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Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius

  • ZE-HONG LI (a1), JIA-YU WAN (a2), GUI-QIN WANG (a1), FU-GUANG ZHAO (a1) and JI-HONG WEN (a3)...

Summary

The present study was designated to ascertain the anthelmintic activity of the rhizomes of Paris polyphylla and to isolate and characterize the active constituents. The methanol extract from rhizomes of P. polyphylla showed significant anthelmintic activity against Dactylogyrus intermedius with the median effective concentration (EC50) 22·5 mg L−1. Based on this finding, the methanol extract was fractionated by silica gel column chromatography in a bioassay-guided fractionation yielding 2 bioactive compounds, the structures of these compounds were elucidated as formosanin C and polyphyllin VII. The in vivo tests revealed that formosanin C and polyphyllin VII were significantly effective against D. intermedius with EC50 values of 0·6 and 1·2 mg L−1, respectively. The acute toxicities (LC50) of formosanin C and polyphyllin VII for grass carp were 2·8 and 2·9 mg L−1, respectively. The overall results provide important information for the potential application of formosanin C and polyphyllin VII in the therapy of serious infection caused by D. intermedius.

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Corresponding author

*Corresponding author. Obstetrics and Gynecology Department, The First Hospital of JiLin University, Changchun 130021, China. Fax: +86431-88782182. E-mail: jihong.wen@yahoo.com.cn

References

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Bourgaud, F., Gravot, A., Milesi, E. and Gontier, E. (2001). Production of plant secondary metabolites: an historical perspective. Plant Science 161, 839851.
Buchmann, K., Slotved, H. C. and Dana, D. (1993). Epidemiology of gill parasite infections in Cypirnus carpio in Indonesia and possible control methods. Aquaculture 118, 921.
Chiang, H. C., Wang, J. J. and Wu, R. T. (1992). Immunomodulating effects of the hydrolysis products of formosanin C and beta-ecdysone from formosana Hayata. Anticancer Research 12, 14751478.
Chen, C. X. and Zhou, J. (1981). Studies on the saponin components of plants in Yunnan: V. Steroid glycosides and β-ecdysone of Paris polyphylla SM. var. Yunnanensis (FR.) H-M. Acta Botanica Yunnanica 3, 8993.
Chen, C. X., Zhang, Y. T. and Zhou, J. (1983). Studies on the saponin components of plants in Yunnan: VI. Steroid glycosides of Paris polyphylla SM. var. Yunnanensis (FR.) H-M (2). Acta Botanica Yunnanica 5, 9197.
Desagher, S. and Martinou, J. C. (2000). Mitochondria as the central control point of apoptosis. Trends in Cell Biology 10, 369–77.
Dove, A. and Ernst, I. (1998). Concurrent invaders – four exotic species of Monogenea now established on exotic freshwater fishes in Australia. International Journal for Parasitology 28, 17551764.
Huang, C. Y., Chou, K. Y. and Chang, H. C. (1990). Metabolism of formosanin-C by cytochrome P-450. European Journal of Pharmacology 183, 1360.
Huang, Q. A., Qiao, F., Lu, J. and Lin, R. C. (2007). Studies on the separation and quantity determination of saponins of Rhizoma Paridis. Chinese Pharmaceutical Affairs 21, 591593.
İsmail, K. I. R. and Selda, T. Ö. (2007). Helminth infections in common carp, Cyprinus Carpio L., 1958 (Cyprinidae) from Kovada Lake (Turkey). Türkiye Parazitoloji Dergisi 31, 232236 (in German with English abstract).
Lee, J. C., Su, C. L., Chen, L. L. and Won, S. J. (2009). Formosanin C-induced apoptosis requires activation of caspase-2 and change of mitochondrial membrane potential. Cancer 100, 503513.
Li, X. L., Yao, J. Y., Zhou, Z. M., Shen, J. Y., Ru, H. S. and Liu, X. L. (2011). Activity of the chelerythrine, a quaternary benzo[c]phenanthridine alkaloid from Chelidonium majus L. on Dactylogyrus intermedius. Parasitology Research 109, 247252.
Liu, Y. T., Wang, F., Wang, G. X., Han, J., Wang, Y. and Wang, Y. H. (2010). In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitology Research 106, 12331239.
Marshall, C. J. (1999). Use of Supaverm® for the treatment of monogenean infestation in koi carp (Cyprinus carpio). Fish Veterinary Journal 4, 3337.
Reed, P. A., Francis, F. R. and Klinger, R. C. (2009). FA28/FA033: Monogenean parasites of fish. EDIS-Electronic Data Information Source-UF/IFAS Extension. University of Florida. http://edis.ifas.ufl.edu/FA033. 17 May 2009.
Saraste, A. (1999). Morphologic criteria and detection of apoptosis. Herz 24, 189–95.
Schmahl, G. and Mehlhorn, H. (1985). Treatment of fish parasites: 1. Praziquantel effective against Monogenea (Dactylogyrus vastator, Dactylogyrus extensus, Diplozoon paradoxum). Zeitschrift für Parasitenkunde 71, 727737.
Schmahl, G. and Mehlhorn, H. (1988). Treatment of fish parasites: 4. Effects of symtriazinone (toltrazuril) on monogenea. Parasitology Research 75, 132143.
Tagboto, S. and Townson, S. (2001). Antiparasitic properties of medicinal plants and other naturally occurring products. Advances in Parasitology 50, 199295.
Topić, P. N., Hacmanjek, M. and Teskeredžić, E. (2001). Health status of rudd (Scardinius erythrophthalmus hesperidicus H.) in Lake Vrana on the Island of Cres, Croatia. Journal of Applied Ichthyology 17, 4345.
Treves-Brown, K. M. (1999). Availability of medicines for fish. Fish Veterinary Journal 4, 4055.
Wang, G. X., Chen, C., Cheng, L. A. and Zhang, X. (2006). Study on killing activities of 22 plants and 6 plant compounds to the Dactylogyrus. Acta Botanica Boreal-Occident Sinica 26, 25672573. (in Chinese with English abstract).
Wang, G. X., Zhou, Z., Cheng, C., Yao, J. Y. and Yang, Z. W. (2008). Osthol and isopimpinellin from Fructus cnidii for the control of Dactylogyrus intermedius in Carassius auratus. Veterinary Parasitology 158, 144151.
Wu, R. T., Chang, H. C., Fu, W. C., Chen, K. Y., Chung, Y. M. and Horng, L. Y. (1990). Formosanin-C, an immunomodulator with antitumor activity. International Journal of Immunopharmacology 12, 777–86.
Yao, J. Y., Li, X. L., Shen, J. Y., Pan, X. Y., Hao, G. J., Xu, Y., Yin, W. L., Ru, H. S. and Liu, X. L. (2011). Isolation of bioactive components from Chelidonium majus L. with activity against Trichodina sp. Aquaculture 318, 235238.

Keywords

Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius

  • ZE-HONG LI (a1), JIA-YU WAN (a2), GUI-QIN WANG (a1), FU-GUANG ZHAO (a1) and JI-HONG WEN (a3)...

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