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Molecular identification and detection of Eutypa lata in grapevine

Published online by Cambridge University Press:  12 July 2005

Richard LARDNER ,
Belinda E. STUMMER ,
Mark R. SOSNOWSKI  and
Eileen S. SCOTT
Richard LARDNER
Affiliation:
Cooperative Research Centre for Viticulture, P.O. Box 154, Glen Osmond, SA 5064, Australia. E-mail: richard.lardner@adelaide.edu.au Discipline of Plant and Pest Science, University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia.
Belinda E. STUMMER
Affiliation:
Cooperative Research Centre for Viticulture, P.O. Box 154, Glen Osmond, SA 5064, Australia. E-mail: richard.lardner@adelaide.edu.au Discipline of Plant and Pest Science, University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia.
Mark R. SOSNOWSKI
Affiliation:
Cooperative Research Centre for Viticulture, P.O. Box 154, Glen Osmond, SA 5064, Australia. E-mail: richard.lardner@adelaide.edu.au South Australian Research and Development Institute (SARDI), GPO Box 397, Adelaide, SA 5001, Australia.
Eileen S. SCOTT
Affiliation:
Cooperative Research Centre for Viticulture, P.O. Box 154, Glen Osmond, SA 5064, Australia. E-mail: richard.lardner@adelaide.edu.au Discipline of Plant and Pest Science, University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia.
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Abstract

Eutypa lata, the causal agent of Eutypa dieback of grapevines, is difficult to identify on the basis of colony morphology and is often out-competed by other fungi when isolated from wood. To facilitate diagnosis of the pathogen, we designed SCAR primers capable of amplifying DNA of E. lata and constructed a genomic DNA library from which DNA sequences specific to E. lata were identified and sequenced. SCAR primers were used to identify E. lata directly from culture without the requirement for DNA extraction or prolonged incubation periods and could also detect the pathogen in DNA isolated from grapevine wood. RFLP probes were used in slot-blot assays to detect the pathogen in DNA isolated from 1 yr old cane as well as from mature grapevine trunks. The markers developed in this study have the potential to be used as a research tool to gather information on the epidemiology of the disease and to assess the efficacy of potential control agents against E. lata.

Type
Research Article
Information
Mycological Research , Volume 109 , Issue 7 , July 2005 , pp. 799 - 808
Copyright
© The British Mycological Society 2005

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