Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-06-22T11:14:11.607Z Has data issue: false hasContentIssue false
  • English
  • Français

A hybrid gene to express protein epitopes from both sporozoite and merozoite surface antigens of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

A. A. Holder ,
M. J. Lockyer  and
G. W. Hardy
A. A. Holder
Affiliation:
Departments ofWellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
M. J. Lockyer
Affiliation:
Departments ofWellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
G. W. Hardy
Affiliation:
Departments of Molecular Biology and Medicinal Chemistry
Get access Rights & Permissions [Opens in a new window]

Summary

The DNA coding for parts of the repetitive amino acid sequence of the Plasmodium falciparum circumsporozoite protein has been spliced to a sequence encoding part of the precursor to the major merozoite surface antigens, to produce a hybrid gene. Expression in Escherichia coli produces a protein with antigenic determinants from both malaria proteins. Antibodies raised against the expressed material react with both a peptide derived from the circumsporozoite repeat sequence, and the merozoite surface molecule. Hybrid molecules of this type may be the basis of a malaria vaccine.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 3 , December 1988 , pp. 373 - 382
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Atherton, E., Gait, M. J., Sheppard, R. C. & Williams, B. J. (1979). The polyamide method of solid phase peptide and oligonucleotide synthesis. Bioorganic Chemistry 8, 351–70.Google Scholar
Ballou, W. R., Sherwood, J. A., Neva, F. A., Gordon, D. M., Wirtz, R. A., Wasserman, G. F., Diggs, C. L., Hoffman, S. L., Hollingdale, M. R., Hockmeyer, W. T., Schneider, I., Young, J. F., Reeve, P. & Chulay, J. D. (1987). Safety and efficacy of a recombinant DNA Plasmodium falciparum sporozoite vaccine. Lancet 1, 1277–81.CrossRefGoogle ScholarPubMed
Barany, G., Kneib-Cordonier, N. & Mullen, D. G. (1987). Solid phase peptide synthesis: a silver anniversary report. International Journal of Peptide and Protein Research 30, 705–39.CrossRefGoogle Scholar
Chen, E. Y. & Seeburg, P. H. (1985). Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4, 165–70.Google Scholar
Cheung, A., Leban, J., Shaw, A. R., Merkli, B., Stocker, J., Chizzolini, C., Sander, C. & Perrin, L. H. (1986). Immunization with synthetic peptides of a Plasmodium falciparum surface antigen induces antimerozoite antibodies. Proceedings of the National Academy of Sciences, USA 83, 8328–32.CrossRefGoogle ScholarPubMed
Hall, R., Hyde, J. E., Goman, M., Simmons, D. L., Hope, I. A., Mackay, M., Scaife, J. G., Merkli, B., Richle, R. & Stocker, J. (1984). Major surface antigen gene of a human malaria parasite cloned and expressed in bacteria. Nature, London 311, 379–82.CrossRefGoogle ScholarPubMed
Herrington, D. A., Clyde, D. F., Losonsky, G., Cortesia, M., Murphy, J. R., Davis, J., Baqar, S., Felix, A. M., Heimer, E. P., Gillessen, D., Nardin, E., Nussenzweig, R. S., Nussenzweig, V., Hollingdale, M. R. & Levine, M. M. (1987). Safety and immunogenicity in man of a synthetic peptide malaria vaccine against Plasmodium falciparum sporozoites. Nature, London 328, 257–9.CrossRefGoogle ScholarPubMed
Holder, A. A. (1988). The precursor to major merozoite surface antigens: structure and role in immunity. Progress in Allergy 41, 72–97.Google Scholar
Holder, A. A., Freeman, R. R. & Nicholls, S. C. (1988). Immunization against Plasmodium falciparum with recombinant polypeptides produced in Escherichia coli. Parasite Immunology (in the Press).Google Scholar
Holder, A. A., Lockyer, M. J., Odink, K. G., Sandhu, J. S., Riveros-Moreno, V., Nicholls, S. C., Hillman, Y., Davey, L. S., Tizard, M. L. V., Schwarz, R. T. & Freeman, R. R. (1985). Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites. Nature, London 317, 270–3.CrossRefGoogle Scholar
Holder, A. A., Sandhu, J. S., Hillman, Y., Davey, L. S., Nicholls, S. C., Cooper, H. & Lockyer, M. J. (1987). Processing of the precursor to the major merozoite surface antigens of Plasmodium falciparum. Parasitology 94, 199–208.CrossRefGoogle Scholar
Lockyer, M. J. & Schwarz, R. T. (1987). Strain variation in the circumsporozoite protein gene of Plasmodium falciparum. Molecular and Biochemical Parasitology 22, 101–8.CrossRefGoogle ScholarPubMed
Lockyer, M. J. & Holder, A. A. (1988). Malaria. In Vaccination Strategies of Tropical Diseases (ed. Liew, F. Y.). Boca Raton: CRC Press Inc. (in the Press).Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning. A Laboratory Manual. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
Miller, L. H., Howard, R. J., Carter, R., Good, M. F., Nussenzweig, V. & Nussenzweig, R. S. (1986). Research towards malaria vaccines. Science 234, 1349–56.Google Scholar
Odink, K. G., Lockyer, M. J., Nicholls, S. C., Hillman, Y., Freeman, R. R. & Holder, A. A. (1984). Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites. FEBS Letters 173, 108–12.CrossRefGoogle Scholar
Patarroyo, M. E., Romero, P., Torres, M. L., Clavijo, P., Moreno, A., Martinez, A., Rodriguez, R., Guzman, F. & Cabezas, E. (1987). Induction of protective immunity against experimental infection with malaria using synthetic peptides. Nature, London 328, 629–32.CrossRefGoogle ScholarPubMed
Patarroyo, M. E., Amador, R., Clavijo, P., Moreno, A., Guzman, F., Romero, P., Tascon, R., Franco, A., Murillo, L. A., Ponton, G. & Trujillo, G. (1988). A synthetic vaccine protects humans against challenge with asexual blood stages of Plasmodium falciparum malaria. Nature, London 332, 158–61.CrossRefGoogle ScholarPubMed
Perrin, L. H., Merkli, B., Loche, M., Chizzolini, C., Smart, J. & Richle, R. (1984). Antimalarial immunity in Saimiri monkeys. Immunization with surface components of asexual blood stages. Journal of Experimental Medicine 160, 441–51.Google Scholar
Siddiqui, W. A., Tam, L. Q., Kramer, K. J., Hui, G. S. N., Case, S. E., Yamaga, K. M., Chang, S. P., Chan, E. B. T. & Kan, S-C. (1987). Merozoite surface coat precursor protein completely protects Aotus monkeys against Plasmodium falciparum malaria. Proceedings of the National Academy of Sciences, USA 84, 3014–18.CrossRefGoogle ScholarPubMed