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1175 – The Effect Of Mk-801 On The Erk-creb Signal System In Prenatally Stressed Offspring Rats

  • L. Guan (a1), X. Zhao (a1), X. Zhang (a1), H. Sun (a1), D. Wang (a2), Q. Su (a3), G. Tang (a2), L. Yang (a3), Q. Song (a2), N. Jia (a4), H. Li (a5) and Z. Zhu (a1) (a2)...

Abstract

Introduction

Studies revealed that prenatal stress (PS) may increase the vulnerability to depression in their offspring, and ERK-CREB signal system might play a role in its mechanism.

Objectives and aims

The present study investigated the effect of MK-801 on depressive-like behavior and its impacts on ERK2, CREB, Bcl-2 mRNA expression in PS female rat offspring.

Methods

The pregnant rats were randomly divided into three groups, the control group (Con) was left undisturbed, the PS-saline group (PS-saline) and the PS-MK-801 group (PS-MK-801) were subjected to restraint stress on days 14–20 of pregnancy three times daily for 45 min, and received an i.p. administration of saline or MK-801(sigma, 0.2 mg/kg) 30 min before the first stress respectively. Forced swimming test was undertaken to assess depressive-like behavior in one month female offspring. ERK2, CREB, Bcl-2 mRNA in the hippocampus, frontal cortex, and striatum were detected by RT-PCR.

Results

PS-saline spent significantly more immobile time compared to Con and PS-MK-801 (P < 0.05). ERK2 and CREB mRNA expression in hippocampus and frontal cortex was significantly decreased in PS-saline compared to Con and PS-MK-801 (P < 0.05), while in striatum CREB mRNA expression in PS-saline was lower than Con (P < 0.05). Bcl-2 mRNA expression in hippocampus and striatum was significantly decreased in PS-saline (P < 0.05), and in frontal cortex, its expression was significantly lower in PS-saline and PS-MK-801 (P < 0.05).

Conclusions

PS may suppress ERK-CREB signal pathway in female offspring rats, which could be partly prevented by MK- 801. (Supported by National Natural Science Foundation of China, No: 30970952).

Copyright

1175 – The Effect Of Mk-801 On The Erk-creb Signal System In Prenatally Stressed Offspring Rats

  • L. Guan (a1), X. Zhao (a1), X. Zhang (a1), H. Sun (a1), D. Wang (a2), Q. Su (a3), G. Tang (a2), L. Yang (a3), Q. Song (a2), N. Jia (a4), H. Li (a5) and Z. Zhu (a1) (a2)...

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1175 – The Effect Of Mk-801 On The Erk-creb Signal System In Prenatally Stressed Offspring Rats

  • L. Guan (a1), X. Zhao (a1), X. Zhang (a1), H. Sun (a1), D. Wang (a2), Q. Su (a3), G. Tang (a2), L. Yang (a3), Q. Song (a2), N. Jia (a4), H. Li (a5) and Z. Zhu (a1) (a2)...
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