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Development of larval Echinococcus granulosus confronted with chicken heart tissue in vitro

Published online by Cambridge University Press:  06 April 2009

D. Janssen ,
P. H. De Rycke  and
L. De Ridder
D. Janssen
Affiliation:
Laboratory of Zoophysiology, State University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
P. H. De Rycke
Affiliation:
Laboratory of Zoophysiology, State University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
L. De Ridder
Affiliation:
Laboratory of Histology, State University of Gent, L. Pasteurlaan 2, B-9000 Gent, Belgium
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Summary

Vesiculated protoscoleces (VP) were produced by culturing freshly collected protoscoleces from Echinococcus granulosus horse liver hydatids in RPMI 1640 monophasic medium at 37°C for 18 days. Half of the VP were used as such, the other half used after killing them by freeze—thawing. Nine-day-old chicken heart fragments (CHF) were cultured in MEM at 37°C for 72 h. Subsequently, CHF were put together with live and dead VP, respectively, for up to 53 days, on a semisolid medium consisting of agar, Ringer's and MEM. Time-dependent histological observations revealed that dead VP were surrounded by CHF cells. Dead VP tissue was eventually internalized and disintegrated in about 1 week. Live VP penetrated into the CHF tissue and further developed into small hydatid cysts, located within the boundaries of the experimental ‘host’ tissue. The amorphous-looking contact region PAP-stained positively only with anti-E. granulosus serum and not with anti-CHF serum; it was considered identical to the normal laminated layer. The invasion of VP in CHF tissue proved to be different from a tumour or a bacterial invasion: it was concluded that the confrontation of VP and CHF had resulted in an ‘in vitro cohabitation’ rather than in an ‘in vitro infecion’.

Keywords

Echinococcus granulosuslarval developmentin vitrochicken heart
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 453 - 458
Copyright
Copyright © Cambridge University Press 1990

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