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Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library

  • A. VILLA-MANCERA (a1), H. QUIROZ-ROMERO (a1), D. CORREA (a2), F. IBARRA (a1), M. REYES-PÉREZ (a1), H. REYES-VIVAS (a3), G. LÓPEZ-VELÁZQUEZ (a3), K. GAZARIAN (a4), T. GAZARIAN (a4) and R. A. ALONSO (a5)...

Summary

An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1×1014 phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47·61% and 33·91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58·92 to 82·11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica.

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Corresponding author

*Corresponding author: Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México, D.F. 04510. Tel: +52 56225898. Fax: +52 56225898. E-mail: hquiroz@servidor.unam.mx

References

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