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Identification of midgut trypanolysin and trypanoagglutinin in Glossina palpalis sspp. (Diptera: Glossinidae)

Published online by Cambridge University Press:  06 April 2009

J. K. Stiles ,
G. A. Ingram ,
K. R. Wallbanks ,
D. H. Molyneux ,
I. Maudlin  and
S. Welburn
J. K. Stiles
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
G. A. Ingram
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
K. R. Wallbanks
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
D. H. Molyneux
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
I. Maudlin
Affiliation:
Tsetse Research Laboratory, School of Veterinary Medicine, University of Bristol, Bristol BS18 7DU
S. Welburn
Affiliation:
Tsetse Research Laboratory, School of Veterinary Medicine, University of Bristol, Bristol BS18 7DU
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Summary

A midgut trypanolysin and an agglutinin from Glossina palpalis subspecies were isolated and partially characterized using anion-exchange chromatography and polyacrylamide gel electrophoresis. FPLC fractions of midgut extracts of Glossina palpalis palpalis caused agglutination and lysis of two trypanosome species (Trypanosoma congolense and Trypanosoma brucei brucei). although Glossina palpalis gambiensis caused only agglutination. The trypanolysin and agglutinin were active only in the posterior midguts, were heat labile above 50%C, had a periodic cycle of ‘activity’ in response to bloodmeal intake and were not affected by protease inhibitors or trypsin but were inactivated by pronase. The lytic substance contained two proteins with approximate molecular weights (Mr) of 12000 and 10000 Da respectively. The agglutinin had an approximate Mr of 67000 Da. Gamma-irradiation of the two subspecies caused a temporary inhibition of trypanolytic and agglutinin activities in midgut extracts.

Keywords

Glossinamidguttrypanolysintrypanoagglutinin
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 369 - 376
Copyright
Copyright © Cambridge University Press 1990

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