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De novo synthesis is involved in the production of extracellular α-amylase activity from Thermomyces lanuginosus in the stationary phase

Published online by Cambridge University Press:  10 July 2002

Steen ARNESEN ,
Susanne H. ERIKSEN ,
Jørgen OLSEN  and
Bo JENSEN
Steen ARNESEN
Affiliation:
Department of General Microbiology, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83H, DK-1307 K, Copenhagen, Denmark.
Susanne H. ERIKSEN
Affiliation:
Statens Serum Institut, Artillerivej 5, DK-2300 S, Copenhagen, Denmark.
Jørgen OLSEN
Affiliation:
Department of General Microbiology, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83H, DK-1307 K, Copenhagen, Denmark.
Bo JENSEN
Affiliation:
Department of General Microbiology, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83H, DK-1307 K, Copenhagen, Denmark.
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Abstract

The thermophilic fungus Thermomyces lanuginosus was cultivated in shake-flasks for up to 120 h with low-molecular-weight dextran as carbon source. Maximal biomass was attained after 48 h of growth whereas extracellular α-amylase activity was highest in the stationary phase with maximum activity after 96 h of cultivation. A similar pattern was found for total extracellular protein. Pulse-labeling of proteins showed that α-amylase and other unidentified proteins were de novo synthesised in the stationary phase. Using specific primers with sequences from α-amylase from T. lanuginosus, RT-PCR analysis showed that α-amylase transcription did not start until late in the growth phase and reached a maximum more than 24 h after maximal biomass was obtained.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 3 , March 2002 , pp. 345 - 348
Copyright
© The British Mycological Society 2002

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