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An automated large-scale culture system of Plasmodium falciparum using tangential flow filtration for medium change

Published online by Cambridge University Press:  06 April 2009

T. Ponnudurai ,
A. H. W. Lensen  and
J. H. E. Th. Meuwissen
T. Ponnudurai
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
A. H. W. Lensen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
J. H. E. Th. Meuwissen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
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Summary

Asexual stages and mature gametocytes of Plasmodium falciparum have been produced in a 500 ml suspension culture system containing 6–10% red cells. Medium change was automated and was accomplished using a tangential flow filtration unit. The rate of multiplication of the parasite (isolate NF 54) was consistently high when compared with static cultures and ranged from 6·4 × to 15·3 × per cycle in 8 experiments. Mature gametocytes were present in up to 2% of red cells in 14-day-old cultures. Only minor modifications would be required to further scale-up the volume of the culture.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 3 , December 1983 , pp. 439 - 445
Copyright
Copyright © Cambridge University Press 1983

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References

REFERENCES

Butcher, G. A. (1981 a). A comparison of static thin layer and suspension cultures for the maintenance in vitro of Plasmodium falciparum. Annals of Tropical Medicine and Parasitology 75, 7–17.CrossRefGoogle ScholarPubMed
Butcher, G. A. (1981 b). A simple automated device for the maintenance of Plasmodium falciparum in suspension culture. Annals of Tropical Medicine and Parasitology 75, 111–13.CrossRefGoogle ScholarPubMed
Butcher, G. A. (1982). The behaviour of different strains of Plasmodium falciparum in suspension and static culture. Transactions of the Royal Society of Tropical Medicine and Hygiene 76, 407–9.CrossRefGoogle ScholarPubMed
Palmer, K. L., Hui, G. S. N. & Siddiqui, W. A. (1982). A large-scale in vitro production system for Plasmodium falciparum. Journal of Parasitology 68, 1180–3.CrossRefGoogle ScholarPubMed
Ponnudurai, T., Meuwissen, J. H. E. Th., Leeuwėnberg, A. D. E. M., Verhave, J. P. & Lensen, A. H. W. (1982 a). The production of mature gametocytes of Plasmodium falciparum in continuous cultures of different isolates infective to mosquitoes. Transactions of the Royal Society of Tropical Medicine and Hygiene 76, 242–50.CrossRefGoogle ScholarPubMed
Ponnudurai, T., Lensen, A. H. W., Leeuwenberg, A. D. E. M. & Meuwissen, J. H. E. Th. (1982 b). Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene 76, 812–18.CrossRefGoogle ScholarPubMed
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.CrossRefGoogle ScholarPubMed
Zolg, J. W., Macleod, A. J., Dickson, I. H. & Scaife, J. G. (1982). Plasmodium falciparum: modifications of the in vitro culture conditions improving parasitic yields. Journal of Parasitology 68, 1072–80.CrossRefGoogle ScholarPubMed