Skip to main content Accessibility help
×
Home

Infectivity, antigenicity and host responses to isolates of the genus Trichinella

  • F. Bolas-Fernandez (a1) and D. Wakelin (a1)

Summary

Comparisons were made of the infectivity and antigenicity of 4 Trichinella spiralis isolates (S, D, Y, W), of quite different geographical origins, and T. pseudospiralis (P) in rapid- and slow-responder inbred mice. Infectivity was measured by the Index of Reproductive Capacity (ICR) expressed as the ratio between the number of muscle larvae recovered on day 30 post-infection (p.i.) and the numbers of larvae given at infection. Antigen recognition was measured by the degree of proliferation of mesenteric lymph node cells (MLNC) to in vitro stimulation with crude muscle larvae antigen (CMLA) and by the total antibody responses to CMLA at day 25 p.i. as measured by ELISA. Regarding infectivity the isolates fell into two groups, high infectivity (S, D and Y) and low infectivity (W and P). Analysis of CMLA, detergent-stripped (CTAB) and I-labelled surface larval proteins was made by SDS—PAGE under reducing conditions. Differences in antigen profiles were seen in all antigen preparations, being most noticeable in CTAB and 125I-labelled proteins from W and P isolates. Antigen recognition by polyclonal infection-derived antisera and by monoclonal antibodies raised against the T. spiralis London strain (L) was studied in the W (Arctic) and S (Spanish) isolates. Polyclonal antisera recognized different antigens in the S and W isolates, as did the monoclonal antibody, although recognition was more restricted. Neither antibody recognized a 64 kDa band in the W isolate which was clearly visible in the others tested.

Copyright

References

Hide All
Almond, N. M., Mclaren, D. J. & Parkhouse, R. M. E. (1986). A comparison of the surface and secretions of Trichinella pseudospiralis and T. spiralis. Parasitology 93, 163–76.
Arriaga, C., Muniz, E., Morilla, A. & Ortega Pierres, M. G. (1989). Evaluation of purified surface stichosomal components of Trichinella spiralis muscle larvae for diagnosis of swine trichinellosis. In Trichinellosis (ed. Tanner, Ch., Martinez, A. R. & Bolas, F.) pp. 182–7. Madrid: Consejo Superior de Investigaciones Cientificas.
Bolas-Fernandez, F. & Wakelin, D. (1980). Infectivity of Trichinella isolates in mice is determined by host immune responsiveness. Parasitology 99, 83–8.
Dame, J. B., Murrell, K. D., Worley, D. E. & Schad, G. A. (1987). Trichinella spiralis: Genetic evidence for synanthropic subspecies in sylvatic hosts. Experimental Parasitology 64, 195203.
Dick, T. A. (1983). Species and intraspecific variation. In Trichinella and Trichinellosis (ed. Campbell, W. C.), pp. 3173. New York: Plenum Press.
Dick, T. A., Currans, J. & Klassen, G. (1985). Genetic and molecular biology of Trichinella. In Trichinellosis (ed. Kim, W. K.) pp. 118128. New York: State University of New York Press.
Gamble, H. R. & Murrell, K. D. (1986). Conservation of diagnostic antigen epitopes among biologically diverse isolates of Trichinella spiralis. Journal of Parasitology 72, 921–5.
Grencis, R. K., Crawford, C., Pritchard, D. I., Behnke, J. M. & Wakelin, D. (1986). Immunization of mice with surface antigens from the muscle larvae of Trichinella spiralis. Parasite Immunology 8, 587–96.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 277, 680–5.
Lee, T. D., Grencis, R. K. & Wakelin, D. (1982). Specific cross-immunity between Trichinella spiralis and Trichuris muris: immunization with heterologous infections and antigens and transfer of immunity with heterologous immune mesenteric lymph node cells. Parasitology 84, 381–9.
Martinez-Fernandez, A. R., Gomez-Barrio, A., Armas-Serra, C. & Albarran, E. (1989). Trichinella spiralis GM-I x T. pseudospiralis garkavi: generational behavior. In Trichinellosis (ed. Tanner, Ch., Martinez, A. R. & Bolas, F.) pp. 8993. Madrid: Consejo Superior de Investigaciones Cientificas.
Phillip, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of a parasitic nematode. Nature, London 287, 538–40.
Rappaport, I. (1943 a). A comparison of three strains on Trichinella spiralis. I. Pathogenicity and extent of larval development in the musculature. American Journal of Tropical Medicine 23, 343–50.
Rappaport, I. (1943 b). A comparison of three strains of Trichinella spiralis. II. Longevity and sex ratio of adults in the intestine and rapidity of larval development in the musculature. American Journal of Tropical Medicine 23, 351–62.
Schad, G. A., Duffy, C. H., Leiby, D. A., Murrell, K. D. & Zirkle, E. W. (1987). Trichinella spiralis in an agricultural ecosystem: transmission under natural and experimentally modified farm conditions. Journal of Parasitology 73, 95102
Silberstein, D. S. & Despommier, D. D. (1984). Antigens from Trichinella spiralis that induce a protective response in the mouse. Journal of Immunology 132, 898904.
Wakelin, D. & Lloyd, M. (1976). Immunity to primary and challenge infections of Trichinella spiralis in mice: a re-examination of conventional parameters. Parasitology 72, 173–82.
Wakelin, D., Donachie, A. M. & Grencis, R. K. (1985). Genetic control of immunity to Trichinella spiralis in mice: capacity of cells from slow responder mice to transfer immunity in syngeneic and F1 hybrid recipients. Immunology 86, 203–11.
Wassom, D. L., Dougherty, D. A. & Dick, T. A. (1988). Trichinella spiralis infections in inbred mice: immunologically specific responses induced by different Trichinella isolates. Journal of Parasitology 72, 283–7.
Zarlenga, D. S. & Gamble, R. H. (1989). Identification and characterization of a cDNA clone expressing an excretory- secretory antigen from Trichinella spiralis muscle larvae. In Trichinellosis, (ed. Tanner, Ch., Martinez, A. R. & Bolas, F.) pp. 2834. Madrid: Consejo Superior de Investigaciones Cientificas.

Keywords

Infectivity, antigenicity and host responses to isolates of the genus Trichinella

  • F. Bolas-Fernandez (a1) and D. Wakelin (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed