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An automated large-scale culture system of Plasmodium falciparum using tangential flow filtration for medium change

Published online by Cambridge University Press:  06 April 2009

T. Ponnudurai ,
A. H. W. Lensen  and
J. H. E. Th. Meuwissen
T. Ponnudurai
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
A. H. W. Lensen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
J. H. E. Th. Meuwissen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
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Summary

Asexual stages and mature gametocytes of Plasmodium falciparum have been produced in a 500 ml suspension culture system containing 6–10% red cells. Medium change was automated and was accomplished using a tangential flow filtration unit. The rate of multiplication of the parasite (isolate NF 54) was consistently high when compared with static cultures and ranged from 6·4 × to 15·3 × per cycle in 8 experiments. Mature gametocytes were present in up to 2% of red cells in 14-day-old cultures. Only minor modifications would be required to further scale-up the volume of the culture.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 3 , December 1983 , pp. 439 - 445
Copyright
Copyright © Cambridge University Press 1983

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References

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