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Effect of parasitism on plasma sex-specific proteins in Cyphocarax gilbert (Teleost, Curimatidae)

  • L. GOMES DA SILVA (a1), J. S. AZEVEDO (a1), M. A. SILVA-NETO (a2), N. R. WILLE LIMA (a3) and M. DANSA-PETRETSKI (a1)...


Cyphocarax gilbert (Szidat, L., 1948) is a fish commonly found in coastal drainage of eastern Brazil. This fish is sometimes caught with signs of infection by the crustacean Riggia paranensis, a haematophagous parasite. A remarkable feature of infected fish is that they lack gonads. In this paper we have analysed the frequency of parasitism, the gonadal development of non-infected fish and the profile of plasma proteins in both infected and non-infected specimens. Two reproductive periods/year were observed, beginning in February and August. On average, 40% of fish were infected, in the Itabapoana River (Brazil). Sex-specific proteins were identified by electrophoresis. SDS-PAGE analysis demonstrated that a 143 kDa female-specific glycolipoprotein (FSP) is a calcium-binding phosphoprotein. FSP was isolated through ultracentrifugation and SDS-PAGE analysis showed that the native protein is composed of three polypeptides of 143, 100 and 70 kDa. Both FSP and a 33 kDa male-specific protein (MSP) are absent from infected fish plasma. FSP levels in female plasma changes with the developmental stage of gonads. Altogether these data suggest that the FSP corresponds to fish vitellogenin. Furthermore, the absence of the above-mentioned proteins in infected fish suggests that R. paranensis might interfere with the regular hormonal process of fish vitellogenesis.


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LQFPP, CBB, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, P5 sala 228, CEP: 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil. Fax: +55 22 27261437. E-mail:


