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Echinococcus granulosus cyst fluid suppresses inflammatory responses by inhibiting TRAF6 signalling in macrophages

Published online by Cambridge University Press:  29 March 2021

Ke Lin ,
Di Zhou ,
Min Li ,
Jin Meng ,
Feiming He ,
Xiaofeng Yang ,
Dan Dong Open the ORCID record for Dan Dong [Opens in a new window] ,
Xian Wang ,
Xiangwei Wu  and
Xueling Chen Open the ORCID record for Xueling Chen [Opens in a new window]
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Ke Lin
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Di Zhou
Affiliation:
Medical Laboratory, the First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Min Li
Affiliation:
Nursing School of Shihezi University, Shihezi, Xinjiang, China
Jin Meng
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Feiming He
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Xiaofeng Yang
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Dan Dong
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Xian Wang
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Xiangwei Wu
Affiliation:
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Xueling Chen*
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
Jun Hou*
Affiliation:
Department of Immunology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China
*
Authors for correspondence: Xueling Chen, E-mail: chenxueling@shzu.edu.cn; Jun Hou, E-mail: houjun229@163.com
Authors for correspondence: Xueling Chen, E-mail: chenxueling@shzu.edu.cn; Jun Hou, E-mail: houjun229@163.com
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Abstract

Echinococcus granulosus sensu lato has complex defence mechanisms that protect it from the anti-parasitic immune response for long periods. Echinococcus granulosus cyst fluid (EgCF) is involved in the immune escape. Nevertheless, whether and how EgCF modulates the inflammatory response in macrophages remains poorly understood. Here, real-time polymerase chain reaction and enzyme-linked immunosorbent assay revealed that EgCF could markedly attenuate the lipopolysaccharide (LPS)-induced production of pro-inflammatory factors including tumour necrosis factor-α, interleukin (IL)-12 and IL-6 but increase the expression of IL-10 at mRNA and protein levels in mouse peritoneal macrophages and RAW 264.7 cells. Mechanically, western blotting and immunofluorescence assay showed that EgCF abolished the activation of nuclear factor (NF)-κB p65, p38 mitogen-activated protein kinase (MAPK) and ERK1/2 signalling pathways by LPS stimulation in mouse macrophages. EgCF's anti-inflammatory role was at least partly contributed by promoting proteasomal degradation of the critical adaptor TRAF6. Moreover, the EgCF-promoted anti-inflammatory response and TRAF6 proteasomal degradation were conserved in human THP-1 macrophages. These findings collectively reveal a novel mechanism by which EgCF suppresses inflammatory responses by inhibiting TRAF6 and the downstream activation of NF-κB and MAPK signalling in both human and mouse macrophages, providing new insights into the molecular mechanisms underlying the E. granulosus-induced immune evasion.

Keywords

Cystic echinococcosisEgCFinflammationmacrophagesTRAF6
Type
Research Article
Information
Parasitology , Volume 148 , Issue 7 , June 2021 , pp. 887 - 894
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this study.

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