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Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China

Published online by Cambridge University Press:  10 July 2018

Nan Liang
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China College of Animal Husbandry Engineering, Henan Vocational College of Agriculture, Zhongmu 451450, Henan Province, China
Yayun Wu
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Mingfei Sun
Affiliation:
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Yankai Chang
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Xuhui Lin
Affiliation:
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Linzeng Yu
Affiliation:
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Suhui Hu
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Xiangqian Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Shuangjian Zheng
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Zhaohui Cui
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Longxian Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, China International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, Henan Province, China
Corresponding

Abstract

To determine the prevalence of Cryptosporidium in dairy cattle in Guangdong Province, South China, 1440 fecal samples were collected from 10 farms and screened for Cryptosporidium with PCR. The overall prevalence of Cryptosporidium was 4.38% (63/1440), and the infection rates in preweaned calves, postweaned calves, heifers and adults were 6.4% (19/297), 6.19% (33/533), 1.48% (4/271) and 2.06% (7/339), respectively. Three Cryptosporidium species, Cryptosporidium andersoni (n = 33), Cryptosporidium bovis (n = 22) and Cryptosporidium ryanae (n = 8) were detected by DNA sequence analysis of the 63 positive samples, and C. andersoni was identified as the most common species on the dairy cattle farms. In preweaned calves, C. bovis was the most prevalent species (9/19, 47.4%). In contrast, C. andersoni was the predominant species (19/33, 57.6%) in postweaned calves and the only species found in heifers and adults. The zoonotic species Cryptosporidium parvum was not detected in this study. Twenty-four C. andersoni isolates were successfully classified into three multilocus sequence typing (MLST) subtypes. MLST subtype A4,A4,A4,A1 was the predominant subtype, and MLST subtype A2,A5,A2,A1, previously found in sheep, was detected in cattle for the first time. A linkage disequilibrium analysis showed that the C. andersoni isolates had a clonal genetic population structure. However, further molecular studies are required to better understand the epidemiology of Cryptosporidium in Guangdong.

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Research Article
Copyright
Copyright © Cambridge University Press 2018 

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Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China
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