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Antiprotozoal effects of metal nanoparticles against Ichthyophthirius multifiliis

  • MONA SALEH (a1), ABDEL-AZEEM ABDEL-BAKI (a2) (a3), MOHAMED A. DKHIL (a2) (a4), MANSOUR EL-MATBOULI (a1) and SALEH AL-QURAISHY (a2)...

Summary

Ichthyophthirius multifiliis is a widespread, ciliated protozoan ectoparasite of fish. In the present study, we investigated the effects of metal nanoparticles on the reproduction and infectivity of free-living stages of I. multifiliis. We determined that ~50% of theronts could be killed within 30 min of exposure to either 20 ng mL−1 gold, 10 ng mL−1 silver or 5 ng mL−1 zinc oxide nanoparticles. Silver and zinc oxide nanoparticles at concentration of 10 and 5 ng mL−1 killed 100 and 97% of theronts, respectively and inhibited reproduction of tomonts after 2 h exposure. Gold nanoparticles at 20 ng mL killed 80 and 78% of tomonts and theronts 2 h post exposure, respectively. In vivo exposure studies using rainbow trout (Oncoryhnchus mykiss) demonstrated that theronts, which survived zinc oxide nanoparticles exposure, showed reduced infectivity compared with control theronts. No mortalities were recorded in the fish groups cohabited with theronts exposed to either nanoparticles compared with 100% mortality in the control group. On the basis of the results obtained from this study, metal nanoparticles particularly silver nanoparticles hold the best promise for the development of effective antiprotozoal agents useful in the management of ichthyophthiriosis in aquaculture.

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This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

*Corresponding author: Clinical Division of Fish Medicine, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. E-mail: mona.saleh@vetmeduni.ac.at

