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High-yield amplification of Cryptosporidium parvum in interferon γ receptor knockout mice

  • J. von OETTINGEN (a1) (a2), M. NATH-CHOWDHURY (a1), B. J. WARD (a1), A. C. RODLOFF (a2), M. J. ARROWOOD (a3) and M. NDAO (a1)...

Summary

To date, large-scale production of Cryptosporidium parvum oocysts has only been achieved by amplification in neonatal calves and sheep. Many laboratories currently depend on supplies from external sources and store oocysts for prolonged periods which results in progressive loss of viability. Six to 8-week-old interferon γ receptor knockout (IFNγR-KO) mice on a C57BL/6 background were inoculated by gavage (2000 oocysts/animal). Fecal pellets were collected daily from 7 days post-infection (p.i.) up to 2 weeks p.i. Intestinal oocyst yield was assessed at days 11, 12 and 14 p.i. by homogenization of intestinal tissues. Ether extraction and one or more NaCl flotations were used to purify oocysts. Total recoveries averaged 2·6×106 oocysts/mouse from fecal material and 3·8×107 oocysts/mouse from intestinal tissues. Overall, 2·3×109 purified oocysts were obtained from 60 mice. Recovered oocysts were capable of sporulation and were shown to be infectious both in vitro and in vivo. Oocyst amplification was achieved in only 11–14 days with minimal expense. The simplicity of this method presents a practical alternative for the routine passage, maintenance and storage of C. parvum in biomedical laboratories.

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Corresponding author

*Corresponding author: Research Institute of the McGill University Heath Centre, Montreal General Hospital Research Institute, Room R3-137. 1650 Cedar Ave, Montreal, Quebec, CanadaH3G 1A4. Tel: +1 514 934 8347. E-mail: momar.ndao@mail.mcgill.ca

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Keywords

High-yield amplification of Cryptosporidium parvum in interferon γ receptor knockout mice

  • J. von OETTINGEN (a1) (a2), M. NATH-CHOWDHURY (a1), B. J. WARD (a1), A. C. RODLOFF (a2), M. J. ARROWOOD (a3) and M. NDAO (a1)...

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