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Exploring the Origin and Genetic Diversity of the Giant Reed, Arundo donax in South Africa

  • Kim Canavan (a1), Iain D. Paterson (a1) and Martin P. Hill (a1)


The giant reed, Arundo donax is one of the worst invasive alien species globally, including South Africa, where it invades riparian areas across the country. Biological control is being considered to address the invasive potential and negative impacts of the weed. This study investigated the phylogeography of A. donax to guide the biological control program. To determine plant haplotype and genetic diversity, three regions of the chloroplast were sequenced and three microsatellite markers were analyzed in 40 samples from across the plant’s distribution in South Africa. It was determined that all populations of A. donax in South Africa were haplotype M1, which is the most widely distributed haplotype worldwide, believed to originate from the Indus Valley, Asia. In addition, no genetic diversity was found, indicating that all the A. donax populations in South Africa are essentially one clone. The results indicate that suitable biological control agents are likely to be found in the ancient native range of haplotype M1. This research has contributed to the global understanding of the phylogeography of A. donax and will guide the biological control program in South Africa.


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Associate Editor: Jacob N. Barney, Virginia Tech.



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Ahmad, R, Liow, P, Spencer, DF, Jasieniuk, M (2008) Molecular evidence for a single genetic clone of invasive Arundo donax in the United States. Aquat Bot 88:113120
Angelini, LG, Ceccarini, L, Bonari, E (2005) Biomass yield and energy balance of giant reed (Arundo donax L.) cropped in central Italy as related to different management practices. Eur J Agron 22:375389
Baker, HHG (1967) Support for Baker’s law-as a rule. Evolution (NY) 21:853856
Barker, NP, Linder, HP, Harley, EH (1995) Polyphyly of Arundinoideae (Poaceae): evidence from rbcL sequence data. Syst Bot 20:423435
Bell, GP (1997) Ecology and management of Arundo donax, and approaches to riparian habitat restoration in southern California. Pages 103113 in Brock J, Wade M, Pyšek P & Green D eds. Plant Invasions: Studies from North America and Europe. Leiden, Netherlands: Backhuys
Bhattarai, GP (2015) Biogeographical approaches for studying species invasion. Ph.D dissertation. Baton Rouge, LA: Louisiana State University
Bonin, A, Bellemain, E, Bronken Eidesen, P, Pompanon, F, Brochmann, C, Taberlet, P (2004) How to track and assess genotyping errors in population genetics studies. Mol Ecol 13:32613273
Burdon, JJ, Marshall, DR (1981) Biological control and the reproductive mode of weeds. J Appl Ecol 18:649658
Calheiros, CSC, Quitério, PVB, Silva, G, Crispim, LFC, Brix, H, Moura, SC, Castro, PML (2012) Use of constructed wetland systems with Arundo and Sarcocornia for polishing high salinity tannery wastewater. J Environ Manage 95:6671
Chapman, HM, Parh, D, Oraguzie, N (2000) Genetic structure and colonizing success of a clonal, weedy species, Pilosella officinarum (Asteraceae). Heredity (Edinb) 84:401409
Chase, MW, Hills, HH (1991) Silica gel: an ideal material for field preservation of leaf samples for DNA studies. Taxon 40:215220
Cortes, E, Goolsby, JA, Moran, PJ, Marcos-García, MA (2011) The effect of the armored scale, Rhizaspidiotus donacis (Hemiptera: Diaspididae), on shoot growth of the invasive plant Arundo donax (Poaceae: Arundinoideae). Biocontrol Sci Technol 21:535545
Cosentino, SL, Copani, V, D’Agosta, GM, Sanzone, E, Mantineo, M (2006) First results on evaluation of Arundo donax L. clones collected in Southern Italy. Ind Crops Prod 23:212222
Cronin, JT, Kiviat, E, Meyerson, LA, Bhattarai, GP, Allen, WJ (2016) Biological control of invasive Phragmites australis will be detrimental to native P. australis . Biol Invasions 18:27492752
Dudley, TL (2000) Arundo donax L. Pages 5358 in Bossard CC, Randall JM & Hoshovsky MC eds. Invasive Plants of : California’s Wildlands. Berkeley: University of California Press
Else, JA (1996) Post-flood establishment of native woody species and an exotic, Arundo donax, in a southern Californian riparian system. M.Sc. dissertation. San Diego, CA: San Diego State University
Ehrlich, PR, Raven, PH (1964) Butterflies and plants: a study in coevolution. Evolution (NY) 18:586608
Fallour, D, Fady, B, Lefevre, F (1997) Study on isozyme variation in Pinus pinea L.: evidence for low polymorphism. Silvae Genet 46:201206
Fish, L, Mashau, A, Moeaha, M, Nembudani, M (2015) Identification Guide to Southern African Grasses. An Identification Manual with Keys, Descriptions and Distributions. Pretoria: South African National Biodiversity Institute
Flores, A, Wood, M (2009) Biocontrol battle begins against giant reed (Arundo). Agric Res 57:1214
Fritz, R, Simms, E (1992) Plant resistance to herbivores and pathogens: ecology, evolution, and genetics. Science 259:16311632
Godt, MJW, Walker, J, Hamrick, JL (1997) Genetic diversity in the endangered lily Harperocallis flava and a close relative Tofieldia racemosa . Conserv Biol 11:361366
Goolsby, JA, De Barro, PJ, Makinson, JR, Pemberton, RW, Hartley, DM, Frohlich, DR (2006) Matching the origin of an invasive weed for selection of a herbivore haplotype for a biological control programme. Mol Ecol 15:287297
Goolsby, JA, Kirk, AA, Moran, PJ, Racelis, AE, Adamczyk, JJ, Cortes, E, Marcos, MA, Jimenez, MM, Summy, KR, Ciomperlik, MA, Sands, DPA (2011) Establishment of the armored scale, Rhizaspidiotus donacis, a biological control agent of Arundo donax . Southwest Entomol 36:373374
Goolsby, JA, Moran, PJ, Adamczyk, JJ, Kirk, AA, Jones, WA, Marcos, MA, Cortés, E (2009) Host range of the European, rhizome-stem feeding scale Rhizaspidiotus donacis (Hemiptera: Diaspididae), a candidate biological control agent for giant reed, Arundo donax (Poales: Poaceae) in North America. Biocontrol Sci Technol 19:899918
Groves, RH, Di Castri, F (1991) Biogeography of Mediterranean Invasions. Cambridge: Cambridge University Press
Guthrie, G (2007) Impacts of the invasive reed Arundo donax on biodiversity at the community-ecosystem level. M.Sc. dissertation. Cape Town, Western Cape: University of the Western Cape
Haddadchi, A, Gross, CL, Fatemi, M (2013) The expansion of sterile Arundo donax (Poaceae) in southeastern Australia is accompanied by genotypic variation. Aquat Bot 104:153161
Hardion, L, Verlaque, R, Baumel, A, Juin, M, Vila, B (2012) Revised systematics of Mediterranean Arundo (Poaceae) based on AFLP fingerprints and morphology. Taxon 61:12171226
Hardion, L, Verlaque, R, Saltonstall, K, Leriche, A, Vila, B (2014) Origin of the invasive Arundo donax (Poaceae): a trans-Asian expedition in herbaria. Ann Bot 114:455462
Henderson, L (2001) Alien Weeds and Invasive Plants. A complete guide to declared weeds and invaders in South Africa. Pretoria: Agricultural Research Council, Plant Protection Research Institute
Holt, RD (2003) On the evolutionary ecology of species’ ranges. Evol Ecol Res 5:159178
Kearse, M, Moir, R, Wilson, A, Stones-Havas, S, Cheung, M, Sturrock, S, Buxton, S, Cooper, A, Markowitz, S, Duran, C, Thierer, T, Ashton, B, Meintjes, P, Drummond, A (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:16471649
Khudamrongsawat, J, Tayyar, R, Holt, JS (2009) Genetic diversity of giant reed (Arundo donax) in the Santa Ana River, California. Weed Sci 52:395405
Kirkpatrick, M, Barton, NH (1997) Evolution of a species’ range. Am Nat 150:123
Kumar, S, Stecher, G, Peterson, D, Tamura, K (2012) MEGA-CC: computing core of molecular evolutionary genetics analysis program for automated and iterative data analysis. Bioinformatics 28:26852686
Lambert, AM, Casagrande, RA (2007) Susceptibility of native and non-native common reed to the non-native mealy plum aphid (Homoptera: Aphididae) in North America. Environ Entomol 36:451457
Lavergne, S, Molofsky, J (2007) Increased genetic variation and evolutionary potential drive the success of an invasive grass. Proc Natl Acad Sci USA 104:38833888
Le Roux, JJ, Wieczorek, AM, Wright, MG, Tran, CT (2007) Super-genotype: global monoclonality defies the odds of nature. PLoS One 2:e590
Lewandowski, I, Scurlock, JMO, Lindvall, E, Christou, M (2003) The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe. Biomass Bioenergy 25:335361
Linder, HP, Verboom, GA, Barker, NP (1997) Phylogeny and evolution in the Crinipes Group of grasses (Arundinoideae: Poaceae). Kew Bull 52:91110
Mabberley, DJ (1997) The Plant-Book. Cambridge: Cambridge University Press
Mariani, C, Cabrini, R, Danin, A, Piffanelli, P, Fricano, A, Gomarasca, S, Dicandilo, M, Grassi, F, Soave, C (2010) Origin, diffusion and reproduction of the giant reed (Arundo donax L.): a promising weedy energy crop. Ann Appl Biol 157:191202
Milton, SJ (2004) Grasses as invasive alien plants in South Africa: working for water. S Afr J Sci 100:6975
