Skip to main content Accessibility help
×
Home

Extracellular matrix alterations in experimental murine Leishmania (L.) amazonensis infection

  • A. L. ABREU-SILVA (a1) (a2), K. S. CALABRESE (a2), R. A. MORTARA (a3), R. C. TEDESCO (a4) (a5), F. O. CARDOSO (a2), L. O. P. CARVALHO (a2) and S. C. GONÇALVES DA COSTA (a2)...

Abstract

Here we describe extracellular matrix alterations in footpad lesions and draining lymph nodes caused by Leishmania (L.) amazonensis in mouse strains with distinct susceptibilities to this parasite: BALB/c (susceptible), C57BL/6 (intermediate), and DBA/2 (resistant). Changes in ECM were observed mainly in BALB/c mice that, in general, presented tissue damage associated with high parasite burden. Under polarized light, Sirius Red revealed type I collagen that was predominant in the primary lesion in all strains studied at the early phase of infection, but gradually decreased and was replaced by abundant type III collagen fibres in chronic phase lesions. The presence of type III collagen seemed to provide support to inflammatory cells, mainly vacuolated and parasitized macrophages. Laminin expression was not altered during infection by L. (L.) amazonensis in any of the mouse strains studied. Furthermore, the decreased fibronectin expression, in all strains, in areas where amastigotes have been found, indicated that this decline was also not related to the genetic background.

Copyright

Corresponding author

Departamento Protozoologia, Laboratório de Imunomodulação, Fundação Oswaldo Cruz, Avenida Brasil, 4365-Pavilhão Carlos Chagas 3° andar, Manguinhos, RJ, Brazil, CEP 21045-900. Tel/Fax: +55 21 2560 6572. E-mail: calabresekatia@hotmail.com

References

Hide All

REFERENCES

ABREU-SILVA, A. L. (2003). Estudo sobre a visceralização da Leishmania (Leishmania) amazonensis (Kinetoplastida, Trypanosomatidae) no modelo murino. Thesis, Instituto Oswaldo Cruz, Rio de Janeiro.
AL ADNANI, M. S. (1985). Concomitant immunohistochemical localization of fibronectin and collagen in schistosome granulomata. Journal of Pathology 147, 7785.
BANDYOPADHYAY, K., SUDIPAN, K., GHOSH, A. & PIJUSK, K. (2001). Role of 67 kDa cell surface binding protein of Leishmania donovani in pathogenesis. Journal of Biochemistry 130, 141148.
BANDYOPADHYAY, K., SUDIPAN, K., GHOSH, A. & PIJUSK, K. (2002). High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences. European Journal of Biochemistry 269, 16221629.
BARRAL, A., PEDRAL-SAMPAIO, D., GRIMALDI Jr., G., MOMEN, H., McMAHON-PRATT, D., RIBEIRO DE JESUS, A., ALMEIDA, R., BADARO, R., BARRAL-NETO, M., CARVALHO, E. M. & JOHNSON Jr., W. D. (1991). Leishmaniasis in Bahia: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease. American Journal Tropical Medicine and Hygiene 44, 536546.
BECKER, K., HUNTER, I. & ENGEL, J. (1990). Structure and function of laminin: anatomy of a multidomain glycoprotein. FASEB Journal 4, 148160.
CALABRESE, K. S. & GONÇALVES DA COSTA, S. C. (1992). Enhancement of Leishmania amazonensis infection in BCG non-responder mice by BCG-antigen specific vaccine. Memórias do Instituto Oswaldo Cruz 87, 4956.
CUPOLILO, S. N. M., SOUZA, C. S. F., ABREU-SILVA, A. L., CALABRESE, K. S. & GONÇALVES DA COSTA, S. C. (2003). Biological behavior of Leishmania (L.) amazonensis isolated from a human diffuse cutaneous leishmaniasis in inbred strains of mice. Histology and Histopathology 18, 10591065.
GHOSH, A., BANDYOPADHYAY, K., KOLE, L. & PIJUSK, K. (1999). Isolation of a laminin-binding protein from the protozoan parasite Leishmania donovani that may cell adhesion. Biochemistry Journal 337, 551558.
GHOSH, A., KOLE, L., BANDYOPADHYAY, K., SARKAR, K. & PIJUSK, K. (1996). Evidence of laminin binding protein on the surface of Leishmania donovani. Biochemical and Biophysical Research Communications 226, 101106.
HANDMAN, E. (2001). Leishmaniasis: current status of vaccine development. Clinical Microbiological Review 14, 229243.
HODGKINSON, V. H., HERMAN, R. & SEMPREVIVO, L. (1980). Leishmania donovani: correlation among assays of amastigote viability. Experimental Parasitology 50, 397408.
LEITE, V. H. & CROFT, S. L. (1996). Hepatic extracellular matrix in BALB/c mice infected with Leishmania donovani. International Journal of Experimental Pathology 77, 181190.
LIRA, R., ROSALES-ENCINA, J. L. & ARGUELOS, C. (1997). Leishmania mexicana: binding of promastigotes to type I collagen. Experimental Parasitology 85, 149157.
McGWIRE, B. S., CHANG, K. P. & ENGMAN, D. M. (2003). Migration through the extracellular matrix by the parasitic protozoan Leishmania is enhanced by surface metalloprotease gp63. Infection and Immunity 71, 10081010.
MONTES, G. S. (1996). Structural biology of the fibers of the collagenous and elastic systems. Cell Biology International 20, 1527.
PROCTOR, R. A. (1987). Fibronectin: an enhancer of phagocyte function. Review of Infectious Disease 9, S412S419.
RHOADS, M. L. & FETTERER, R. H. (1996). Extracellular matrix degradation by Haemonchus contortus. Journal of Parasitology 82, 379383.
RODGERS, R. J. & IRVING-RODGERS, H. F. (2002). Extracellular matrix of the bovine ovarian membrana granulosa. Molecular and Cellular Endocrinology 191, 5764.
VANNIER-SANTOS, M. A., SARAIVA, E. M., MARTINY, A., NEVES, A. & DE SOUZA, W. (1992). Fibronectin shedding by Leishmania may influence the parasite–macrophage interaction. European Journal of Cell Biology 59, 389397.
WYLLER, D. J. (1987). Fibronectin in parasitic diseases. Review of Infectious Disease 4, S391S399.
WYLLER, D. J., SYPEC, J. P. & McDONALD, J. A. (1985). In vitro parasite–monocyte interactions in human leishmaniasis: possible role of fibronectin in parasite attachment. Infection and Immunity 49, 305311.
YAMADA, K. M. & KEMLER, R. (2002). Cell to cell contact and extracellular matrix. Current Opinion in Cell Biology 14, 527530.

Keywords

Extracellular matrix alterations in experimental murine Leishmania (L.) amazonensis infection

  • A. L. ABREU-SILVA (a1) (a2), K. S. CALABRESE (a2), R. A. MORTARA (a3), R. C. TEDESCO (a4) (a5), F. O. CARDOSO (a2), L. O. P. CARVALHO (a2) and S. C. GONÇALVES DA COSTA (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed