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Lichen elements as pollution indicators: evaluation of methods for large monitoring programmes

  • Susan WILL-WOLF (a1), Sarah JOVAN (a2) and Michael C. AMACHER (a3)


Lichen element content is a reliable indicator for relative air pollution load in research and monitoring programmes requiring both efficiency and representation of many sites. We tested the value of costly rigorous field and handling protocols for sample element analysis using five lichen species. No relaxation of rigour was supported; four relaxed protocols generated data significantly different from rigorous protocols for many of the 20 validated elements. Minimally restrictive site selection criteria gave quality data from 86% of 81 permanent plots in northern Midwest USA; more restrictive criteria would likely reduce indicator reliability. Use of trained non-specialist field collectors was supported when target species choice considers the lichen community context. Evernia mesomorpha, Flavoparmelia caperata and Physcia aipolia/stellaris were successful target species. Non-specialists were less successful at distinguishing Parmelia sulcata and Punctelia rudecta from lookalikes, leading to few samples and some poor quality data.



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Bargagli, R. & Mikhailova, I. (2002) Accumulation of inorganic contaminants. In Monitoring with Lichens – Monitoring Lichens. NATO Science Series (P. L. Nimis, C. Scheidegger & P. Wolseley, eds): 6584. The Hague: Kluwer Academic Publishers.
Boltersdorf, S. H., Pesch, R. & Werner, W. (2014) Comparative use of lichens, mosses and tree bark to evaluate nitrogen deposition in Germany. Environmental Pollution 189: 4353.
Community Modeling and Analysis System (CMAS) Center (2015) Community multi-scale air quality (CMAQ) project. Version 5.1. November 2015 release. (Open-source development project hosted by U.S. EPA Atmospheric Science Modeling Division) Accessed November 2016.
Donovan, G. H., Jovan, S. E., Gatziolis, D., Burstyn, I., Michael, Y. L., Monleon, V. J. & Amacher, M. C. (2016) Using an epiphytic moss to identify previously unknown sources of atmospheric cadmium pollution. Science of the Total Environment 569: 8493.
Ferry, B. W., Baddeley, M. S. & Hawksworth, D. L. (eds) (1973) Air Pollution and Lichens. Toronto: University of Toronto Press.
Forbes, P. B. C., van der Wat, L. & Kroukamp, E. M. (2015) Biomonitors. In Monitoring of Air Pollutants: Sampling, Sample Preparation, and Analytical Techniques. Comprehensive Analytical Chemistry. Vol. 70 (P. B. C. Forbes, ed.): 53108. Amsterdam: Elsevier.
Gailey, F. A. Y. & Lloyd, O. L. (1986) Methodological investigations into low technology monitoring of atmospheric metal pollution: part 3 – the degree of replicability of the metal concentrations. Environmental Pollution (Series B) 12: 85109.
Gatziolis, D., Jovan, S., Donovan, G., Amacher, M. & Monleon, M. (2016) Elemental atmospheric pollution assessment via moss-based measurements in Portland, Oregon. General Technical Report PNW-GTR-938. Portland, Oregon: US Department of Agriculture, Forest Service, Pacific Northwest Research Station. Available at: (Accessed September 2016).
Geiser, L. H. & Neitlich, P. (2007) Air pollution and climate gradients in western Oregon and Washington indicated by epiphytic macrolichens. Environmental Pollution 145: 203218.
Halleraker, J. H., Reimann, C., de Caritat, P., Finne, T. E., Kashulina, G., Niskaavaara, H. & Bogatyrev, I. (1998) Reliability of moss (Hylocomium splendens and Pleurozium schreberi) as a bioindicator of atmospheric chemistry in the Barents region: interspecies and field duplicate variability. Science of the Total Environment 218: 123139.
Heller-Zeisler, S. F., Zeisler, R., Zeiller, E., Parr, R. M., Radecki, Z., Burns, K. I. & De Regge, P. (1999) Report on the intercomparison run for determination of trace and minor elements in lichen material IAEA-336. Vienna: International Atomic Energy Agency. Available at: (Accessed May 2016).
Jovan, S. & McCune, B. (2006) Using epiphytic macrolichen communities for biomonitoring ammonia in forests of the greater Sierra Nevada, California. Water, Air, and Soil Pollution 170: 6993.
Jovan, S., Riddell, J., Padgett, P. E. & Nash, T. H. III (2012) Eutrophic lichens respond to multiple forms of N: implications for critical levels and critical loads research. Ecological Applications 22: 19101922.
Kansanen, P. H. & Venetvaara, J. (1991) Comparison of biological collectors of airborne heavy metals near ferrochrome and steel works. Water, Air and Soil Pollution 60: 337359.
Karakas, S. Y. & Tuncel, S. G. (2004) Comparison of accumulation capacities of two lichen species analyzed by instrumental neutron activation analysis. Journal of Radioanalytical and Nuclear Chemistry 259: 113118.
Loppi, S., Faleri, C. & Paoli, L. (2014) Influence of sample cleaning prior to the analysis on the elemental content of the lichen Xanthoria parietina (L.) Th. Fr. Bulletin of Environmental Contamination and Toxicology 93: 350353.
Markert, B., Wappelhorst, O., Weckert, V., Herpin, U., Siewers, U., Friese, K. & Breulmann, G. (1999) The use of bioindicators for monitoring the heavy-metal status of the environment. Journal of Radioanalytical and Nuclear Chemistry 240: 425429.
Martin, M. H. & Coughtrey, P. J. (1982) Biological Monitoring of Heavy Metal Pollution. London: Applied Science Publishers.
McMurray, J. A., Roberts, D. W., Fenn, M. E., Geiser, L. H. & Jovan, S. (2013) Using epiphytic lichens to monitor nitrogen deposition near natural gas drilling operations in the Wind River Range, WY, USA. Water, Air and Soil Pollution 224: 14871501.
National Acidic Deposition Program (NADP) (2016) Total deposition maps. Available at: (Accessed November 2016).
Paoli, L., Guttová, A., Grassi, A., Lackovicová, A., Senko, D. & Loppi, S. (2014) Biological effects of airborne pollutants released during cement production assessed with lichens (SW Slovakia). Ecological Indicators 40: 127135.
Purvis, O. W., Chimonides, P. J., Jeffries, T. E., Jones, G. C., Read, H. & Spiro, B. (2005) Investigating biogeochemical signatures in the lichen Parmelia sulcata at Burnham Beeches, Buckinghamshire, England. Lichenologist 37: 329344.
Root, H. T., Geiser, L. H., Fenn, M. E., Jovan, S., Hutten, M. A., Ahuja, S., Dillman, K., Schirokauer, D., Berryman, S. & McMurray, J. A. (2013) A simple tool for estimating throughfall nitrogen deposition in forests of western North America using lichens. Forest Ecology and Management 306: 18.
Sloof, J. E. & Wolterbeek, B. T. (1991) National trace-element air pollution monitoring survey using epiphytic lichens. Lichenologist 23: 139165.
SPSS (2015) IBM SPSS Statistics. Release 1989–2015.
United States Environmental Protection Agency (US EPA) (2016) Air data: air quality data collected at outdoor monitors across the US. Available at: (Accessed November 2016).
van Haluwyn, C. & van Herk, C. M. (2002) Bioindication: the community approach. In Monitoring with Lichens – Monitoring Lichens (P. L. Nimis, C. Scheidegger & P. Wolseley, eds): 3964. Dordrecht: Kluwer Academic Publishers.
Will-Wolf, S., Nelsen, M. P., Trest, M. T., Rolih, K., Reis, A. & Jovan, S. (2014) Lichen community indices for response to climate and air quality in the Mid-Atlantic states, U.S.A. St. Paul, Minnesota: Internal USDA FIA report, October 2014.
Will-Wolf, S., Makholm, M. M., Nelsen, M. P., Trest, M. T., Reis, A. & Jovan, S. (2015) Element analysis of two common macrolichens supports bioindication of air pollution and lichen response in rural midwestern U.S.A. Bryologist 118: 5982.
Will-Wolf, S., Jovan, S. & Amacher, M. A. (2017 a) Lichen elemental content bioindicators for air quality in upper Midwest, USA: a model for large-scale monitoring. Ecological Indicators 78: 253263.
Will-Wolf, S., Jovan, S. & Amacher, M. C. (2017 b) Lichen elemental indicators for air pollution in eastern U. S. A. forests; a pilot study in the upper Midwest. USDA Forest Service, Pacific Northwest Research Station, General Technical Report (in press).
Yemets, O. A., Solhaug, K. A. & Gauslaa, Y. (2014) Spatial dispersal of airborne pollutants and their effects on growth and viability of lichen transplants along a rural highway in Norway. Lichenologist 46: 809823.


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