The efficiency of nitrogen fertilizer use is as low as 0·30–0·35 in most agricultural practices due to losses to the natural environment in many ways. Ammonia (NH3) volatilization is important in paddy rice fields. The present study investigated the effect of a surface film-forming material (SFFM) on NH3 volatilization from a paddy rice field, by model estimation using parameters from evaporative pan experiments. The modified Jayaweera–Mikkelsen (J-M) ammonia volatilization model was employed to simulate and elucidate the effect of SFFM in reducing ammonia volatilization from paddy fields. A parameter of kf value in the J–M modified model was introduced to represent the resistance of NH3 volatilization loss from SFFM. The simulated data fitted well with the prediction of the J–M modified model and field observations. The modified J–M model is suitable for screening and verifying the new SFFM, and merits further study.

A simulated grazing system was set up in Huanxian County, Gansu province, on the Loess Plateau of Northwest China, involving the purchase of 18-month-old wether lambs in June of each year at c. 20 kg body weight (BW) and sale 6 months later at c. 35 kg BW. Three stocking rate (SR) treatments of 2·7, 5·3 and 8·7 wether lambs/ha were evaluated on geographically separated warm season (WS) and cold season (CS) paddocks c. 1 km apart; 3 years' data are reported (2004–2006). The metabolizable energy (ME) yield of the grazing system, calculated from the weight of animals fed and their weight gain, averaged 1·7, 3·3 and 4·7 GJ/ha/year for 2004, 2005 and 2006, respectively, in the WS and 0·9, 1·9, and 2·7 GJ/ha/year, respectively, in the CS for SR of 2·7, 5·3 and 8·7 animals/ha. Detailed grazing behaviour records were kept in order to elucidate intake dynamics. In these grazing systems, bite weight was typically c. 0·04 g/bite, lower than for temperate grazing systems at comparable herbage mass. A hypothesis for further study is proposed that this may relate to the distribution of a similar herbage mass over a greater sward height range in steppe vegetation than in temperate grass pasture. Sheep increased their bite rate (bites/min) and the number of steps/min at higher SR to compensate, such that intake/animal was reduced by not more than 10% with a threefold increase in SR. At higher SR, herbage ground cover on grazed plots was still lower than on ungrazed plots, 1 year after a 90-day summer grazing period or a 48-day winter grazing period. The significance of the findings for management of these systems is briefly discussed.

Agricultural activity involves direct interaction with the physical environment factors in the environmental context in which the activity is developed. Galicia, northwest Spain, is an environmentally heterogeneous region that encompasses territorial spaces with different levels of suitability for each agricultural activity. In barely 30 years, the production systems of the region have evolved from self-sufficiency to commercial production; however, the requirements of production for each differ greatly. During such a transformation, many farms have disappeared while other farms have conformed to the requirements of the current production systems without changing location. Decision-making in rural planning requires knowing the spatial distribution of farms, the evolution of farm distribution and the relationship between the spatial location of farms (associated with some specific environmental characteristics) and the production systems used.

The current paper describes a methodology for analysing the spatial distribution of farms and for determining the relationships between the spatial location of farms and the values of the physical environment factors that are characteristic of each spatial location. The methodology has been developed by using Agricultural Census data and is exemplified through the analysis of two crops (wheat and potato) and one farming activity (dairy farming). Results show the location of farms and the production systems used, and reveal different degrees of fit to the physical environment factors considered.

Field experiments (15 years) were carried out to study the effects of no-tillage (NT) and conventional tillage (CT) management practices on the soil chemical properties, microbial biomass, soil enzymatic activities and winter wheat yield on a cinnamon soil in Shanxi, on the Chinese Loess Plateau. Compared to CT, NT increased soil organic carbon, soil total nitrogen and soil total phosphorus in the 0–100 mm layer by 25, 18 and 7%, respectively. Microbial biomass C and N contents under NT were 41 and 57% greater than under CT on the same layer. In general, higher enzymatic activities were found in the more superficial layers of soil under NT than under CT in the same layer. Winter wheat yield was c. 20% higher under NT than under CT. These findings have implications for understanding how conservation tillage practices improve soil quality and sustainability in the rainfed dryland farming areas of northern China.

The current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used for in situ and in vivo experiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively. In situ effective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production, in vitro and in vivo determined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.

Root system senescence and nitrogen (N) release from red clover (Trifolium pratense L.) plants, grown under semi-sterile conditions and a controlled environment, were studied for 28 days following temporary or prolonged abiotic stress. Plants stressed temporarily, to simulate grazing, recovered with no additional N lost in leachate. In contrast, plants subjected to prolonged stress that simulated overwintering conditions and inhibited shoot re-growth survived stress lasting 7 days, but plant viability was reduced to 50% by 14 days and 0% at 21 days. There were no significant differences in root protein, catalase activity, root death index or total N loss in leachate over 21 days, but by 28 days total N loss in leachate increased to 214% above control levels, with a 433% increase in total oxidized N. This increase in N loss between 21 and 28 days indicated the start of cellular breakdown of the root system, coinciding with the failure of plants to recover.

Key enzyme activities and protein concentrations in nodules decreased rapidly over 10 days' prolonged stress. cDNA–amplified fragment length polymorphism (AFLP) analysis identified contaminating bacterial and fungal genes, along with plant gene sequences with consistent or altered expression profiles. Four plant sequences, glyceraldehyde-3-phosphate dehydrogenase (Tp-gapdh1), nodule senescence reduced (Tp-nsr1), nodule senescence enhanced (Tp-nse1) and a cysteine protease gene (Tp-cp8) were differentially expressed throughout the plant: Tp-nsr1 and Tp-nse1 have potential as molecular markers for nodule senescence.

Root and nodule death in agricultural legumes, such as red clover, are implicated in N release into watercourses and the wider environment. Differences in the ability of these plants to survive prolonged stress lasting 14 days, and the delayed release of root N into leachate until 28 days after the stress, highlight the potential for the development of new red clover varieties with different rates of root system senescence.

When exposed to hot (22–35°C) and dry climatic conditions in the field during the final 4–6 weeks of pod filling, peanuts (Arachis hypogaea L.) can accumulate highly carcinogenic and immuno-suppressing aflatoxins. Forecasting of the risk posed by these conditions can assist in minimizing pre-harvest contamination. A model was therefore developed as part of the Agricultural Production Systems Simulator (APSIM) peanut module, which calculated an aflatoxin risk index (ARI) using four temperature response functions when fractional available soil water was <0·20 and the crop was in the last 0·40 of the pod-filling phase. ARI explained 0·95 (P⩽0·05) of the variation in aflatoxin contamination, which varied from 0 to c. 800 μg/kg in 17 large-scale sowings in tropical and four sowings in sub-tropical environments carried out in Australia between 13 November and 16 December 2007. ARI also explained 0·96 (P⩽0·01) of the variation in the proportion of aflatoxin-contaminated loads (>15 μg/kg) of peanuts in the Kingaroy region of Australia during the period between the 1998/99 and 2007/08 seasons. Simulation of ARI using historical climatic data from 1890 to 2007 indicated a three-fold increase in its value since 1980 compared to the entire previous period. The increase was associated with increases in ambient temperature and decreases in rainfall. To facilitate routine monitoring of aflatoxin risk by growers in near real time, a web interface of the model was also developed. The ARI predicted using this interface for eight growers correlated significantly with the level of contamination in crops (r=0·95, P⩽0·01). These results suggest that ARI simulated by the model is a reliable indicator of aflatoxin contamination that can be used in aflatoxin research as well as a decision-support tool to monitor pre-harvest aflatoxin risk in peanuts.

