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Dissociation of early and late protective immunity to the nematode Nippostrongylus brasiliensis in Brown Norway and Fischer-344 rats

Published online by Cambridge University Press:  06 April 2009

R. Uchikawa ,
M. Yamada ,
S. Matsuda ,
T. Tegoshi ,
M. Nishida ,
I. Kamata ,
A. Kuroda  and
N. Arizono
R. Uchikawa
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
M. Yamada
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
S. Matsuda
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
T. Tegoshi
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
M. Nishida
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
I. Kamata
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
A. Kuroda
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
N. Arizono
Affiliation:
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602, Japan
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Summary

Worm expulsion of, and IgE and interferon (IFN)-γ responses to, Nippostrongylus brasiliensis were studied in 2 rat strains, Brown Norway (BN) and Fischer (F)-344. BN rats expelled the majority of worms by day 14 post-infection (p.i.) with approximately 6% of worms surviving for at least 3 weeks. In F-344 rats, worm expulsion was delayed by 2 days relative to that in BN, while the numbers of residual worms were significantly fewer than in BN, suggesting that different immune mechanisms are involved in early and late phases of immunity. Total serum IgE, as well as in vitro IgE production by mesenteric lymph node (MLN) cells, was increased 2 weeks p.i., the levels being markedly higher in BN than in F-344 rats. Serum rat mast cell protease II was also increased more significantly in BN than in F-344 rats. In contrast, production of IgG2a and IFN-γ by MLN and spleen cells was found to be higher in F-344 than in BN rats. These results indicate that the early worm expulsion is correlated with the host IgE and mast cell responsiveness, whereas the persistence of infection in the late period may be controlled by different immune mechanisms.

Keywords

Nippostrongylus brasiliensisprotective immunityIgErat mast cell protease IIinterferon-γinbred rat strain
Type
Research Article
Information
Parasitology , Volume 112 , Issue 3 , March 1996 , pp. 339 - 345
Copyright
Copyright © Cambridge University Press 1996

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