Skip to main content Accessibility help
×
Home

Changes in nematode antigens recognized by monoclonal antibodies during early infections of soya beans with the cyst nematode Heterodera glycines

  • H. J. Atkinson (a1) and P. D. Harris (a1)

Summary

The invasion and establishment of Heterodera glycines has been studied for 0–120 h after addition to host roots using a synchronized infection system. A high degree of synchrony in development was noted, with juvenile nematodes reaching their feeding sites adjacent to the root endodermis and discharging their subventral pharyngeal glands by 24 h post-invasion (p.i.). The moult to the 3rd-stage juvenile occurred approximately 89 h after entry to the root. Using an enzyme-linked immunosorbent assay with monoclonal antibodies (MAbs) whose tissue specificities had already been defined by immuno-cytochemistry, it was found that MAbs recognizing intestinal lipid droplets and granules showed a major increase in reactivity 18–24 h p.i. before declining by 39 h p.i. In contrast, the reactivity of two MAbs recognizing adult cuticle increased significantly only after 89 h p.i., following the second moult. MAbs to the two subventral pharyngeal gland cells showed a variety of patterns of changing reactivity during the experiment. One increased within 24 h of invasion before declining whereas a second fell without any initial rise. Two further MAbs specific for the subventral glands initially declined but showed a secondary increase in reactivity after 72 h and could be detected in adult females. This pattern was also seen in a MAb specific for the dorsal pharyngeal gland cell. These changes are discussed in the context of events taking place following invasion, with particular reference to the initiation and maintenance of a feeding syncytium by the developing nematode.

Copyright

References

Hide All
Anon. (1987). Bibliography of estimated crop losses in the United States due to plant parasitic nematodes. Annals of Applied Nematology 1, 612.
Atkinson, H. J. (1986). The energetics of plant parasitic nematodes — a review. Nematologica 31, 6271.
Atkinson, H. J., Harris, P. D., Halk, E.L., Novitski, C., Nolan, P., Leighton-Sands, J. & Fox, P. C. (1988). Monoclonal antibodies to the soya bean cyst nematode, Heterodera glycines. Annals of Applied Biology 112, 459–69.
Bird, A. F. (1959). Development of the root knot nematodes Meloidogyne javanica (Treub) and Meloidogyne hapla Chitwood in the tomato. Nematologica 4, 3142.
Bird, A. F. (1967). Changes associated with parasitism in nematodes. 1. Morphology and physiology of preparasitic and parasitic larvae of Meloidogyne javanica. Journal of Parasitology 53, 768–76.
Bird, A. F. (1979). The Structure of Nematodes. New York and London: Academic Press.
Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Analytical Biochemistry 72, 248–54.
Byrd, D. W., Kirkpatrick, T. & Barker, K. R. (1983). An improved technique for clearing and staining plant tissues for detection of nematodes. Journal of Nematology 15, 142–3.
Endo, B. Y. (1964). Penetration and development of Heterodera glycines in soybean roots and related anatomical changes. Phytopathology 54, 7988.
Endo, B. Y. (1978). Feeding plug formation in soybean roots infected with the soybean cyst nematode. Phytopathology 68, 1022–31.
Forrest, J. M. S., Trudgill, D. I., & Cotes, L. M. (1986). The fate of juveniles of Globodera rostochiensis and G. pallida in roots of susceptible and resistant potato cultivars with gene H1. Nematologica 32, 100–14.
Jones, M. G. K. (1981). The development and function of plant cells modified by endoparasitic nematodes. In Plant Parasitic Nematodes, Vol. 3 (ed. Zuckerman, B. M. and Rohde, R. H.), pp. 235–79. New York and London: Academic Press.
Muller, J., Rehbock, K. & Wyss, U. (1981). Growth of Heterodera schachtii with remarks on amounts of food consumed. Revue de Nematologie 4, 227–34.
Nie, N. H. (1983). SPSS Users Guide. New York: McGraw Hill.
Price, N. S. & Hague, N. G. M. (1981). The invasion of root tips of cereal by the cereal cyst nematode Heterodea avenae. Annals of Applied Biology 99, 301–6.
Raski, D. J. (1950). The life history and morphology of the sugar-beet nematode Heterodera schactii Schmidt. Phytopathology 40, 135–52.
Robinson, M., Atkinson, H. J. & Perry, R. N. (1986). The effect of delayed emergence on the subsequent infectivity of second stage juveniles of the potato cyst nematode Globodera rostochiensis. Nematologica 31, 171–8.
Robinson, M. P., Atkinson, H. J. & Perry, R. N. (1988). The association and partial characterisation of a fluorescent hypersensitive response of potato roots to the potato cyst nematodes Globodera rostochiensis and G. pallida. Revue de Nematologie 11, 99107.
Rumpenhorst, H. J. (1984). Intracellular feeding tubes associated with sedentary plant parasitic nematodes. Nematologica 30, 7085.
Widdowson, E., Doncaster, C. C. & Fenwick, D. W. (1958). Observations on the development of Heterodera rostochiensis Woll. in sterile root cultures. Nematologica 3, 308–14.
Wyss, U. & Zunke, U. (1986). Observations on the behaviour of second stage juveniles of Heterodera schactii within host roots. Revue de Nematologie 9, 153–65.

Keywords

Changes in nematode antigens recognized by monoclonal antibodies during early infections of soya beans with the cyst nematode Heterodera glycines

  • H. J. Atkinson (a1) and P. D. Harris (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed