Skip to main content Accessibility help
×
Home

A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

  • J. P. DALTON (a1) (a2), S. R. DAY (a1), A. C. DREW (a1) and P. J. BRINDLEY (a1)

Abstract

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

Copyright

Corresponding author

School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland. Tel: +353 1 704 5407. Fax: +353 1 704 5412. E-mail: daltonj@ccmail.dcu.ie.

Keywords

A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

  • J. P. DALTON (a1) (a2), S. R. DAY (a1), A. C. DREW (a1) and P. J. BRINDLEY (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed