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Characterization of D-glucose transport in Trypanosoma rangeli

  • L. C. MILETTI (a1), L. B. KOERICH (a2), L. K. PACHECO (a2), M. STEINDEL (a2) and B. U. STAMBUK (a1)...


Like in other trypanosomatids D-glucose is a crucial source of energy to Trypanosoma rangeli, a non-pathogenic parasite that in Central and South America infects triatomine vectors and different mammalian species, including humans. In several trypanosome species, D-glucose transporters were already described and cloned. In this study, we characterized the D-glucose transport activity present in 2 life-stage forms of T. rangeli (epimastigotes and trypomastigotes) using D-[U-14C]glucose as substrate. Our results indicate that T. rangeli transports D-glucose with high affinity in both epimastigote (Km 30 μM) and trypomastigotes (Km 80 μM) life-forms. Both transport activities were inhibited by Cytochalasin B and Phloretin, indicating that probably D-glucose uptake in T. rangeli is mediated by facilitated diffusion of the sugar. Significant differences were observed between epimastigotes and trypomastigotes in relation to their affinity for D-glucose analogues, and the predicted amino acid sequence of a putative D-glucose transporter from T. rangeli (TrHT1) showed a larger identity with the T. cruziD-glucose transporter encoded by the TcrHT1 gene than with other transporters already characterized in trypanosomatids.


Corresponding author

Departamento de Morfofisiologia, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Bairro Conta Dinheiro, SC – CEP 88520-000, Brasil. Tel: +55 49 3221 2200. Fax: +55 49 3221 2222. E-mail:


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Characterization of D-glucose transport in Trypanosoma rangeli

  • L. C. MILETTI (a1), L. B. KOERICH (a2), L. K. PACHECO (a2), M. STEINDEL (a2) and B. U. STAMBUK (a1)...


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