, Pors, I and Paraud, C (2013) Dynamics of excretion and molecular characterization of Cryptosporidium isolates in pre-weaned French beef calves. Veterinary Parasitology 195, 169172.
Ryan, U, Fayer, R and Xiao, L (2014) Cryptosporidium species in humans and animals: current understanding and research needs. Parasitology 141, 16671685.
Santín, M, Trout, JM, Xiao, L, Zhou, L, Greiner, E and Fayer, R (2004) Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Veterinary Parasitology 122, 103117.
Santín, M, Trout, JM and Fayer, R (2008) A longitudinal study of cryptosporidiosis in dairy cattle from birth to 2 years of age. Veterinary Parasitology 155, 1523.
Silverlas, C, Naslund, K, Bjorkman, C and Mattsson, JG (2010) Molecular characterization of Cryptosporidium isolates from Swedish dairy cattle in relation to age, diarrhoea and region. Veterinary Parasitology 169, 289295.
Trout, JM and Santín, M (2008) Livestock. In Fayer, R and Xiao, L (eds), Cryptosporidium and Cryptosporidiosis. 2nd Edn. Boca Raton: CRC Press and IWA Publishing, pp. 451483.
Wang, R, Ma, G, Zhao, J, Lu, Q, Wang, H, Zhang, L, Jian, F, Ning, C and Xiao, L (2011 a) Cryptosporidium andersoni is the predominant species in post-weaned and adult dairy cattle in China. Parasitology International 60, 14.
Wang, R, Wang, H, Sun, Y, Zhang, L, Jian, F, Qi, M, Ning, C and Xiao, L (2011 b) Characteristics of Cryptosporidium transmission in preweaned dairy cattle in Henan, China. Journal of Clinical Microbiology 49, 10771082.
Wang, R, Jian, F, Zhang, L, Ning, C, Liu, A, Zhao, J, Feng, Y, Qi, M, Wang, H, Lv, C, Zhao, G and Xiao, L (2012) Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni. PLoS ONE 7, e43782.
Wegayehu, T, Karim, MR, Anberber, M, Adamu, H, Erko, B, Zhang, L and Tilahun, G (2016) Prevalence and genetic characterization of Cryptosporidium species in dairy calves in Central Ethiopia. PLoS ONE 11, e0154647.
Xiao, L (2010) Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology 124, 8089.
Xiao, L and Feng, Y (2008) Zoonotic cryptosporidiosis. FEMS Immunology and Medical Microbiology 52, 309323.
Zahedi, A, Durmic, Z, Gofton, AW, Kueh, S, Austen, J, Lawson, M, Callahan, L, Jardine, J and Ryan, U (2017) Cryptosporidium homai n.sp. (Apicomplexa: Cryptosporidiiae) from the guinea pig (Cavia porcellus). Veterinary Parasitology 245, 92101.
Zhang, W, Wang, R, Yang, F, Zhang, L, Cao, J, Zhang, X, Hong, L, Liu, A and Shen, Y (2013) Distribution and genetic characterizations of Cryptosporidium spp. in pre-weaned dairy calves in northeastern China's Heilongjiang Province. PLoS ONE 8, e54857.
Zhang, XX, Tan, QD, Zhou, DH, Ni, XT, Liu, GX, Yang, YC and Zhu, XQ (2015) Prevalence and molecular characterization of Cryptosporidium spp. In dairy cattle, northwest China. Parasitology Research 114, 27812787.
Zhao, GH, Ren, WX, Gao, M, Bian, QQ, Hu, B, Cong, MM, Lin, Q, Wang, RJ, Qi, M, Qi, MZ, Zhu, XQ and Zhang, LX (2013) Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China. PLoS ONE 8, e60112.
Zhao, W, Wang, R, Zhang, W, Liu, A, Cao, J, Shen, Y, Yang, F and Zhang, L (2014) MLST subtypes and population genetic structure of Cryptosporidium andersoni from dairy cattle and beef cattle in northeastern China's Heilongjiang Province. PLoS ONE 9, e102006.

Keywords

Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China

  • Nan Liang (a1) (a2) (a3), Yayun Wu (a1) (a2), Mingfei Sun (a4), Yankai Chang (a1) (a2), Xuhui Lin (a4), Linzeng Yu (a4), Suhui Hu (a1) (a2), Xiangqian Zhang (a1) (a2), Shuangjian Zheng (a1) (a2), Zhaohui Cui (a1) (a2) and Longxian Zhang (a1) (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed