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The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients

  • E. H. Carter (a1), A. Suhrbier (a1), P. J. A. Beckers (a2) and R. E. Sinden (a1)

Summary

Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0·45% become mature ookinetes. A single-step gradient centrifugation method on 12·5% Nycodenz® enriches both gametocytes and ookinetes.

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*Reprint requests to Dr R. E. Sinden.

References

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The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients

  • E. H. Carter (a1), A. Suhrbier (a1), P. J. A. Beckers (a2) and R. E. Sinden (a1)

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