References

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Allahverdiyev, A. M., Abamor, E. S., Bagirova, M., Ustundag, C., Kaya, C., Kaya, F. and Rafailovich, M. (2011). Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity underultraviolet light. International Journal of Nanomedicine 6, 27052714.
Antony, J. J., Nivedheetha, M., Siva, D., Pradeepha, G., Kokilavani, P., Kalaiselvi, S., Sankarganesh, A. and Balasundaram, A. (2013). Antimicrobial activity of Leucas aspera engineered silver nanoparticles against Aeromonas hydrophila in infected Catla catla . Colloids and Surfaces B: Biointerfaces 109, 2024.
Arora, S., Jain, J., Rajwade, J. M. and Paknikar, K. M. (2008). Cellular responses induced by silver nanoparticles: in vitro studies. Toxicology Letters 179, 93100.
Buzea, C., Blandino, I. I. P. and Robbie, K. (2007). Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2, 17172.
Cui, Y., Zhao, Y., Tian, Y., Zhang, W., , X. and Jiang, X. (2012). Themolecularmechanismof action ofbactericidal gold nanoparticles on Escherichia coli. Biomaterials 33, 23272333.
Daniel, S. C. G. K., Sironmani, T. A. and Dinakaran, S. (2016). Nano formulations as curative and protective agent for fish diseases: studies on red spot and white spot diseases of ornamental gold fish Carassius auratus . International Journal of Fisheries and Aquatic Studies 4, 255261.
Dickerson, H. W. and Findly, R. C. (2014). Immunity to Ichthyophthirius infections in fish: a synopsis. Developmental & Comparative Immunology 43, 290299.
Dizaj, S. M., Lotfipour, F., Barzegar-Jalali, M., Zarrintan, M. H. and Adibkia, K. (2014). Antimicrobial activity of the metals and metal oxide nanoparticles. Materials Science and Engineering C 44, 278284.
El Mahdy, M. M., Eldin, T. A. S., Aly, H. S., Mohammed, F. F. and Shaalan, M. I. (2015). Evaluation of hepatotoxic and genotoxic potential of silver nanoparticles in albino rats. Experimental and Toxicologic Pathology 67, 2129.
Farkas, J., Christian, P., Gallego-Urrea, J. A., Roos, N., Hassellov, M., Tollefsen, K. E. and Thomas, K. V. (2010). Effects of silver and gold nanoparticles on rainbowtrout (Oncorhynchus mykiss) hepatocytes. Aquatic Toxicoogy 96, 4452.
Franci, G., Falanga, A., Galdiero, S., Palomba, L., Rai, M., Morelli, G. and Galdiero, M. (2015). Silver nanoparticles as potential antibacterial agents. Molecules 20, 88568874.
Fu, Y. W., Zhang, Q. Z., Xu, D. H., Liang, J. H. and Wang, B. (2014). Antiparasitic effect of Cynatratoside C from Cynanchum atratum against Ichthyophthirius multifiliis on Grass Carp. Journal of Agricultural and Food Chemistry 62, 71837189.
Hoffman, G. L. (1967). Parasites of North American Freshwater Fishes. University California Press, Berkeley, Los Angeles, p. 486.
Hoffman, G. L. (1970). Intercontinental and transcontinental dissemination and transfaunation of fish parasites with emphasis on whirling disease (Myxosoma cerebralis). A Symposium on Diseases of Fishes and Shellfishes 6981.
Jiang, W., Mashayekhi, H. and Xing, B. (2009). Bacterial toxicity comparison between nano- and micro-scaled oxideparticles. Environmental Pollution 157, 16191625.
Kim, S. and Ryu, D. Y. (2013). Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues. Journal of Applied Toxicology 33, 7889.
Lara, H. H., Ayala-Núnez, N. V., Turrent, L. D. C. I. and Padilla, C. R. (2010). Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria. World Journal of Microbiology and Biotechnology 26, 615621.
Lima, E., Guerra, R., Lara, V. and Guzmán, A. (2013). Gold nanoparticles as efficient antimicrobial agents for Escherichia coli and Salmonella typhi . Chemistry Central Journal 7, 17.
Li, Q. L., Mahendra, S., Lyon, D. Y., Brunet, L., Liga, M. V., Li, D. and Alvarez, P. J. J. (2008). Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Research 42, 45914602.
Lolina, S. and Narayanan, V. (2013). Antimicrobial and anticancer activity of gold nanoparticles synthesized from grapes fruit extract. Chemical Science Transactions 2, 105110.
Mahanty, A., Mishra, S., Bosu, R., Maurya, U. K., Netam, S. P. and Sarkar, B. (2013). Phytoextracts-synthesized silver nanoparticles inhibit bacterial fish pathogen Aeromonas hydrophila . Indian Journal of Microbiology 53, 438446.
Neal, A. L. (2008). What can be inferred from bacterium nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles? Ecotoxicology 17, 362371.
Nigrelli, R. F., Pokorny, K. S. and Ruggieri, G. D. (1976): Notes on Ichthyophthirius multifiliis, a ciliate parasitic on fresh-water fishes, with some remarks on possible physiological races and spieces. Transactions of the American Microscopical Society 95, 607613.
