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Antigenic variation in cyclically transmitted Trypanosoma brucei. Variable antigen type composition of metacyclic trypanosome populations from the salivary glands of Glossina morsitans

Published online by Cambridge University Press:  06 April 2009

S. L. Hajduk ,
Cathy R. Cameron ,
J. D. Barry  and
K. Vickerman
S. L. Hajduk
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
Cathy R. Cameron
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
J. D. Barry
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
K. Vickerman
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
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Summary

Tsetse flies (Glossina morsitans) were fed on the blood of mice containing any one of 5 variable antigen types (VATs) of Trypanosoma brucei AnTAR 1 serodeme. The VATs of the metacyclic trypanosomes subsequently detected in the flies' saliva probes were investigated using monospecific antisera to AnTAR 1 VATs in indirect immunofluorescence and trypanolysis reactions; these sera included 3 raised against AnTats 1.6, 1.30 and 1.45, previously identified as components of the metacyclic population (M-VATs), and against the 5 VATs originally ingested by the flies. The percentage of metacyclics reacting with a particular M-VAT antiserum remained more or less constant (AnTat 1.6, 6·0–8·3%; AnTat 1.30, 13·7–18·2%; AnTat 1.45, 2·0–8·0%), regardless of the age of the fly or the ingested VAT. As these 3 VATs accounted for no more than 30% of the metacyclic population, the existence of at least one more VAT is envisaged. The ingested VAT could not be detected among the AnTAR 1 metacyclic trypanosomes.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 3 , December 1981 , pp. 595 - 607
Copyright
Copyright © Cambridge University Press 1981

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References

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