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Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

  • ADRIANA SEIXAS (a1), ANDRÉIA B. ESTRELA (a1), JULIANA C. CEOLATO (a1), EMERSON G. PONTES (a2), FLÁVIO LARA (a3) (a4), KATIA C. GONDIM (a2) and CARLOS TERMIGNONI (a1) (a3)...

Summary

The tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite's physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis in R. microplus eggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. In R. microplus female gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.

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Corresponding author

*Corresponding author: Avenida Bento Gonçalves 9500, Prédio 43431, Sala 217, Campus do Vale, Caixa Postal 15005, CEP 91501-970, Porto Alegre, RS, Brazil. Tel: +55 51 33086082. Fax: +55 51 33087309. E-mail: adriana@cbiot.ufrgs.br

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Keywords

Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

  • ADRIANA SEIXAS (a1), ANDRÉIA B. ESTRELA (a1), JULIANA C. CEOLATO (a1), EMERSON G. PONTES (a2), FLÁVIO LARA (a3) (a4), KATIA C. GONDIM (a2) and CARLOS TERMIGNONI (a1) (a3)...

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