The present study investigated the effects of maternal plasma iodine concentration on twin- and triplet-born lamb plasma thyroid hormone concentrations, rectal temperature and maximal heat production. On pregnancy day 68 (P68), 16 twin- and 14 triplet-bearing ewes were randomly chosen from ewes that were injected intramuscularly with 1·5 ml of iodized peanut oil and ewes that were not. Selected ewes were grazed on ad libitum pasture from P68 until parturition. After parturition, lamb blood samples were collected within 5 min of birth and at 3, 12 and 24–36 h after birth. Lamb rectal temperatures were measured within 5 min of birth and at 1, 3 and 12 h after birth. Lamb body weight, crown–rump length and thoracic-girth circumference were recorded at 3 h of age, and the capability of the lamb to produce heat at 24–36 h of age was measured using indirect open-circuit calorimetry. Maternal iodine supplementation successfully increased plasma iodine concentrations of twin- and triplet-bearing ewes throughout pregnancy, but had no effect on the rectal temperature, thyroid hormone concentration and maximal heat production of twin- or triplet-born lambs. Compared with twin-born lambs, triplet-born lambs had lower birth weights, rectal temperatures and plasma T4 and T3 concentrations within 5 min of birth. Overall, under the conditions of the present study, maternal iodine supplementation offered no benefit in improving lamb heat production.

The aim of the current study was to compare three methods for determining the influence of different feeding strategies on the gut microbiota of piglets. Forty-eight weanling piglets were fed four different diets enriched with insoluble dietary fibre (wheat bran and pollen from Pinus massoniana). Starting from ileal and colonic samples, the total microbial DNA was isolated and bacterial parameters (lactobacilli, bifidobacteria, Bacteroides vulgatus and total bacterial counts) were quantified using real-time polymerase chain reaction (PCR). The results for lactobacilli, bifidobacteria and total bacterial counts were compared with those obtained by fluorescence in situ hybridization (FISH) and cultivation method. No significant differences could be observed between dietary treatments with real-time PCR and FISH for all investigated parameters. Comparing the applied three methods no consistent results were achieved, whereas FISH usually showed lower values. It was shown that real-time PCR can be regarded as an alternative to conventional techniques and also as a complement to results obtained from conventional culture method.

The effects of deficit irrigation (DI) and partial rootzone drying (PRD) on the growth and mineral nutrition of citrus rootstock seedlings in the glasshouse were determined, as well as the potential of DI and PRD to trigger root-to-shoot signalling of abscisic acid (ABA) to increase the growth per amount of water used (water use efficiency (WUE)). In the DI study, 3-month-old seedlings of the important citrus rootstock Swingle citrumelo with intact roots received three irrigation treatments: control (1·00 evapotranspiration (ET)), 0·75 ET and 0·50 ET. DI clearly decreased growth, the net assimilation of CO2 (ACO2), WUE and the total content of N and K in leaves, even though concentrations of leaf N and K were increased in the drought-stressed smaller plants. Root K was not affected by DI treatments. Leaf ABA concentration increased linearly with DI. For the PRD study, root systems of 6-month-old Swingle citrumelo were split into half and allowed to become established in adjacent pots. There were three irrigation treatments: control (1·00 of the total crop ET, 0·50 in each pot), PRD 50-0 (0·50 ET by weight applied to only one-half of root zone) and DI 25-25 (0·50 ET in total, with 0·25 ET applied to each root half). Although the total root length was decreased by the DI 25-25 treatment, PRD 50-0 did not affect any growth characteristics compared to control plants. The dry root zone of the PRD 50-0 treatment had a higher specific root length, longer roots per dry weight, than the wet root zone. Leaf ACO2 and WUE of the DI 25-25 treatment were significantly lower than control plants after 11 weeks. Although the total contents of N and K in leaves were not affected by either PRD treatment, the concentrations of N and K in leaves were increased by DI 25-25. Root K was decreased by PRD treatments. Leaf ABA concentration was increased by PRD 50-0 but not by DI 25-25. Although all drought stress treatments increased the levels of ABA in leaves, DI and PRD treatments did not affect the whole plant WUE. Compared to well-irrigated control plants, DI reduced growth, whereas PRD 50-0 did not.

