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The latest FAD – Faecal antibody detection in cattle. Protocol and results from three UK beef farms naturally infected with gastrointestinal nematodes

  • A. S. Cooke (a1) (a2), K. A. Watt (a3), E. R. Morgan (a2) (a4) and J. A. J. Dungait (a1)

Abstract

Antibodies at gastrointestinal mucosal membranes play a vital role in immunological protection against a range of pathogens, including helminths. Gastrointestinal health is central to efficient livestock production, and such infections cause significant losses. Fecal samples were taken from 114 cattle, across three beef farms, with matched blood samples taken from 22 of those animals. To achieve fecal antibody detection, a novel fecal supernatant was extracted. Fecal supernatant and serum samples were then analysed, using adapted enzyme-linked immunosorbent assay protocols, for levels of total immunoglobulin (Ig)A, IgG, IgM, and Teladorsagia circumcincta-specific IgA, IgG, IgM and IgE (in the absence of reagents for cattle-specific nematode species). Fecal nematode egg counts were conducted on all fecal samples. Assays performed successfully and showed that IgA was the predominant antibody in fecal samples, whereas IgG was predominant in serum. Total IgA in feces and serum correlated within individuals (0.581, P = 0.005), but other Ig types did not. Results support the hypothesis that the tested protocols are an effective method for the non-invasive assessment of cattle immunology. The method could be used as part of animal health assessments, although further work is required to interpret the relationship between results and levels of infection and immunity.

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Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Author for correspondence: A. S. Cooke, E-mail: andrew.cooke@rothamsted.ac.uk

