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Identification of the dominant cyst antigens of Toxoplasma gondii

Published online by Cambridge University Press:  06 April 2009

G. Woodison  and
J. E. Smith
G. Woodison
Affiliation:
Department of Pure and Applied Biology, Agricultural Sciences Building, University of Leeds, Leeds LS2 3PZ
J. E. Smith
Affiliation:
Department of Pure and Applied Biology, Agricultural Sciences Building, University of Leeds, Leeds LS2 3PZ
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Summary

Brain cysts from three strains of Toxoplasma gondii were purified and analysed by SDS-PAGE and immunoblotting. Western blot analysis revealed the presence of major cyst/bradyzoite antigens at Mr 25000, 36000 and 67000 common to all strains and recognized by many individual sera. There was also evidence of strain variation in cyst antigens with, for example, antigens at Mr 88000, 28000 and 26000 unique to the 17025 strain. There was some overlap in the molecular weight of cyst and tachyzoite antigens, but the major tachyzoite surface antigen (P30) could not be detected in brain cysts.

Keywords

Toxoplasmabradyzoitecyst antigens
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 389 - 392
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Cornelissen, A. W. C. A., Overdulve, J. P. & Hoenderboom, J. M. (1981). Separation of Isospora (Toxplasna gondii) cysts and cystozoites from mouse brain by continuous density-gradient centrifugation. Parasitology 83, 103–8.CrossRefGoogle ScholarPubMed
Darcy, F., Deslee, D., Santoro, F., Charif, H., Auriault, C., Decoster, A., Duquesne, V. & Capron, A. (1988). Induction of a protective antibody-dependent response against toxoplasmosis by in vitro excreted/secreted antigens from tachyzoites of Toxoplasma gondii. Parasite Immunology 10, 553–67.Google Scholar
Kasper, L. H. (1989). Identification of stage-specific antigens of Toxoplasma gondii. Infection and Immunity 57, 668–72.Google Scholar
Kasper, L. H., Bradley, M. S. & Pfefferkorn, E. R. (1984). Indentification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. Journal of Immunology 132, 443–9.CrossRefGoogle Scholar
Kimata, I. & Tanabe, K. (1987). Secretion by Toxoplasma gondii of an antigen that appears to become associated with the parasitophorous vacuole membrane upon invasion of the host cell. Journal of Cell Science 88, 231–9.Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Luft, B. J., Brooks, R. G., Conley, F. K., McCabe, R. E. & Remington, J. S. (1984). Toxoplasmic encephalitis in patients with acquired immune deficiency syndrome. Journal of the American Medical Association 252, 913–17.Google Scholar
Lunde, M. N. & Jacobs, L. (1983). Antigenic differences between endozoites and cystozoites of Toxoplasma gondii. Journal of Parasitology 69, 806–11.Google Scholar
Omata, Y., Igarashi, M., Ramos, M. I. & Nakabayashi, T. (1989). Toxoplasma gondii: Antigenic differences between endozoites and cystozoites defined by monoclonal antibodies. Parasitology Research 75, 189–93.Google Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Science, USA 76, 4350–4.Google Scholar
Ware, P. L. & Kasper, L. H. (1987). Strain-specific antigens of Toxoplasma gondii. Infection and Immunity 55, 778–83.CrossRefGoogle ScholarPubMed