Skip to main content Accessibility help

Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric and ribosomal DNA probes and anti-schizont monoclonal antibodies

  • R. P. Bishop (a1), B. K. Sohanpal (a1), B. A. Allsopp (a2), P. R. Spooner (a1), T. T. Dolan (a1) and S. P. Morzaria (a1)...


A total of 21 Theileria parva stocks from 6 countries were characterized using T. parva repetitive and ribosomal DNA probes, a Plasmodium berghei telomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots of EcoRI-digested T. parva DNA revealed 20 different restriction fragment patterns among DNA samples isolated from infections initiated using 16 parasite stocks. The panel of anti-schizont MAbs defined 8 different profiles among schizont-infected lymphoblastoid cell-cultures infected with the same 16 T. parva stocks. Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A cloned T. parva small subunit ribosomal RNA (SSUrRNA) gene probe separated 17 T. parva stocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized to EcoRI-digested T. parva DNA. When hybridized to PvuII-digested DNA from 8 T. parva stocks, the ribosomal probe identified 4 groups with similar restriction fragment patterns. A synthetic oligonucleotide derived from a P. berghei telomeric sequence hybridized to 7 or 8 size-polymorphic restriction fragments in the EcoRI-digested DNA of most T. parva stocks. The telomeric and ribosomal probes defined the same 4 groups among 8 T. parva stocks as assessed by similarities in restriction fragment patterns. Based on the comparison of repetitive DNA sequences from the T. parva Uganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of the T. parva Uganda stock from that of 4 other T. parva stocks on a positive/negative basis.



