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Dipstick dot ELISA for the detection of Taenia coproantigens in humans

Published online by Cambridge University Press:  06 April 2009

J. C. Allan ,
F. Mencos ,
J. Garcia-Noval ,
E. Sarti ,
A. Flisser ,
Y. Wang ,
D. Liu  and
P. S. Craig
J. C. Allan
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, UK
F. Mencos
Affiliation:
Centro de Investigaciones de Ciencias de la Salud, Facultad de Medicina, Universidad de San Carlos de Guatemala, Ciudad de Guatemala, Guatemala C.A.
J. Garcia-Noval
Affiliation:
Centro de Investigaciones de Ciencias de la Salud, Facultad de Medicina, Universidad de San Carlos de Guatemala, Ciudad de Guatemala, Guatemala C.A.
E. Sarti
Affiliation:
Direccion General de Epidemiologia, Secretaria de Salud, Francisco de P. Miranda 177, Col. Lomas de Plateros. C.P. 01480, Mexico D.F., Mexico
A. Flisser
Affiliation:
Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 04510 Mexico D.F., Mexico
Y. Wang
Affiliation:
Department of Public Health, Min County, Gansu, Peoples Republic of China
D. Liu
Affiliation:
Department of Parasitology, Lanzhou Medical College, Lanzhou, Gansu, Peoples Republic of China
P. S. Craig
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, UK
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Summary

A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were adsorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and dried the antibody-coated dipsticks were stable for several weeks at room temperature. Both micro and dot ELISA formats were genus specific although the dot ELISA was less sensitive than the micro ELISA based on the same antiserum. During field studies, in which the majority of samples were tested in rural villages soon after collection, 3728 samples were tested. All samples were also examined by microscopy using formol ether concentration and individuals questioned to determine whether they were aware of being infected. After the initial diagnostic work individuals were treated with taeniacidal drugs for worm recovery. Use of the coproantigen test significantly increased the number of cases diagnosed. Of the 41 cases diagnosed by the three diagnostic techniques combined 31 were detected by the dipstick assay making it the most sensitive technique employed. The specificity of the dipstick assay was 99·9% with a positive predictive value of 88·%. The combined diagnostic approach did not, however, diagnose all cases. The coproantigen test was fast and easy to use. Further improvements may make the dipstick test suitable for wide-scale use in field studies and diagnostic laboratories.

Keywords

immunodiagnosiscoproantigenTaeniadot ELISA
Type
Research Article
Information
Parasitology , Volume 107 , Issue 1 , July 1993 , pp. 79 - 85
Copyright
Copyright © Cambridge University Press 1993

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