The current study investigated the effects of dam weight and nutrition during gestation on the reproductive performance of female primiparous offspring at 2 years of age. Four hundred and fifty heavy (H) (mean±s.e.m.: 60·8 kg±0·18) and 450 light (L) (42·5 kg±0·17) dams were randomly allocated to ad libitum (A) or maintenance (M) nutritional regimens from day 21 until day 140 of pregnancy, under pastoral grazing conditions. One week prior to lambing, all dams and their lambs were provided with ad libitum feeding through to weaning. After weaning, female progeny were managed and fed to requirements as one group. At 2 years of age, the oestrous cycles of the female offspring (n=207) were synchronized and the offspring were naturally mated. Ewes were scanned for pregnancy by ultrasound at day 70 of pregnancy. Within 24 h of birth, lambs were weighed and body dimensions were measured. Lambs were also weighed at day 24 (L24) and weaning. No effects of dam nutrition or dam weight were found (P>0·10) on the reproductive performance of the ewe offspring. Lambs of M-grand-dams were heavier at birth (P=0·024) and weaning (P=0·031) than lambs of A-grand-dams. Twin lambs of H-grand-dams were heavier at birth (P=0·014) than twin lambs of L-grand-dams; however, grand-dam weight had no effect (P>0·10) on lamb weaning weight. In summary, dam weight had no effect on reproductive performance of the female offspring, with only a minor effect on the weight of grand-offspring. Thus, being born to a larger dam has no advantages over being born to a smaller dam, in terms of number of lambs born and weight of lambs at birth and weaning. Grand-dam maintenance nutrition had no effect on reproductive performance although it increased lamb birth and weaning weight and lamb growth rates of the grand-offspring. Therefore, this indicates that ewes born to dams fed at maintenance during pregnancy have an advantage over A-ewes in physiological stressful situations including pregnancy or lactation.

The current work was designed to compare 13 agronomic and four physiological traits in mungbean isogenic lines (ILs) with different leaflet sizes and numbers. The IL population was developed from a cross made in 2006 at Kasetsart University, Thailand, between two pure lines (one with five small and the other with seven large leaflets), followed by continuous selfing of the progenies until F6. The resulting four IL families each exhibited seven leaflet types, viz. three normal-sized leaflets per leaf, seven large leaflets, nine large, five small, seven small, nine small or 11 small. A trial was conducted in 2008 to compare the 28 ILs using a randomized complete block design with three replications. The large multiple-leaflet lines gave higher values for seeds/pod, pod size, seed weight, seed yield, leaf area, leaf area index and proportion of light interception, but fewer clusters of pods, branches and pods per plant than the small multiple-leaflet ILs. Seed yield/plant was positively correlated with pods/plant, seeds/pod, mean seed weight, light interception, leaf area and plant height at maturity. Leaflet size showed association with more traits than did leaflet number.

The aim of the current study was to determine how cooling and supplemental recombinant bovine somatotropin (rbST) affect body function with respect to digestion kinetics, digestibility and other variables relevant to milk production in cross-bred Holstein cattle. Ten primiparous cross-bred dairy cattle (0·875 Holstein Friesian×0·125 Red Shindi) were used and divided into two groups of five animals each that were housed in a normal shaded barn (NS barn; non-cooled cows) and in a shaded barn with mist-fan cooling (MF; cooled cows). The cows in each group were supplemented with rbST in early, mid and late stages of lactation with three consecutive subcutaneous injections of 500 mg rbST every 14 days. All cows were fed the same total mixed ration twice daily at approximately 1·1 of assumed ad libitum intake and water was offered ad libitum. During the experimental periods, values of ambient temperatures and temperature humidity index (THI) in the NS barn were significantly higher than in the MF barn, whereas the relative humidity in the MF barn was significantly higher than in the NS barn (P<0·01). The respiration rate and rectal temperature were significantly higher for non-cooled cows than for cooled cows during the daytime whether there was or was not rbST supplementation. Supplementation of rbST for either cooled or non-cooled cows significantly increased dry matter intake (DMI), the efficiency of feed utilization and milk yields (P<0·05). Digesta kinetics using chromic oxide as an external marker showed a high digesta passage rate constant and low mean retention time of digesta in cows either by cooling or supplementation of rbST, whereas no changes were seen for the digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF). The half-time of Cr2O3 in the whole digestive tract of cooled cows was lower than those of non-cooled cows and significantly decreased (P<0·05) during rbST supplementation in both groups in all stages of lactation. The magnitude of responses for the digesta passage rate and efficiency of feed utilization were larger in animals supplemented with rbST than in animals under MF cooling only.

