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Diversity of Karyolysus and Schellackia from the Iberian lizard Lacerta schreiberi with sequence data from engorged ticks

Published online by Cambridge University Press:  02 September 2019

Kristína Zechmeisterová ,
Joëlle Goüy de Bellocq  and
Pavel Široký Open the ORCID record for Pavel Široký [Opens in a new window]
Kristína Zechmeisterová
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno 612 42, Czech Republic
Joëlle Goüy de Bellocq
Affiliation:
Institute of Vertebrate Biology, Research Facility Studenec, The Czech Academy of Sciences, Květná 170/8, Brno 603 65, Czech Republic
Pavel Široký*
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, Brno 612 42, Czech Republic CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, 612 42 Brno, Czech Republic
*
Author for correspondence: Pavel Široký, E-mail: sirokyp@vfu.cz
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Abstract

Apicomplexan haemoparasites of the genera Schellackia Reichenow, 1919, and Karyolysus Labbé, 1894, seem to be common in lizards and widespread across the world. For decades, their identification has been based on morphological descriptions and life cycle patterns, with molecular characterizations, applied only recently. We used molecular characterization to confirm the identification of haemoparasites detected by microscopy in blood smears of Lacerta schreiberi Bedriaga, 1878, a lizard of the Iberian Peninsula. Since blood samples other than blood smears were not available from the studied lizards, 264 engorged ticks Ixodes ricinus (Linneaus, 1758) collected from them were used as an alternative non-invasive source of haemoparasite DNA for molecular genetic analyses. Of the 48 blood smears microscopically examined, 31 were positive for blood parasites (64.6% prevalence). We identified trophozoites and gamonts similar to Karyolysus lacazei (Labbé, 1894) (24/48; 50%) and Schellackia-like sporozoites (20/48; 41.7%). Mixed infections with both species occurred in 13 blood smears (27.1%). Sequence data were obtained for both parasites from engorged ticks. Phylogenetic analyses placed our unique haemogregarine sequence within the Karyolysus clade, nevertheless, within substantial polytomy. Thus, according to its morphology and effect on the host cell, we refer to this haemogregarine as Karyolysus cf. lacazei. Besides the Schellackia sequences being identical to a previously identified haplotype, we also obtained sequences of three new closely related haplotypes.

Keywords

HaemoparasitesIberian PeninsulaIxodes ricinusKaryolysusLacerta schreiberiSchellackia
Type
Research Article
Information
Parasitology , Volume 146 , Issue 13 , November 2019 , pp. 1690 - 1698
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Copyright
Copyright © Cambridge University Press 2019 

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