Skip to main content Accessibility help
×
Home

ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations

  • Taewoo Kim (a1), Eugene Serabyn (a2), Maximilian Schadegg (a3), Kurt Liewer (a2), Nathan Oborny (a2), J. Kent Wallace (a2), Stephanie Rider (a1), Manuel Bedrossian (a1), Christian Lindensmith (a2) and Jay Nadeau (a3)...

Abstract

This is the first of two articles on the Extant Life Volumetric Imaging System (ELVIS) describing a combined digital holographic microscope (DHM) and a fluorescence light-field microscope (FLFM). The instrument is modular and robust enough for field use. Each mode uses its own illumination source and camera, but both microscopes share a common objective lens and sample viewing chamber. This allows correlative volumetric imaging in amplitude, quantitative phase, and fluorescence modes. A detailed schematic and parts list is presented, as well as links to open-source software packages for data acquisition and analysis that permits interested researchers to duplicate the design. Instrument performance is quantified using test targets and beads. In the second article on ELVIS, to be published in the next issue of Microscopy Today, analysis of data from field tests and images of microorganisms will be presented.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations
      Available formats
      ×

Copyright

Corresponding author

References

Hide All
[1]Olson, RJ and Sosik, HM, Limnol Oceanogr 5 (2007) 195203.10.4319/lom.2007.5.195
[2]Dickinson, I et al. , in Their World: A Diversity of Microbial Environments, Vol. 1, ed. Hurst, CJ, Springer, 2016, 341–81.10.1007/978-3-319-28071-4_9
[3]Marshall, WF et al. , BMC Biol 10 (2012) 101–23.
[4]Wallace, JK et al. , Optics Express 23 (2015) 17367–78.10.1364/OE.23.017367
[5]Serabyn, G et al. , in 2019 IEEE Aerospace Conference 10.1109/AERO.2019.8741627 (2019) 17.
[6]Cuche, E et al. , Interferometry in Speckle Light (2000) 213–18.10.1007/978-3-642-57323-1_26
[7]Marquet, P et al. , Neurophotonics 1 (2014) 020901.10.1117/1.NPh.1.2.020901
[8]Lee, K et al. , Sensors 13 (2013) 4170–91.10.3390/s130404170
[9]Pavillon, N et al. , J Biophoton 3 (2010) 432–36.
[10]Quan, X et al. , Opt Rev 22 (2015) 349–53.10.1007/s10043-015-0042-4
[11]Broxton, M et al. , Optics Express 21 (2013) 25418–39.10.1364/OE.21.025418
[12]Prevedel, R et al. , Nature Meth 11 (2014) 727–30.10.1038/nmeth.2964
[13]Serabyn, E, Appl Optics 58 (2019) A27382.
[14]Bolan, J et al. , SoftwareX 5 (2016) 101–06.
[15]Cohoe, D et al. , Front Physics 7 (2019) 94.10.3389/fphy.2019.00094
[16]Hand, KP et al. , Project Engineering Team Report of the Europa Lander Science Definition Team (2017) https://europa.nasa.gov/resources/58/europa-lander-study-2016-report.

Keywords

ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations

  • Taewoo Kim (a1), Eugene Serabyn (a2), Maximilian Schadegg (a3), Kurt Liewer (a2), Nathan Oborny (a2), J. Kent Wallace (a2), Stephanie Rider (a1), Manuel Bedrossian (a1), Christian Lindensmith (a2) and Jay Nadeau (a3)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.