The main effect of cooling and supplemental rbST was to improve digestion by an increase in the rate of passage of digesta and in turn an increase in feed intake. Digestibility was not influenced by changes in passage rate of digesta either by cooling or rbST supplementation. Milk production in response to rbST supplementation is probably enhanced with cooling. The increased milk production induced by rbST supplementation was mediated by increased efficiency of feed utilization without changes in diet digestibility.

In order to assess the diet of lactating ewes fed at pasture and the relationship of diet quality to pasture characteristics and milk production (MP), 12 lactating ewes were monitored during five lactation cycles (LCs). Individual faecal samples were collected three times per LC and scanned using a Foss NIRSystem 6500 monochromator. The organic matter digestibility (OMD), organic matter intake (OMI), digestible OMI (DOMI) and the chemical composition of the herbage ingested, i.e. the crude protein (CP) content of the herbage ingested (CPi) and the acid detergent fibre and lignin contents of the herbage ingested (ADFi and ADLi), were determined. The MP of the ewes and the pasture characteristics were measured and their relationships with diet quality were analysed.

OMI and DOMI were higher during the first and the fourth LC (P<0·01). OMD and CPi were higher during the second and the fifth LC (P<0·01). MP varied with OMI (P<0·01) and with DOMI (MP=0·1362×DOMI0·53, R2=0·40, P<0·001), whereas it was negatively correlated with OMD (r=−0·46, P<0·001). OMD and CPi were both positively correlated with the CP content of the pasture (r=0·25, P<0·03; r=0·50, P<0·001), whereas the ADFi was negatively correlated with CP content (r=−0·48, P<0·01). The variation in OMI, DOMI and MP, on the one hand, and OMD and CPi, on the other, throughout the different LCs illustrates the importance of measuring several parameters when evaluating diets at pasture.

A possible outcome of policies designed to reduce obesity in the human population and to mitigate greenhouse gas (GHG) emissions may be a decrease in human consumption of livestock products. However, livestock products currently make substantial contributions to intakes of specific micro-nutrients. Therefore, the present review examines the potential for increasing micro-nutrient concentrations of milk, muscle meats and eggs by nutritional and genetic means. Of the trace elements, copper (Cu), iron (Fe) and zinc (Zn) concentrations were largely resistant to manipulation by dietary means, but iodine (I) and selenium (Se) could be readily manipulated. Similarly, while α-tocopherol concentrations were readily manipulated, responses to dietary supplementation with retinol, folate and cobalamin were lower and riboflavin was resistant to dietary manipulation. There were differences between products in the ease with which composition could be manipulated: egg concentrations were most responsive followed by milk and muscle meats. However, livestock products with increased micro-nutrients concentrations can supply a substantial proportion of the daily reference nutrient intake.

The objective of the present study was to estimate genetic parameters for body weights at different ages and litter size (LS) in Moghani sheep. Traits included were birth weight (BW), 3 months weight (3MW), 6 months weight (6MW), 9 months weight (9MW), yearling weight (YW) and LS. The data of 6659 lambing records and pedigree information used in the current programme were collected at the Breeding Station of Moghani sheep (Ardebil province, Iran) during 1987–2005. Different linear and threshold animal models with additive genetic, maternal genetic, maternal permanent environmental and residual effects were implemented by Gibbs sampling (GS) methodology. A single GS chain with 150 000 rounds was generated by the MTGSAM program. The posterior means of genetic parameters were estimated based on the 1300 samples that were left after elimination of 20 000 rounds in the burn-in period and 100 rounds of each thinning interval. Posterior mean estimates of direct heritability were 0·29, 0·13, 0·14, 0·10, 0·31 and 0·10, while those of maternal heritabilities were 0·29, 0·08, 0·11, 0·06, 0·10 and 0·17 for BW, 3MW, 6MW, 9MW, YW and LS, respectively. Genetic correlations among the growth traits and LS were negative for direct genetic and maternal genetic effects. Therefore, selection for increased growth or LS may have a negative genetic effect on the other traits. The medium to high negative estimates of direct–maternal correlations for body weight traits or LS suggest that it would be difficult to improve direct and maternal growth ability jointly for Moghani sheep.