Moore, GW, Watts, DA, Goolsby, JA (2010) Ecophysiological responses of giant reed (Arundo donax) to herbivory. Invasive Plant Sci Manag 3:521529
Moran, PJ, Goolsby, JA, Racelis, AE, Cohen, AC, Ciomperlik, MA, Summy, KR, Sands, DP, Kirk, AA (2013) Mass rearing of the stem-galling wasp Tetramesa romana, a biological control agent of the invasive weed Arundo donax . Pages 163201 in Morales-Ramos JA, Shapiro-Ilan D & Rojas G eds. Mass-Rearing of Beneficial Organisms. San Diego, CA: Elsevier
Muller-Scharer, H, Schaffner, U, Steinger, T (2004) Evolution in invasive plants: implications for biological control. Trends Ecol Evol 19:417422
Parker, ED (1979) Ecological implications of clonal diversity in parthenogenetic morphospecies. Integr Comp Biol 19:753762
Peakall, R, Smouse, PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28:25372539
Perdue, RE (1958) Arundo donax—source of musical reeds and industrial cellulose. Econ Bot 12:368404
Pérez de la Vega, M, García, P, Allard, RW (1991) Multilocus genetic structure of ancestral Spanish and colonial Californian populations of Avena barbata . Proc Natl Acad Sci USA 88:12021206
Pyšek, P, Richardson, DM, Rejmánek, M, Webster, GL, Williamson, M, Kirschner, J (2004) Alien plants in checklists and floras: towards better communication between taxonomists and ecologists. Taxon 53:131143
Quinn, LD, Holt, JS (2008) Ecological correlates of invasion by Arundo donax in three southern California riparian habitats. Biol Invasions 10:591601
Roderick, GK (2004) Tracing the origin of pests and natural enemies: genetic and statistical approaches. Pages 97112 in Ehler LE, Sforza R & Mateille T eds. Genetics, Evolution and Biological Control. CAB eBooks
Rouget, M, Richardson, DM, Nel, JL, Le Maitre, DC, Egoh, B, Mgidi, T (2004) Mapping the potential ranges of major plant invaders in South Africa, Lesotho and Swaziland using climatic suitability. Divers Distrib 10:475484
Saltonstall, K (2001) A set of primers for amplification of noncoding regions of chloroplast DNA in the grasses. Mol Ecol Notes 1:7678
Saltonstall, K (2002) Cryptic invasion by a non-native genotype of the common reed, Phragmites australis, into North America. Proc Natl Acad Sci USA 99:24452449
Saltonstall, K, Lambert, A, Meyerson, LA (2010) Genetics and reproduction of common (Phragmites australis) and giant reed (Arundo donax). Invasive Plant Sci Manag 3:495505
Seawright, EK, Rister, ME, Lacewell, RD, McCorkle, DA, Sturdivant, AW, Yang, C, Goolsby, JA (2009) Economic implications for the biological control of Arundo donax: Rio Grande Basin. Southwest Entomol 34:377394
Taberlet, P, Gielly, L, Pautou, G, Bouvet, J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:11051109
Tarin, D, Pepper, AE, Goolsby, JA, Moran, PJ, Arquieta, AC, Kirk, AE, Manhart, JR (2013) Microsatellites uncover multiple introductions of clonal giant reed (Arundo donax). Invasive Plant Sci Manag 6:328338
Thompson, JD, McNeilly, T, Gray, AJ (1991) Population variation in Spartina anglica C. E. Hubbard. II. Reciprocal transplants among three successional populations. New Phytol 117:129139
Tracy, JL, DeLoach, CJ (1998) Suitability of classical biological control for giant reed (Arundo donax) in the United States. Pages 73–109 in Arundo and Saltcedar Management Workshop Proceedings. Holtville: University of California Cooperative Extension
Van der Merwe, CG, Schoonbee, HJ, Pretorius, J (1990) Observations on concentrations of the heavy metals zinc, manganese, nickel and iron in the water, in the sediments and in two aquatic macrophytes, Typha capensis (Rohrb.) N. E. Br. and Arundo donax L., of a stream affected by goldmine and industrial effluent. Water South Africa 16:119124
Van Doninck, K, Schön, I, De Bruyn, L, Martens, K (2002) A general purpose genotype in an ancient asexual. Oecologia 132:205212
van Wilgen, BW, Nel, JL, Rouget, M (2007) Invasive alien plants and South African rivers: a proposed approach to the prioritization of control operations. Freshw Biol 52:711723
Vendramin, GG, Fady, B, González-Martínez, SC, Hu, FS, Scotti, I, Sebastiani, F, Soto, A, Petit, R, Vendramin, GG, Fady, B, et al. (2008) Genetically depauperate but widespread: the case of an emblematic Mediterranean pine. Evolution (NY) 62:680688


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Exploring the Origin and Genetic Diversity of the Giant Reed, Arundo donax in South Africa

  • Kim Canavan (a1), Iain D. Paterson (a1) and Martin P. Hill (a1)


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