If you are an optical microscopist, chances are you have sometimes wished for a way to increase the depth of focus of your images. In this article I describe a method that does this using a simple combination of functions built into most image processing software - so it will not cost you very much to try, The method, however, is not universal and some trial-and-error will be required to get it to work in different applications. Finally, this method is not limited to microscopy and will work with any imaging system.

The basic steps for creating an image with a large depth of focus are straight forward:

1. 1. Take a series of images at different focal planes

2. 2. Remove the blurred content from each image

3. 3. Combine the debiurred images into a single image