Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-07-06T05:03:54.827Z Has data issue: false hasContentIssue false

Beauveria bassiana haplotype determination based on nuclear rDNA internal transcribed spacer PCR–RFLP

Published online by Cambridge University Press:  26 February 2002

Brad S. COATES
Affiliation:
USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, c/o Insectary, Iowa State University, Ames, IA 50011, USA. Interdepartmental Genetics Program, Iowa State University, Ames, IA 50011, USA. Department of Entomology, Iowa State University, Ames IA 50011, USA.
Richard L. HELLMICH
Affiliation:
USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, c/o Insectary, Iowa State University, Ames, IA 50011, USA. Department of Entomology, Iowa State University, Ames IA 50011, USA.
Leslie C. LEWIS
Affiliation:
USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, c/o Insectary, Iowa State University, Ames, IA 50011, USA. Department of Entomology, Iowa State University, Ames IA 50011, USA.
Get access

Abstract

DNA sequence alignment of the nuclear 5·8S ribosomal RNA (rRNA) gene and internal transcribed spacers (ITS) from Beauveria bassiana demonstrated that 6·62% sequence variation existed between nine isolates. A higher level of mutation was observed within the ITS regions, where 8·39% divergence occurred. Polymerase chain reaction restriction fragment length polymorphism, PCR–RFLP, and DNA sequence alignment of the ITS1 and ITS2 regions identified seven polymorphic restriction endonuclease sites, Alu I, Hha I, Hinf I, Sin I, Tru 9AI and two Tha I restriction sites. The allelic frequency of each genetic marker was determined from 96 isolates. PCR–RFLP defined 24 B. bassiana genotypes within the sample set, from which eight phylogenetic clusters were predicted to exist. AMOVA and Fst (θ) indicated that no significant correlation existed between B. bassiana haplotype and insect host range as defined by insect order from which each isolate was derived.

Type
Research Article
Copyright
© The British Mycological Society 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)