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ADLARD, R. D. & LESTER, J. G. ( 1994). Dynamic of the interaction between the parasitic isopod, Anilocra pomacentri, and the coral reef fish, Chromis nitida. Parasitology 94, 311324.
ADLARD, R. D. & LESTER, R. J. G. ( 1995). The life cycle and biology of Anilocra pomacentri (Isopoda, Cymothoidae) and ectoparasitic isopod of coral-reef fish, Chromis nitida (Perciformes, Pomacentridae). Australian Journal of Zoology 43, 271281.
ANDRADE, D. R. & GODINHO, H. ( 1983). Annual male reproductive cycle of the Brazilian teleost fish Leporinus silvestrii (Boulenger, 1902). Archives de Biologie (Bruxelles) 94, 114.
AZEVEDO, J. S., THOMÉ, M. P. M., GOMES DA SILVA, L., NOVELLI, R., DANSA-PETRETSKI, M. & WILLE LIMA, N. R. ( 2002). Parasitismo de Riggia paranensis (Crustacea, Cymothoidea) em populações de Cyphocharax gilbert (Teleostei, Curimatidae) do norte do estado do Rio de Janeiro. Boletim do Instituto de Pesca, São Paulo 28, 6169.
BRAGONI, G., ROMESTAND, B. & TRILLES, J. P. ( 1983). Cymothoadian parasitosis of the sea-dace (Dicentrarchus labrax Linnaeus, 1758) during breeding. II. Parasitic ecophysiology in the Diana pod. Annales de Parasitologie Humaine et Comparée 58, 593609.
BUJO, H., HERMANN, M., LINDSTEDT, K. A., NIMPF, J. & SCHNEIDER, W. J. ( 1997). Low density lipoprotein receptor gene family members mediate yolk deposition. Journal of Nutrition 127 (Suppl.), 801S804S.
CALLARD, I. P., RILEY, D. & PERES, L. ( 1990). Vertebrate vitellogenesis: molecular model for multihormonal control of gene regulation. Progress in Clinical and Biological Research 342, 343348.
CUTTING, J. A. & ROTH, T. F. ( 1973). Staining of phosphoproteins on acrylamide gel electropherograms. Analytical Biochemistry 54, 386394.
DAVIS, B. J. ( 1964). Disc electrophoresis – II Methods and application to human serum proteins. Annals of the New York Academy of Sciences 121, 404427.
HUIZINGA, H. W. ( 1972). Pathology of Artystone trysibia Schioedte (Isopoda: Cymothoidae), an endoparasitic isopod of South American fresh water fish. Journal of Wildlife Diseases 8, 225233.
KOYA, Y., MATSUBARA, T., IKEUCHI, T., ADACHI, S. & YAMAUCHI, K. ( 1997). Annual changes in serum vitellogenin concentrations in viviparous eelpout, Zoarces elongatus. Advances in Comparative Physiology and Biochemistry 118, 12171223.
LAFFERTY, K. D. & KURIS, A. M. ( 1999). How environment stress affects the impact of parasites. Limnology and Oceanography 44, 925931.
LAEMMLI, U. K. ( 1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680685.
LOWRY, O. H., FARR, A. L., ROSEBROUGH, N. J. & RANDALL, R. J. ( 1951). Protein measurement with Folin phenol reagent. The Journal of Biological Chemistry 193, 265275.
MACKENZIE, D., THOMAS, P. & FARRAR, S. M. ( 1989). Seasonal changes in thyroid and reproductive steroid hormones in female channel catfish (Itaclurus punctatus) in pond culture. Aquaculture 78, 6380.
MARKS, R. E., JUANES, F., HARE, J. A. & CONOVER, D. O. ( 1996). Occurrence and effect of the parasitic isopod, Lironeca ovalis (Isopoda: Cymothoidae), on young-of-the-year bluefish, Pomatomus saltatrix (Pisces: Pomacentridae). Canadian Journal of Fisheries and Aquatic Sciences 53, 20522057.
MARUYAMA, K., MIKAWA, T. & EBASHI, S. ( 1984). Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. Journal of Biochemistry (Tokyo) 95, 511519.
MENEZES, M. S. ( 1994). Estrutura da população e reprodução de Cyphocarax gilbert (Quoy and Gaimard, 1824) (Osteichthyes, Curimatidae) no trecho inferior do rio Paraíba do Sul (RJ, MG) e principais afluentes. Dissertação de Mestrado (MSc). Universidade Federal do Paraná, Curitiba, PR.
NAGAHAMA, Y. ( 1983). The functional morphology of teleost gonads. In Fish Physiology, vol. IXA, (ed. Hoar, W. S. & Randall, D. J.), pp. 223264. Academic Press, London.
RADUJKOVIC, B. M. ( 1991). Parasitic crustaceans of Adriatic fishes. Wiadomosci Parazytologiczne 37, 149150.
RAIBAUT, A. & TRILLES, J. P. ( 1993). The sexuality of parasitic crustaceans. Advances in Parasitology 32, 367444.
RETAMAL, C. A., THIEBAUT, P. & ALVES, E. W. ( 1999). Protein purification from polyacrylamide gels by sonication extraction. Analytical Biochemistry 268, 1520.
ROMESTAND, B., JANICOT, M. & TRILLES, J. P. ( 1977). Modifications tissulaires et réactions de défences chez quelques Téléostéens parasites par les Cymothoidea (Crustacés Isopodes hématophages). Annales de Parasitologie Humaine et Comparée 52, 171180.
SCHAEFER, S. A. ( 1993). A remarkable occurrence of isopod parasitism on an armoured catfish, Microlepidogaster maculipinnis. Journal of Fish Biology 42, 307310.
SCHNEIDER, W. J. ( 1996). Vitellogenin receptors: oocyte- specific members of the low-density lipoprotein receptor supergene family. International Review of Cytology 166, 103137.
SILVA-NETO, M. A., ATELLA, G. C., FIALHO, E., PAES, M. C., ZINGALI, R. B., PETRETSKI, J. H., ALVES, E. W. & MASUDA, H. ( 1996). Isolation of a calcium-binding phosphoprotein from the oocytes and hemolymph of the blood-sucking insect Rhodnius prolixus. The Journal of Biological Chemistry 271, 3022730232.
SZIDAT, L. ( 1948). Riggia paranenis n.g., n. sp. un isópodo parásito de la cavidad del cuerpo de “Curimata platana” Günther del Rio Paraná. Revista del Instituto de Investigación de las Ciencias Naturales Ciencias Zoológicas, Buenos Aires 1, 4546.
TATA, J. R. & SMITH, D. F. ( 1978). Vitellogenesis. A versatile model for hormonal regulation of gene expression. Recent Progress in Hormone Research 35, 4790.
THATCHER, V. E. ( 1991). Amazon fish parasites. Amazoniana 11, 263572.
THOMÉ, M. P. M. ( 1997). Influência do parasita Riggia paranensis (Crustacea: Cymothoidae) no sairú Cyphocarax gilbert (Pisces, Curimatidae) no complexo lagoa de Cima-lagoa Feia. Monografia. Universidade Estadual do Norte Fluminense.
TOFT, C. ( 1991). An ecological perspective: the population and community consequences of parasitism. In Parasite-Host Associations (ed. Toft, C. A., Aeschlimann, A. & Bolis, L.), pp. 319343. Oxford Science Publication, New York, USA.
TRILLES, J. P. ( 1991). Catalogue mondial des Cymothoidae. Studia Marina, Kotor 21/22, 5288.
TYLER, C. R. & LUBBERINK, K. ( 1996). Identification of four ovarian receptor proteins that bind vitellogenin but not other homologous plasma lipoproteins in the rainbow trout, Oncorhynchus mykiss. Journal of Comparative Physiology B 166, 1120.
UTARABHAND, P. & BUNLIPATANON, P. ( 1996). Plasma vitellogenin of grouper (Epinephelus malabaricus): isolation and properties. Journal of Comparative Biochemistry and Physiology C 115, 101110.
WALLACE, R. A. & SELMAN, K. ( 1985). Major protein changes during vitellogenesis and maturation of Fundulus oocytes. Developmental Biology 110, 492498.


Effect of parasitism on plasma sex-specific proteins in Cyphocarax gilbert (Teleost, Curimatidae)

  • L. GOMES DA SILVA (a1), J. S. AZEVEDO (a1), M. A. SILVA-NETO (a2), N. R. WILLE LIMA (a3) and M. DANSA-PETRETSKI (a1)...


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