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Differential polyadenylation of ribosomal RNA during post-transcriptional processing in Leishmania

Published online by Cambridge University Press:  10 May 2005

S. DECUYPERE ,
J. VANDESOMPELE ,
V. YARDLEY ,
S. DE DONCKER ,
T. LAURENT ,
S. RIJAL ,
A. LLANOS-CUENTAS ,
F. CHAPPUIS ,
J. AREVALO  and
J.-C. DUJARDIN
S. DECUYPERE
Affiliation:
Department of Parasitology, Unit of Molecular Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp B-2000, Belgium Department of Biomedical Sciences, University of Antwerp, Antwerp B-2080, Belgium
J. VANDESOMPELE
Affiliation:
Centre for Medical Genetics, Ghent University Hospital 1K5, Ghent B-9000, Belgium
V. YARDLEY
Affiliation:
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E7HT, UK
S. DE DONCKER
Affiliation:
Department of Parasitology, Unit of Molecular Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp B-2000, Belgium
T. LAURENT
Affiliation:
Department of Parasitology, Unit of Molecular Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp B-2000, Belgium
S. RIJAL
Affiliation:
B.P. Koirala Institute of Health Sciences, Dharan, Nepal
A. LLANOS-CUENTAS
Affiliation:
Departamento de Bioquimica, Biologia Molecular y Farmacologia, Facultad de Ciencias y Filosofia and Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima 100, Peru
F. CHAPPUIS
Affiliation:
Department of Community Medicine, Travel and Migration Medicine Unit, Hopitaux Universitaires de Geneve, Geneva, Switzerland
J. AREVALO
Affiliation:
Departamento de Bioquimica, Biologia Molecular y Farmacologia, Facultad de Ciencias y Filosofia and Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima 100, Peru
J.-C. DUJARDIN
Affiliation:
Department of Parasitology, Unit of Molecular Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp B-2000, Belgium
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Abstract

The protozoan parasite Leishmania belongs to the most ancient eukaryotic lineages and this is reflected in several distinctive biological features, such as eukaryotic polycistronic transcription and RNA trans-splicing. The disclosure of this organism's unusual characteristics leads to a better understanding of the origin and nature of fundamental biological processes in eukaryotes. Here we report another unusual phenomenon as we demonstrate that precursor ribosomal RNA can be extensively polyadenylated during post-transcriptional processing[dagger]. Furthermore, we demonstrate that the degree of precursor rRNA polyadenylation is variable in different strains and in the different life-stages of a strain.

Keywords

LeishmaniarRNA polyadenylation
Type
Research Article
Information
Parasitology , Volume 131 , Issue 3 , September 2005 , pp. 321 - 329
Copyright
© 2005 Cambridge University Press

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