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Mesocestoides corti: Morphological features and glycogen mobilization during in vitro differentiation from larva to adult worm

Published online by Cambridge University Press:  09 October 2009

G. CABRERA ,
I. ESPINOZA ,
U. KEMMERLING  and
N. GALANTI
G. CABRERA
Affiliation:
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile
I. ESPINOZA
Affiliation:
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile
U. KEMMERLING
Affiliation:
Programa de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile Facultad de Ciencias de la Salud, Universidad de Talca, Chile
N. GALANTI*
Affiliation:
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile
*
*Corresponding author: Norbel Galanti, Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Casilla 70061, Santiago, Chile. Fax: 56-2-7355580, Tel.: 56-2-6786475, E-mail: ngalanti@med.uchile.cl
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Summary

Mesocestodes corti has the capacity to develop from the tetrathyridium (larva) stage to adult worm in vitro by trypsin and serum stimulation. Consequently, it has been used as an experimental model system for studying cestode development, host-parasite relationships and anthelmintic drugs. We describe morphological features in 5 different developmental stages of M. corti obtained in vitro, including larvae from the peritoneal cavity of infected mice, trypsin- and serum-stimulated larvae, elongated parasites as well as segmented and mature worms. It is unambiguously confirmed that sexually mature worms are obtained as a result of this in vitro process of differentiation. Defined cellular regions are present in all stages of development studied, some of them surrounded by a basal lamina. Glycogen is present in the larvae obtained from the mouse peritoneal cavity and in parasites encapsulated in the mouse host liver. Glycogen distribution in the parasite changes on trypsin and serum stimulation to differentiate. We propose that changes in the distribution of neutral polysaccharides in the parenchyma of the parasite at different stages of development and degradation of polysaccharides in the transition from segmented to adult worm are related to energy needs necessary for the cellular processes leading to the mature specimen.

Keywords

M. cortidevelopmentcell territoriesglycogen
Type
Research Article
Information
Parasitology , Volume 137 , Special Issue 3: Development and applications of cestode and trematode laboratory models , March 2010 , pp. 373 - 384
Copyright
Copyright © Cambridge University Press 2009

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