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The invariant surface glycoprotein ISG75 gene family consists of two main groups in the Trypanozoon subgenus

Published online by Cambridge University Press:  04 September 2006

T. TRAN ,
F. CLAES ,
J. C. DUJARDIN  and
P. BUSCHER
T. TRAN
Affiliation:
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B-2000 Antwerp, Belgium Vrije Universiteit Brussel, Department of Biotechnology, Pleinlaan 2, B-1050 Brussels, Belgium
F. CLAES
Affiliation:
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B-2000 Antwerp, Belgium
J. C. DUJARDIN
Affiliation:
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B-2000 Antwerp, Belgium
P. BUSCHER
Affiliation:
Institute of Tropical Medicine, Department of Parasitology, Nationalestraat 155, B-2000 Antwerp, Belgium
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Abstract

In Trypanosoma brucei brucei, an invariant surface glycoprotein of molecular weight 75 kDa (ISG75) is uniformly distributed over the surface of a trypanosome and is specific for bloodstream-form parasites. For the other taxa of the Trypanozoon subgenus no data about this surface molecule are available. Therefore, we investigated the ISG75 in the genomes of several pathogenic Trypanozoon by Southern blot, PCR and RT-PCR and sequence analysisNucleotide sequence data reported in this paper are available in the GeneBank™, EMBL and DDBJ databases under the Accession numbers DQ200175-DQ200256.. This study reveals that (i) all members of the Trypanozoon subgenus, i.e. T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. evansi and T. equiperdum, harbour ISG75 as multiple gene copies with at least 4–16 copies per genome; (ii) ISG75 gDNA and cDNA sequences are distributed in 2 groups that share at least 75% and 77% identity respectively; (iii) sequences from both groups are transcribed in all species and subspecies of the Trypanozoon subgenus; (iv) the main differences between group I and group II are located in the variable region at the amino-terminus of the putative proteins; (v) however, all the sequences in both groups have some well-conserved features, such as the cysteine residues, an amino-terminal cleavable signal peptide, a single α-helix transmembrane domain and a cytoplasmic domain at the carboxy-terminus.

Keywords

Trypanosoma bruceiTrypanozooninvariant surface glycoprotein (ISG75)molecular characterization
Type
Research Article
Information
Parasitology , Volume 133 , Issue 5 , November 2006 , pp. 613 - 621
Copyright
2006 Cambridge University Press

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