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Hypoxanthine: a low molecular weight factor essential for growth of erythrocytic Plasmodium falciparum in a serum-free medium

  • H. Asahi (a1), T. Kanazawa (a1), Y. Kajihara (a2), K. Takahashi (a2) and T. Takahashi (a3)...


A low molecular weight factor in a basal medium essential for erythrocytic Plasmodium falciparum development in a serum-free medium using a cell growth-promoting factor derived from adult bovine serum was detected. The factor was hypoxanthine. The optimal hypoxanthine concentration for parasite growth was between 15 and 120 μM. The contribution of hypoxanthine to increased parasite growth was clearly evident in cultures on day 4. Among various low molecular weight supplements tested, adenine, adenosine, AMP, ATP, cyclic AMP, guanine, guanosine, inosine, inosine mono-phosphate, xanthine, NAD, NADH, NADP, NADPH and deoxyguanosine triphosphate showed a similar effect to that of hypoxanthine in the serum-free culture system. On the other hand, the addition of uric acid, FAD, thymidine, uridine, orotic acid, deoxythymidine triphosphate, deoxycytidine triphosphate, deoxyadenosine triphosphate, ribose-1-phosphate, or ethanolamine was not beneficial to the parasite growth. The results presented here will not only be of practical value, but will provide important information about the developmental requirements of the parasite.


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Asahi, H. & Kanazawa, T. (1994). Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growth-promoting factor. Parasitology 109, 397401.
Chanock, R. M. & Sabin, A. B. (1953). The hemagglutinin of St. Louis encephalitis virus. I. Recovery of stable hemagglutinin from the brains of infected mice. Journal of Immunology 70, 271–85.
Divo, A. A. & Jensen, J. B. (1982). Studies on serum requirements for the cultivation of Plasmodium falciparum. 2. Medium enrichment. Bulletin of the World Health Organization 60, 571–5.
Divo, A. A., Vande, Waa J. A., Campbell, J. R. & Jensen, J. B. (1985). Isolation and cultivation of Plasmodium falciparum using adult bovine serum. Journal of Parasitology 71, 504–9.
Elabbadi, N., Ancelin, M. L. & Vial, H. J. (1992). Use of radioactive ethanolamine incorporation into phospholipids to assess in vitro antimalarial activity by the semiautomated microdilution technique. Antimicrobial Agents and Chemotherapy 36, 50–5.
Gero, A. M. & O'Sullivan, W. J. (1990). Purines and pyrimidines in malarial parasites. Blood Cells 16, 467–84.
Grellier, P., Rigomier, D., Clavey, V., Fruchart, J.-C. & Schrevel, J. (1991). Lipid traffic between high density lipoproteins and Plasmodium falciparum-infected red blood cells. Journal of Cell Biology 112, 267–77.
Gutteridge, W. E. & Trigg, P. I. (1970). Incorporation of radioactive precursors into DNA and RNA of Plasmodium knowlesi in vitro. Journal of Protozoology 17, 8996.
Japanese Patent (1985). Number Syo60–145088.
Kudo, T., Morishita, R., Suzuki, (Seno) R. & Tachibana, T. (1987). A great improvement of fusion efficiency in mouse B cell hybridoma production by use of the new culture medium, GIT. Tohoku Journal of Experimental Medicine 153, 5566.
Kugler, G. (1978). A column chromatographic method for determination of plasma and erythrocyte levels of inosine and hypoxanthine. Analytical Biochemistry 90, 204–10.
Lingnau, A., Margos, G., Maier, W. A. & Seitz, H. M. (1993). Serum-free cultivation of Plasmodium falciparum gametocytes in vitro. Parasitology Research 79, 378–84.
Ofulla, A. V. O., Okoye, V. C. N., Khan, B., Githure, J. I., Roberts, C. R., Johnson, A. J. & Martin, S. K. (1993). Cultivation of Plasmodium falciparum parasites in a serum-free medium. American Journal of Tropical Medicine and Hygiene 49, 335–40.
Reyes, P., Rathod, P. K., Sanchez, D. J., Mrema, J. E. K., Rieckmann, K. H. & Heidrich, H-G. (1982). Enzymes of purine and pyrimidine metabolism from the human malaria parasite, Plasmodium falciparum. Molecular and Biochemical Parasitology 5, 275–90.
Sherman, I. W. (1977). Transport of amino acids and nucleic acid precursors in malarial parasites. Bulletin of the World Health Organization 55, 211–25.
Sherman, I. W. (1979). Biochemistry of Plasmodium (malarial parasites). Microbiological Reviews 43, 453–95.
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.
Webster, H. K. & Whaun, J. M. (1981). Purine metabolism during continuous erythrocytes culture of human malaria parasites (P. falciparum). Progress in Clinical and Biological Research 55, 557–73.
Zolg, J. W., Macleod, A. J., Dickson, I.H. & Scaife, J. G. (1982). Plasmodium falciparum: modifications of the in vitro culture conditions improving parasite yields. Journal of Parasitology 68, 1072–80.


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Hypoxanthine: a low molecular weight factor essential for growth of erythrocytic Plasmodium falciparum in a serum-free medium

  • H. Asahi (a1), T. Kanazawa (a1), Y. Kajihara (a2), K. Takahashi (a2) and T. Takahashi (a3)...


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