References

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Baklien, K and Brandtzaeg, P (1975) Comparative mapping of the local distribution of immunoglobulin-containing cells in ulcerative colitis and Crohn's disease of the colon. Clinical and Experimental Immunology 22, 197209.
Bath, GF and van Wyk, JA (2009) The Five Point Check© for targeted selective treatment of internal parasites in small ruminants. Small Ruminant Research 86, 613.
Bentounsi, B, Meradi, S and Cabaret, J (2012) Towards finding effective indicators (diarrhoea and anaemia scores and weight gains) for the implementation of targeted selective treatment against the gastro-intestinal nematodes in lambs in a steppic environment. Veterinary Parasitology 187, 275279.
Berghen, P et al. (1993) Evaluation of pepsinogen, gastrin and antibody response in diagnosing ostertagiasis. Veterinary Parasitology 46, 175195.
Bjerke, K, Brandtzaeg, P and Rognum, TO (1986) Distribution of immunoglobulin producing cells is different in normal human appendix and colon mucosa. Gut 27, 667674.
Blanchard, JL and Wescott, RB (1985) Enhancement of resistance of lambs to Haemonchus contortus by previous infection with Ostertagia circumcincta. American Journal of Veterinary Research 46, 21362140.
Bosco, A et al. (2014) The comparison of FLOTAC, FECPAK and McMaster techniques for nematode egg counts in cattle. Acta Parasitologica 59, 625628.
Capitini, LA, McClure, KE and Herd, RP (1990) Effect of environmental stimuli on pre-infective and infective stages of Haemonchus contortus in the Northern United States for the induction of hypobiosis. Veterinary Parasitology 35, 281293.
Charlier, J et al. (2011) Serum pepsinogen levels to monitor gastrointestinal nematode infections in cattle revisited. Research in Veterinary Science 90, 451456.
Charlier, J et al. (2014) Practices to optimise gastrointestinal nematode control on sheep, goat and cattle farms in Europe using targeted (selective) treatments. The Veterinary Record 175, 250255.
Claerebout, E and Vercruysse, J (2000) The immune response and the evaluation of acquired immunity against gastrointestinal nematodes in cattle: a review. Parasitology 120, 2542.
Coop, RL and Holmes, PH (1996) Nutrition and parasite interaction. International Journal for Parasitology 26, 951962.
Crabbé, PA and Heremans, JF (1968) The distribution of immunoglobulin-containing cells along the human gastrointestinal tract. Gastroenterology 54, 822825.
Cringoli, G et al. (2010) FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nature Protocols 5, 503515.
Dong, J et al. (2008) Emerging pathogens: challenges and successes of molecular diagnostics. The Journal of Molecular Diagnostics 10, 185197.
Fahey, JL and McKelvey, EM (1965) Quantitative determination of serum immunoglobulins in antibody-agar plates. Journal of Immunology 94, 8490.
Gisbert, JP et al. (2009) Fecal calprotectin and lactoferrin for the prediction of inflammatory bowel disease relapse. Inflammatory Bowel Diseases 15, 11901198.
Harrison, GBL et al. (2003) Characterization of a 35-kDa carbohydrate larval antigen (CarLA) from Trichostrongylus colubriformis; a potential target for host immunity. Parasite Immunology 25, 7986.
Hughes, DL, Hanna, REB and Symonds, HW (1981) Fasciola hepatica: IgG and IgA levels in the serum and bile of infected cattle. Experimental Parasitology 52, 271279.
Lamb, CA and Mansfield, JC (2011) Measurement of faecal calprotectin and lactoferrin in inflammatory bowel disease. Frontline Gastroenterology 2, 1318.
Lamm, ME (1988) The IgA mucosal immune system. American Journal of Kidney Diseases 12, 384387.
Lundberg, JO et al. (2005) Technology insight: calprotectin, lactoferrin and nitric oxide as novel markers of inflammatory bowel disease. Nature Reviews. Gastroenterology & Hepatology 2, 96102.
Macpherson, AJ et al. (2008) The immune geography of IgA induction and function. Mucosal Immunology 1, 1122.
Mazanec, MB et al. (1993) A three-tiered view of the role of IgA in mucosal defense. Immunology Today 14, 430435.
Miller, HRP (1987) Gastrointestinal mucus, a medium for survival and for elimination of parasitic nematodes and protozoa. Parasitology 94, S77S100.
Molina, JM et al. (1999) Cross-reactive antigens of Haemonchus contortus adult worms in Teladorsagia circumcincta infected goats. Veterinary Research 30, 393399.
Nagler-Anderson, C (2001) Man the barrier! Strategic defences in the intestinal mucosa. Nature Reviews Immunology 1, 5967.
Nawa, Y et al. (1994) Selective effector mechanisms for the expulsion of intestinal helminths. Parasite Immunology 16, 333338.
Negrão-Corrêa, D, Adams, LS and Bell, RG (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats. Journal of Immunology 157, 40374044.
Newkirk, MM et al. (2005) Elevated levels of IgM and IgA antibodies to Proteus mirabilis and IgM antibodies to Escherichia coli are associated with early rheumatoid factor (RF)-positive rheumatoid arthritis. Rheumatology 44, 14331441.
Nielsen, SS et al. (2000) Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. Preventive Veterinary Medicine 44, 17.
Onah, DN and Nawa, Y (2000) Mucosal immunity against parasitic gastrointestinal nematodes. Korean Journal of Parasitology 38, 209236.
Parker, AM et al. (2017) Bulk tank milk antibody ELISA as a biosecurity tool for detecting dairy herds with past exposure to Mycoplasma bovis. Journal of Dairy Science 100, 82968309.
Parkins, JJ and Holmes, PH (1989) Effects of gastrointestinal helminth parasites on ruminant nutrition. Nutrition Research Reviews 2, 227246.
Peters, IR et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs. Clinical and Diagnostic Laboratory Immunology 11, 841848.
Poppi, D, Sykes, A and Dynes, R (1990) The effect of endoparasitism on host nutrition – the implications for nutrient manipulation. Proceedings of the New Zealand Society of Animal Production 50, 237244.
Røseth, AG, Schmidt, PN and Fagerhol, MK (1999) Correlation between faecal excretion of indium-111-labelled granulocytes and calprotectin, a granulocyte marker protein, in patients with inflammatory bowel disease. Scandinavian Journal of Gastroenterology 34, 5054.
Ruma, J, Joydeb, G and Kinusk, D (2016) Antigenic cross-reactivity among Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis of goat. Iranian Journal of Parasitology 11, 542548.
Sansonetti, PJ (2004) War and peace at mucosal surfaces. Nature Reviews Immunology 4, 953964.
Sekiya, M, Zintl, A and Doherty, ML (2013) Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review. Irish Veterinary Journal 66, 14.
Shaw, RJ et al. (2012) Salivary IgA: a suitable measure of immunity to gastrointestinal nematodes in sheep. Veterinary Parasitology 186, 109117.
Smith, WD et al. (1985) Age immunity to Ostertagia circumcincta: comparison of the local immune responses of 4 1/2- and 10-month-old lambs. Journal of Comparative Pathology 95, 235245.
Stabel, JR, Wells, SJ and Wagner, BA (2002) Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne's disease in US dairy herds. Journal of Dairy Science 85, 525531.
Sykes, AR, Coop, RL and Angus, KW (1975) Experimental production of osteoporosis in growing lambs by continuous dosing with Trichostrongylus colubriformis larvae. Journal of Comparative Pathology 85, 549559.
Tomasi, TB (1970) Structure and function of mucosal antibodies. Annual Review of Medicine 21, 281298.
van Wyk, JA et al. (2006) Targeted selective treatment for worm management – How do we sell rational programs to farmers? Veterinary Parasitology 139, 336346.
Watt, KA et al. (2015) Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies. Ecology and Evolution 6, 5667.
Wedrychowicz, HZ, Maclean, JM and Holmes, PH (1985) Some observations on a possible role of lung and fecal IgA antibodies in immunity of rats to Nippostrongylus brasiliensis. Journal of Parasitology 71, 6269.
Wennerås, C et al. (1999) Intestinal immune responses in patients infected with enterotoxigenic Escherichia coli and in vaccines. Infection and Immunity 67, 62346241.
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