The interaction of the electron beam with materials during TEM/STEM imaging often leads to radiation damage. While a variety of low-dose techniques can help mitigate beam damage, true dose management starts with knowing the precise total accumulated dose and dose rate that a sample has seen throughout an experiment. AXON Dose allows users to calibrate their instruments, track electron dose/dose rate across a sample as a function of time and location, and quantify the impact of dose on individual samples.

Super-resolution microscopy gives researchers invaluable opportunities and continues to make great strides in terms of performance and applicability. Clever developments in stimulated emission depletion (STED) microscopy have pushed the doors open wider for many applications. Here, we discuss three examples: first, how time-resolved detection unlocks new information; then, live-cell imaging enabled by intelligent illumination schemes; and finally, deep tissue imaging with dynamic aberration correction. As an outlook, we examine MINFLUX as an approach for molecular resolution with fluorescence.

As part of the Hitachi High-Tech America Inspire STEM Education Outreach Programme in the UK, a successful STEM (Science, Technology, Engineering and Math) education pilot has seen over 5000 students from 80 schools given the opportunity to use scanning electron microscopes (SEMs) for their own experiments. The collaborative project allowed students to design, propose, and execute experiments to give them hands-on experience with scientific research. Feedback from the project has been resoundingly positive, with many students gaining new skills and an interest in STEM, and it looks set to grow further to allow more students access to high-level scientific equipment.

Many universities, commercial companies, and science organizations who use microscopy have outreach programs. The members of such generally love to share their enthusiasm and passion with the next generation. Content and confidence on providing such a program often comes from sharing ideas with like-minded scientists. These programs are typically delegated with the acronym STEM or STEAM: science, technology, engineering, art, and math. One of MSA's outreach programs is ProjectMICRO. Included are some STEAM ideas to share.