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Investigation of giardiasis in captive animals in zoological gardens with strain typing of assemblages in China

Published online by Cambridge University Press:  08 June 2021

Hua Liu ,
Bin Wang ,
Jianhai Yin ,
Zhongying Yuan ,
Yanyan Jiang ,
Jing Zhang ,
Jianping Cao Open the ORCID record for Jianping Cao [Opens in a new window] ,
Yujuan Shen Open the ORCID record for Yujuan Shen [Opens in a new window]  and
Hui Liu
Hua Liu*
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Bin Wang
Affiliation:
Department of Parasitology, Zunyi Medical University, Zunyi563000, China Guizhou Center for Disease Control and Prevention, Guiyan550000, China
Jianhai Yin
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Zhongying Yuan
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Yanyan Jiang
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Jing Zhang
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Jianping Cao
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Yujuan Shen*
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China
Hui Liu*
Affiliation:
Department of Parasitology, Zunyi Medical University, Zunyi563000, China
*
Authors for correspondence: Yujuan Shen, E-mail: shenyj@nipd.chinacdc.cn; Hui Liu, E-mail: liuhui6032@sina.com
Authors for correspondence: Yujuan Shen, E-mail: shenyj@nipd.chinacdc.cn; Hui Liu, E-mail: liuhui6032@sina.com
Authors for correspondence: Yujuan Shen, E-mail: shenyj@nipd.chinacdc.cn; Hui Liu, E-mail: liuhui6032@sina.com
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Abstract

Giardia duodenalis is a common zoonotic intestinal pathogen. It has been increasingly reported in humans and animals; however, genotyping information for G. duodenalis in captive animals is still limited. This study was conducted to assess the prevalence and multilocus genotyping of G. duodenalis in captive animals in zoological gardens in Shanghai, China. A total of 678 fresh fecal samples were randomly collected from captive animals including non-human primates (NHPs) (n = 190), herbivores (n = 190), carnivores (n = 151), birds (n = 138) and reptiles (n = 9) in a zoo and were examined for the presence of G. duodenalis using nested polymerase chain reaction (nested PCR). All G. duodenalis positive samples were assayed with PCR followed by sequencing at β-giardin (bg), glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) genes. In this study, 42 specimens (6.2%) were tested G. duodenalis-positive of the 678 fecal samples examined based on a single locus. A total of 30 (4.4%), 30 (4.4%) and 22 (3.2%) specimens were successfully amplified and sequenced at gdh, tpi and bg loci, respectively. Assemblages A and B were identified with assemblage B dominating in NHPs. Sequence analysis demonstrated that one, two and five new isolates were identified at bg, gdh and tpi loci. DNA sequences and new assemblage-subtypes of zoonotic G. duodenalis assemblages A and B were identified in the current study. Our data indicate the occurrence and molecular diversity of G. duodenalis and the potential zoonotic transmission in captive animals in China.

Keywords

Captive animalsGiardia duodenalismultilocus genotypingzoonotic transmission
Type
Research Article
Information
Parasitology , Volume 148 , Issue 11 , September 2021 , pp. 1360 - 1365
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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