Ramamoorthy, S., Kannaiyan, P., Moturi, M., Devadas, T., Muthuramalingam, J. and Natarajan, L., Arunachalam, N. and Ponniah, A. G. (2013). Antibacterial activity of zinc oxide nanoparticles against Vibrio harveyi . Indian Journal of Fisheries 60, 107112.
Rana, S. and Kalaichelvan, P. T. (2011). Antibacterial activities of metal nanoparticles. Advanced Biotechnology 11, 2123.
Rintamäki-Kinnunen, P. and Valtonen, E. T. (1997). Epizootiology of protozoans in farmed salmonids at northern latitudes. International Journal of Parasitology 27, 8999.
Saleh, M., Kumar, G., Abdel-Baki, A. A., Al-Quraishy, S. and El-Matbouli, M. (2016). In vitro antimicrosporidial activity of goldnanoparticles against Heterosporis saurida . BMC Veterinary Research 12, 44.
Schäperclaus, W. (1991). Diseases caused by ciliates. In Fish Diseases (ed. Schäperclaus, W., Kulow, H. and Schreckenbach, K.), pp. 702725. Amerind Publishing Co. Pvt. Ltd., New Delhi.
Schumacher, I. V., Wedekind, H. and El-Matbouli, M. (2011). Efficacy of quinine against ichthyophthiriasis common carp Cyprinus carpio . Diseases of Aquatic Organisms 95, 217224.
Shaalan, M., Saleh, M., El-Mahdy, M. and El-Matbouli, M. (2016). Recent progress in applications of nanoparticles in fish medicine: a review. Nanomedicine: Nanotechnology, Biology, and Medicine 12, 701710.
Shinn, A. P., Picón-Camacho, S. M., Bron, J. E., Conway, D., Yoon, G. H., Guo, F. C. and Taylor, N. G. (2012). The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Ciliophora). Veterinary Parasitology 186, 229236.
Soltani, M., Ghodratnema, M., Ahari, H., Ebrahimzadeh Mousavi, H. A., Atee, M., Dastmalchi, F. and Rahmanya, J. (2009). The inhibitory effect of silver nanoparticles on the bacterial fish pathogens. Streptococcus iniae, Lactococcus garvieae, Yersinia ruckeri and Aeromonas hydrophila. International Journal of Veterinary Research 3, 137142.
Stoimenov, P. K., Klinger, R. L., Marchin, G. L. and Klabunde, K. J. (2002). Metal oxide nanoparticles as bactericidal agents. Langmuir 18, 66796686.
Swain, P., Nayak, S. K., Sasmal, A., Behera, T., Barik, S. K., Swain, S. K., Mishra, S. S., Sen, A. K., Das, J. K. and Jayasankar, P. (2014). Antimicrobial activity of metal based nanoparticles against microbes associated with diseases in aquaculture. World Journal of Microbiology and Biotechnology 30, 24912502.
Tiwari, P. M., Vig, K., Dennis, V. A. and Singh, S. R. (2011). Functionalized gold nanoparticles and their biomedical applications. Nanomaterials 1, 3163.
Tucker, C. S. and Robinson, E. H. (1990). Channel Catfish Farming Handbook. Van Nostrand Reinhold, New York.
Umashankari, J., Inbakandan, D., Ajithkumar, T. T. and Balasubramanian, T. (2012). Mangrove plant, Rhizophora mucronata (Lamk, 1804) mediated one pot green synthesis of silver nanoparticles and its antibacterial activity against aquatic pathogens. Aquatic Biosystems 8, 11.
Vaseeharan, B., Ramasamy, P. and Chen, J. C. (2010). Antibacterial activity of silver nanoparticles (AgNps) synthesized by tea leaf extracts against pathogenic Vibrio harveyi and its protective efficacy on juvenile Feneropenaeus indicus . Letters in Applied Microbiology 50, 352356.
Velmurugan, P., Iydroose, M., Lee, S. M., Cho, M., Park, J. H., Balachandar, V. and Oh, B. (2014). Synthesis of silver and gold nanoparticles using cashew nut shell liquid and its antibacterial activity against fish pathogens. Indian Journal of Microbiology 54, 196202.
Wang, H., Qiao, X., Chen, J., Wang, X. and Ding, S. (2005). Mechanisms of PVP in the preparation of silver nanoparticles. Materials Chemistry and Physics 94, 449453.
Zhang, L., Jiang, Y., Ding, Y., Povey, M. and York, D. (2007). Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (Nanofluids). Journal of Nanoparticle Research 9, 479489.
Zhang, L., Jiang, Y., Ding, Y., Daskalakis, N., Jeuken, L., Povey, M., O'Neill, A. J. and York, D. W. (2010). Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. Coli . Journal of Nanoparticle Research 12, 16251636.
Zhou, Y., Kong, Y., Kundu, S., Cirillo, J. D. and Liang, H. (2012). Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. Journal of Nanobiotechnolology 10, 19.

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Antiprotozoal effects of metal nanoparticles against Ichthyophthirius multifiliis

  • MONA SALEH (a1), ABDEL-AZEEM ABDEL-BAKI (a2) (a3), MOHAMED A. DKHIL (a2) (a4), MANSOUR EL-MATBOULI (a1) and SALEH AL-QURAISHY (a2)...

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