Skip to main content Accessibility help
×
Home

Characterization of D-glucose transport in Trypanosoma rangeli

  • L. C. MILETTI (a1), L. B. KOERICH (a2), L. K. PACHECO (a2), M. STEINDEL (a2) and B. U. STAMBUK (a1)...

Abstract

Like in other trypanosomatids D-glucose is a crucial source of energy to Trypanosoma rangeli, a non-pathogenic parasite that in Central and South America infects triatomine vectors and different mammalian species, including humans. In several trypanosome species, D-glucose transporters were already described and cloned. In this study, we characterized the D-glucose transport activity present in 2 life-stage forms of T. rangeli (epimastigotes and trypomastigotes) using D-[U-14C]glucose as substrate. Our results indicate that T. rangeli transports D-glucose with high affinity in both epimastigote (Km 30 μM) and trypomastigotes (Km 80 μM) life-forms. Both transport activities were inhibited by Cytochalasin B and Phloretin, indicating that probably D-glucose uptake in T. rangeli is mediated by facilitated diffusion of the sugar. Significant differences were observed between epimastigotes and trypomastigotes in relation to their affinity for D-glucose analogues, and the predicted amino acid sequence of a putative D-glucose transporter from T. rangeli (TrHT1) showed a larger identity with the T. cruziD-glucose transporter encoded by the TcrHT1 gene than with other transporters already characterized in trypanosomatids.

Copyright

Corresponding author

Departamento de Morfofisiologia, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Bairro Conta Dinheiro, SC – CEP 88520-000, Brasil. Tel: +55 49 3221 2200. Fax: +55 49 3221 2222. E-mail: lcmilett@yahoo.com.br

References

Hide All

REFERENCES

Amorim, M. I., Momen, H. and Traub-Cseko, Y. M. ( 1993). Trypanosoma rangeli: sequence analysis of beta-tubulin gene suggests closer relationship to Trypanosoma brucei than to Trypanosoma cruzi. Acta Tropica 53, 99105.
Azema, L., Claustre, S., Alric, I., Blonski, C., Willson, M., Perie, J., Baltz, T., Tetaud, E., Bringaud, F., Cottem, D., Opperdoes, F. R. and Barrett, M. P. ( 2004). Interaction of substituted hexose analogues with the Trypanosoma brucei hexose transporter. Biochemical Pharmacology 67, 459467.
Bakker, B. M., Walsh, M. C., Ter Kuile, B. H., Mensonides, F. I., Michels, P. A., Opperdoes, F. R. and Westerhoff, H. V. ( 1999). Contribution of glucose transport to the control of the glycolytic flux in Trypanosoma brucei. Proceedings of National Academy of Sciences, USA 96, 1009810103.
Barrett, M. P., Tetaud, E., Seyfang, A., Bringaud, F. and Baltz, T. ( 1995). Functional expression and characterization of the Trypanosoma brucei procyclic glucose transporter, THT2. The Biochemical Journal 312, 687691.
Barrett, M. P., Tetaud, E., Seyfang, A., Bringaud, F. and Baltz, T. ( 1998). Trypanosome glucose transport. Molecular and Biochemical Parasitolology 91, 195205.
Bayele, H. K. ( 2001). Triazinyl derivatives that are potent inhibitors of glucose transport in Trypanosoma brucei. Parasitology Research 87, 911914.
Bringaud, F. and Baltz, T. ( 1993). Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei. Molecular and Cellular Biology 13, 11461154.
Bringaud, F. and Baltz, T. ( 1994). African trypanosome glucose transporter genes: Organization and evolution of a multigene family. Molecular Biology and Evolution 11, 220230.
Burchmore, R. J. S. and Hart, D. T. ( 1995). Glucose transport in amastigotes and promastigotes of Leishmania mexicana mexicana. Molecular and Biochemical Parasitology 74, 7786.
Bursell, E. ( 1981). The role of proline in energy metabolism In Energy Metabolism in Insects ( ed. Downer, R. G. H.), pp. 135154. Plenum Press, New York.
Coura, J. R., Fernandes, O., Arboleda, M., Barrett, T. V., Carrara, N., Degrave, W. and Campbell, D. A. ( 1996). Human infection by Trypanosoma rangeli in Brazilian Amazon. Transactions of Royal Society of Tropical Medicine and Hygiene 90, 278279.
Cuba, C. A. C. ( 1998). Revisión de los aspectos biológicos y diagnósticos del Trypanosoma (Herpetosoma) rangeli. Revista da Sociedade Brasileira de Medicina Tropical 31, 207220.
D'Alessandro, A. and Saravia, N. G. ( 1992). Trypanosoma rangeli. In Parasitic Protozoa ( ed. Kreier, J. P. and Baker, J.), pp. 154. Academic Press, Oxford.
De Santa Izabel, A., Vermelho, A. B. and Branquinha, M. H. ( 2004). Proteolytic activities in Trypanosoma rangeli and stercorarian trypanosomes: taxonomic implications. Parasitology Research 94, 342348.
Eisenthal, R., Game, S. and Holman, G. D. ( 1989). Specificity and kinetics of hexose transport in Trypanosoma brucei. Biochimica et Biophysica Acta 985, 8189.
Grisard, E. C., Steindel, M., Guarneri, A. A., Eger-Mangrich, I., Campbell, D. A. and Romanha, A. J. ( 1999). Characterization of Trypanosoma rangeli strains isolated in Central and South America: An overview. Memórias do Instituto Oswaldo Cruz 94, 203209.
Grisard, E. C. ( 2002). Salivaria or Stercoraria? The Trypanosoma rangeli dilemma. Kinetoplastid Biology and Disease 1, 5.
Gruenberg, J., Sharma, P. R. and Deshusses, J. ( 1978). d-Glucose transport in Trypanosoma brucei. European Journal of Biochemistry 89, 461469.
Guhl, F. and Vallejo, G. A. ( 2003). Trypanosoma (Herpetosoma) rangeli Tejera,1920 – An update review. Memórias do Instituto Oswaldo Cruz 98, 435442.
Knodler, L. A., Schofield, P. J. and Edwards, M. R. ( 1992). Glucose transport in Crithidia luciliae. Molecular and Biochemical Parasitology 52, 114.
Koerich, L. B., Emanuelle-Neto, P., Santos, K., Grisard, E. C. and Steindel, M. ( 2002). Differentiation of Trypanosoma rangeli: high production of infective trypomastigotes forms in vitro. Parasitology Research 88, 2125.
Langford, C. K., Burchmore, R. J. S., Hart, D. T., Wagner, W. and Landfear, S. M. ( 1994). Biochemistry and molecular genetics of Leishmania glucose transporters. Parasitology 108 (Suppl.), S73S83.
Munoz-Antonia, T., Richards, F. F. and Ullu, E. ( 1991). Differences in glucose transport between bloodstream and procyclic forms of Trypanosoma brucei rhodesiense. Molecular and Biochemical Parasitology 47, 7382.
Osorio, Y., Travi, B., Palma, G. I. and Saravia, N. G. ( 1995). Infectivity of Trypanosoma rangeli in a promonocitic mammalian cell line. Journal of Parasitology 81, 687693.
Ramirez, L. E., Machado, M. I., Maywald, P. G., Matos, A., Chiari, E. and Silva, E. L. ( 1998). First evidence of Trypanosoma rangeli in the southeast of Brazil, an endemic region for Chagas' disease. Revista da Sociedade Brasileira de Medicina Tropical 31, 99102.
Silber, A. M., Tonelli, R. R., Martinelli, M., Colli, W. and Alves, M. J. M. ( 2002). Active transport of l-proline in Trypanosoma cruzi. Journal of Eukaryotic Microbiology 49, 441446.
Snoeijer, C. Q., Picchi, G. F., Dambros, B. P., Steindel, M., Goldenberg, S., Fragoso, S. P., Lorenzini, D. M. and Grisard, E. C. ( 2004). Trypanosoma rangeli Transcriptome Project: Generation and analysis of expressed sequence tags. Kinetoplastid Biology and Disease 3, 1.
Szablewski, L. ( 2000). Facilitated hexose diffusion in kinetoplastida. Acta Protozoologica 39, 183189.
Ter Kuile, B. H. ( 1997). Adaptation of metabolic enzyme activities of Trypanosoma brucei promastigotes to growth rate and carbon regimen. Journal of Bacteriology 179, 46994705.
Ter Kuile, B. H. and Opperdoes, F. R. ( 1991). Glucose uptake by Trypanosoma brucei. Rate-limiting steps in glycolysis and regulation of the glycolytic flux. Journal of Biological Chemistry 266, 857862.
Tetaud, E., Bringaud, F., Chabas, S., Barrett, M. P. and Baltz, T. ( 1994). Characterization of glucose transport and cloning of a hexose transport gene in Trypanosoma cruzi. Proceedings of the National Academy Sciences, USA 91, 82788282.
Tetaud, E., Barrett, M. P., Bringaud, F. and Baltz, T. ( 1997). Kinetoplastid glucose transporters. The Biochemical Journal 325, 569580.
Urdaneta-Morales, S. and Tejero, F. ( 1986). Trypanosoma (Herpetosoma) rangeli Tejera, 1920. Intracellular amastigote stages of reproduction in white mice. Revista do Instituto de Medicina Tropical de São Paulo 28, 166169.
Vallejo, G. A., Guhl, F., Carranza, J. C., Lozano, L. E., Sánchez, J. L., Jaramilo, J. C., Galtero, D., Castaneda, N., Silva, J. C. and Steindel, M. ( 2002). kDNA markers define two major Trypanosoma rangeli lineages in Latin-America. Acta Tropica 81, 7782.
Vedrenne, C., Bringaud, F., Barret, M. P., Tetaud, E. and Baltz, T. ( 2000). The structure-function relationship of functionally distinct but structurally similar hexose transporters from Trypanosoma congolense. European Journal of Biochemistry 267, 48504860.
Waitumbi, J. N., Tetaud, E. and Baltz, T. ( 1996). Glucose uptake in Trypanosoma vivax and molecular characterization of its transporter gene. European Journal of Biochemistry 237, 234239.

Keywords

Related content

Powered by UNSILO

Characterization of D-glucose transport in Trypanosoma rangeli

  • L. C. MILETTI (a1), L. B. KOERICH (a2), L. K. PACHECO (a2), M. STEINDEL (a2) and B. U. STAMBUK (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.