Hide All
Allsopp, B. A. & Allsopp, M. T. E. P. (1988). Theileria parva: genomic DNA studies reveal intra-specific sequence diversity. Molecular and Biochemical Parasitology 28, 7784.
Allsopp, B. A., Baylis, H. A., Allsopp, M. T. E. P., Cavalier-Smith, T., Bishop, R. P., Carrington, M. D., Sohanpal, B. & Spooner, P. R. (1993). Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences. Parasitology 107 (in the Press.)
Allsopp, B., Carrington, M., Baylis, H., Sohal, S., Dolan, T. & Iams, K. (1989). Improved characterisation of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes. Molecular and Biochemical Parasitology 35, 137–48.
Allsopp, B. A., Gibson, W. C., Stagg, D. A. (1985). Characterisation of some East African Theileria species isolates by isoenzyme analysis, with particular reference to T. parva. International Journal for Parasitology 15, 271–6.
Baylis, H. A., Sohal, S. K., Carrington, M., Bishop, R. P. & Allsopp, B. A. (1991). An unusual repetitive gene family in Theileria parva which is stage specifically transcribed. Molecular and Biochemical Parasitology 49, 133–42.
Biggs, B. A., Kemp, D. J. & Brown, D. V. (1989). Subtelomeric deletions in field isolates of Plasmodium faliparum and their relationship to loss of cytoadherence in vitro. Proceedings of the National Academy of Sciences, USA 86, 2428–32.
Brocklesby, D. W., Barnett, S. F. & Scott, G. R. (1961). Morbidity and mortality rates in East coast fever (Theileria parva infection) and their application to drug screening procedures. British Veterinary Journal 17, 529–31.
Brown, C. G. D. (1979). Propagation of Theileria. In Practical Tissue Culture Applications (ed. Maramorosch, K. & Hirum, H.), pp. 223245. New York: Academic Press.
Brown, W. C. & Grab, D. J. (1985). Biological and biochemical characterisation of bovine interleukin 2. Studies with cloned bovine T cells. Journal of Immunology 133, 3184–9.
Conrad, P. A., Baldwin, C. L., Brown, W. C., Sohanpal, B. A., Dolan, T. T., Goddeeris, B. M., Demartini, J. C. & Ole-Moiyoi, O. K. (1989 b). Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype. Parasitology 99, 205–31.
Conrad, P. A., Iams, K., Brown, W. C., Sohanpal, B. & Ole-Moi Yoi, O. (1987 b). DNA probes detect genomic diversity in Theileria parva stocks. Molecular and Biochemical Parasitology 25, 213–26.
Conrad, P. A., Ole-Moiyoi, O. K., Baldwin, C. L., Dolan, T. T., O'callaghan, C. J., Njamunggeh, R. E. G., Grootenhuis, J. G., Stagg, D. A., Leitch, B. L. & Young, A. S. (1989 a). Characterisation of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology 98, 179–88.
Conrad, P. A., Stagg, D. A., Grootenhuis, J. G., Irvin, A. D., Newson, R. M., Njammungeh, R. E. G., Rossiter, P. B. & Young, A. S. (1987 a). Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies. Parasitology 94, 413–23.
Corcoran, L. M., Thompson, J. K., Walliker, D. & Kemp, D. J. (1988). Homologous recombination within subtelomeric repeat sequences generates chromosome size polymorphisms in Plasmodium falciparum. Cell 53, 807–13.
Dalrymple, B. P. (1990). Cloning and characterisation of the rRNA genes and flanking regions from Babesia bovis: use of the genes as strain discriminating probes. Molecular and Biochemical Parasitology 43, 117–24.
Dietrich, P., Pilar Dussan, M., Floeter-Winter, L. M., Trebilcock, M. H., Camargo, E. P. & Bento Soares, M. (1990). Restriction fragment length polymorphisms in the ribosomal gene spacers of Trypanosoma cruzi and Trypanosoma conorhini. Molecular and Biochemical Parasitology 42, 1320.
Dolan, T. T., Radley, D. E., Brown, C. G. D., Cunningham, M. P., Morzaria, S. P. & Young, A. S. (1980). East Coast fever: further studies on the protection of cattle immunised with a combination of theilerial strains. Veterinary Parasitology 6, 325–32.
Higgins, D. G. & Sharp, P. M. (1988). CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73, 237–44.
Irvin, A. D. (1987). Characterisation of species and strains of Theileria. Advances in Parasitology 26, 145–79.
Irvin, A. D., Dobbelaere, D. A. E., Mwamachi, D. M., Minami, M., Spooner, P. R. & Ocama, J. G. R. (1983). Immunisation against East Coast fever: Correlation between monoclonal antibody profiles of Theileria parva stocks and cross-immunity in vivo. Research in Veterinary Science 35, 341–6.
Lawrence, J. A. & Mackenzie, P. K. I. (1980). Isolation of a non-pathogenic Theileria of cattle transmitted by Rhipicephalus appendiculatus. Zimbabwe Veterinary Journal 11, 2735.
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: a Laboratory Manual. Cold Spring Harbour, New York: Cold Spring Harbour Laboratory.
Mehlhorn, H. & Schein, E. (1984). The piroplasms: life cycle and sexual stages. Advances in Parasitology 23, 37103.
Melrose, T. R. & Brown, C. G. D. (1979). Isoenzyme variation in piroplasms isolated from bovine blood infected with Theileria annulata and T. parva. Research in Veterinary Science 27, 379–81.
Minami, T., Spooner, P. R., Irvin, A. D., Ocama, J. G. R., Dobbelaere, D. A. E. & Fujinaga, T. (1983). Characterisation of stocks of Theileria parva by monoclonal antibody profiles. Research in Veterinary Science 35, 334–40.
Morzaria, S. P., Spooner, S. P., Bishop, R. P., Musoke, A. J. & Young, J. R. (1990). SfiI and NotI polymorphisms in Theileria stocks detected by pulsed field gel electrophoresis. Molecular and Biochemical Parasitology 40, 203–12.
Morzaria, S. P. & Young, J. R. (1992). Restriction mapping of the genome of the protozoan parasite Theileria parva. Proceedings of the National Academy of Sciences, USA 89, 5241–5.
Mutugi, J. J., Young, A. S., Maritim, A. C., Ndungu, S. K., Mining, S. K., Linyonyi, A., Ngumi, P. N., Leitch, B. L., Morzaria, S. P. & Dolan, T. T. (1989). Immunization of cattle in Coast Province Kenya: laboratory evaluation of a Theileria parva parva stabilate for use in infection and treatment immunization in the field. Research in Veterinary Science 27, 170–7.
Ponzi, M., Pace, T., Dore, E. & Frontali, C. (1985). Identification of a telomeric DNA sequence in Plasmodium berghei. The EMBO Journal 4, 2991–5.
Radley, D. E., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M., Purnell, R. E. & Young, A. S. (1975). East coast fever, 1. Chemoprophylactic immunization of cattle against Theileria parva (Muguga) and five theilerial strains. Veterinary Parasitology 1, 3541.
Shapiro, S. Z., Fujisaki, K., Morzaria, S. P., Webster, P., Fujinaga, T., Spooner, P. R. & Irvin, A. D. (1987). A life-cycle stage-specific antigen of Theileria parva recognized by anti-schizont monoclonal antibodies. Parasitology 94, 2937.
Stagg, D. A., Young, A. S., Leitch, B. L., Grootenhuis, J. G. & Dolan, T. T. (1983). Infection of mammalian cells with Theileria species. Parasitology 86, 243–54.
Sugimoto, C., Conrad, P. A., Mutharia, L., Dolan, T. T., Brown, W. C., Goddeeris, B. M. & Pearson, T. W. (1989). Phenotypic characterisation of Theileria parva schizonts by two-dimensional gel electrophoresis. Parasitology Research 76, 17.
Toye, P. G., Goddeeris, B. M., Iams, K., Musoke, A. J. & Morrison, W. I. (1991). Characterisation of a polymorphic immunodominant molecule in sporozoites and schizonts of Theileria parva. Parasite Immunology 13, 4962.
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. & Young, A. S.), pp. 437. The Hague: Martinus Nijhoff.
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M. & Irvin, A. D. (1973). Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle. International Journal for Parasitology 3, 723–8.


Related content

Powered by UNSILO

Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric and ribosomal DNA probes and anti-schizont monoclonal antibodies

  • R. P. Bishop (a1), B. K. Sohanpal (a1), B. A. Allsopp (a2), P. R. Spooner (a1), T. T. Dolan (a1) and S. P. Morzaria (a1)...


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.