Skip to main content Accessibility help
×
Home

Molecular typing of adenovirus circulating in a Colombian paediatric population with acute respiratory infection

  • L. J. ROJAS (a1), C. A. JARAMILLO (a1), M. F. MOJICA (a1), M. P. ESCALANTE (a2) and P. DELGADO (a1)...

Summary

Human adenoviruses (HAdV) cause a broad spectrum of diseases including acute respiratory infection (ARI), and are responsible for 5% of cases requiring hospitalization in children aged <5 years in Colombia; however, little is known about the circulating types, partly due to the lack of reliable typing tests. In order to evaluate a VA gene PCR-sequencing approach for identification of HAdV circulating types in a Colombian population, 52 nasopharyngeal aspirates/swabs from children with ARI were processed. After a BLAST analysis, matches with species B (41/48, 85·42%), C (6/48, 12·5%), and D (1/48, 2·08%) were found; and at the type level, type 3 (22/48, 45·83%) was the most frequent. This initial effort to expand our knowledge about the molecular epidemiology of HAdV circulating in Bogota, Colombia, showed that HAdV-B was the predominant circulating species in the study period and reports, for the first time in Colombia, the presence of HAdV-D in a respiratory sample.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Molecular typing of adenovirus circulating in a Colombian paediatric population with acute respiratory infection
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Molecular typing of adenovirus circulating in a Colombian paediatric population with acute respiratory infection
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Molecular typing of adenovirus circulating in a Colombian paediatric population with acute respiratory infection
      Available formats
      ×

Copyright

Corresponding author

*Author for correspondence: Dr P. Delgado, Laboratorio de Diagnóstico Molecular y Bioinformática, Universidad de los Andes, Carrera 1 N° 18A 10 Edificio J (J203), Bogotá, Colombia. (Email: mdelgado@uniandes.edu.co)

References

Hide All
1.Benkö, M, et al. Family Adenoviridae. In: Fauquet, C, et al. , eds. VIIIth Report of the International Committee on Taxonomy of Viruses. New York: Elservier, 2005, pp. 213228.
2.Carstens, E. Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009). Archives of Virology 2010; 155: 133146.
3.Madisch, I, et al. Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy. Journal of Virology 2005; 79: 1526515276.
4.Echavarria, M. Adenovirus. In: Zuckerman, A, Banatvala, J, Griffiths, P, eds. Principles and Practice of Clinical Virology. Chichester: Wiley, 2009, pp. 463488.
5.World, W, Horwitz, M. Adenoviruses. In: Knipe, D, Howley, PM, eds. Fields Virology, 5th edn. Philadelphia: Lippincott Williams & Wilkins, 2007, pp. 23952436.
6.Jones, MS, et al. New adenovirus species found in a patient presenting with gastroenteritis. Journal of Virology 2007; 81: 59785984.
7.WHO. Family and Community Health Cluster (FCH): Acute respiratory infections in children (http://www.who.int/fch/depts/cah/resp_infections/en/). Accessed 6 March 2011.
8.Kim, Y, et al. Genome type analysis of Adenovirus types 3 and 7 isolated during successive outbreaks of lower respiratory tract infections in children. Journal of Clinical Microbiology 2003; 41: 45944599.
9.Herrera, D, De la Hoz, F, Velandia, M. Severe respiratory disease and its relationship with respiratory viruses in Colombia. International Journal of Infectious Diseases 2008; 12: 139142.
10.Sarantis, H, et al. Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing. Journal of Clinical Microbiology 2004; 42: 39633969.
11.Kidd, A, et al. Rapid subgenus identification of human adenovirus isolates by a general PCR. Journal of Clinical Microbiology 1996; 34: 622627.
12.Lu, X, Erman, D. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Archives of Virology 2006; 151: 15871602.
13.Coventry, V, Conn, G. Analysis of adenovirus VA RNAI structure and stability using compensatory base pair modification. Nucleic Acids Research 2008; 36: 16451653.
14.Hall, T. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series 1999; 41: 9598.
15.Altschul, S, et al. Basic local alignment search tool. Journal of Molecular Biology 1990; 215: 403410.
16.Kusznierz, G, et al. Adenovirus acute respiratory infection in hospitalized patients in Santa Fe [in Spanish]. Archivos Argentinos de Pediatría 2007; 105: 216220.
17.Pumariega, T, et al. Isolation and identification of Adenovirus in hospitalized children, under five years, with acute respiratory disease, in Havana, Cuba. Memórias do Instituto Oswaldo Cruz 2000; 95: 859861.
18.Moura, F, et al. Antigenic and genomic characterization of adenovirus associated with respiratory infections in children living in Northeast Brazil. Memórias do Instituto Oswaldo Cruz 2007; 102: 937941.
19.Saiki, RK, et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985; 230: 13501354.
20.Piñón, A. Diagnostic general techniques: immunofluorescence. In: Savón, C, et al. Guide for Diagnostic Laboratory Procedures of Acute Respiratory Infections of Viral Etiology. Washington: Pan American Health Organization (PAHO), 2003, pp. 3744.
21.Ma, Y, Mathews, M. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. Journal of Virology 1996; 70: 50835099.
22.Vabret, A, et al. Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus®) for the detection and the species identification of adenoviruses in respiratory specimens. Journal of Clinical Virology 2004; 31: 116122.
23.Kajon, A, et al. Molecular epidemiology of adenovirus acute lower respiratory infections of children in the South Cone of South America (1991–1994). Journal of Medical Virology 1996; 48: 151156.
24.Xu, W, Erdman, D. Type-specific identification of human adenovirus 3, 7, and 21 by a multiplex PCR assay. Journal of Medical Virology 2001; 64: 537542.
25.García, J, et al. Molecular characterization of adenovirus circulating in Central and South America during the 2006–2008 period. Influenza and Other Respiratory Viruses 2009; 3: 327330.
26.Rosete, DP, Manjarrez, ME, Barrón, BL. Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection. Memórias do Instituto Oswaldo Cruz 2008; 103: 195200.
27.Herrera-Rodríguez, D, et al. Adenovirus in children under five years. Circulation patterns, clinical and epidemiological characteristics in Colombia, 1997–2003 [in Spanish]. Revista de Salud Pública 2007; 9: 420429.

Keywords

Molecular typing of adenovirus circulating in a Colombian paediatric population with acute respiratory infection

  • L. J. ROJAS (a1), C. A. JARAMILLO (a1), M. F. MOJICA (a1), M. P. ESCALANTE (a2) and P. DELGADO (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed